Abstract
Internalisation of the plant toxin ricin occurs by retrograde transport which delivers the toxin to the ER where it intersects with the MHC class I system for peptide antigen display. Here, we describe the generation of an inactivated, non-toxic, ricin molecule fused to a peptide which elicits a CD8+ T-cell response in mice directed against pneumonia virus of mice, a pneumovirus related to human respiratory syncytial virus. The ricin fusion elicited a significant T-cell response when delivered by intraperitoneal inoculation in the absence of adjuvent. Challenge experiments showed that the T-cell response resulting from inoculation with the ricin-peptide fusion molecule delayed the onset of virus-induced disease.
Original language | English |
---|---|
Pages (from-to) | 993-998 |
Number of pages | 6 |
Journal | Molecular Immunology |
Volume | 44 |
Issue number | 5 |
DOIs | |
Publication status | Published - 2007 |
Keywords
- respiratory syncytial virus
- pneumonia virus
- class-i
- mice
- infection
- pathogenesis
- vaccines
- peptide