Stimulation of pneumovirus-specific CD8(+) T-cells using a non-toxic recombinant ricin delivery system

E. Grimaldi, E.A.W. Claassen

    Research output: Contribution to journalArticleAcademicpeer-review

    2 Citations (Scopus)

    Abstract

    Internalisation of the plant toxin ricin occurs by retrograde transport which delivers the toxin to the ER where it intersects with the MHC class I system for peptide antigen display. Here, we describe the generation of an inactivated, non-toxic, ricin molecule fused to a peptide which elicits a CD8+ T-cell response in mice directed against pneumonia virus of mice, a pneumovirus related to human respiratory syncytial virus. The ricin fusion elicited a significant T-cell response when delivered by intraperitoneal inoculation in the absence of adjuvent. Challenge experiments showed that the T-cell response resulting from inoculation with the ricin-peptide fusion molecule delayed the onset of virus-induced disease.
    Original languageEnglish
    Pages (from-to)993-998
    Number of pages6
    JournalMolecular Immunology
    Volume44
    Issue number5
    DOIs
    Publication statusPublished - 2007

    Keywords

    • respiratory syncytial virus
    • pneumonia virus
    • class-i
    • mice
    • infection
    • pathogenesis
    • vaccines
    • peptide

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