Steviol glycoside rebaudioside A induces glucagon-like peptide-1 and peptide YY release in a porcine ex vivo intestinal model

D. Ripken, N. van der Wielen, H.M. Wortelboer, J. Meijerink, R.F. Witkamp, H.F. Hendriks

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)

Abstract

Glucagon-like peptide-1 (GLP-1) and peptide YY (PYY) are hormones important for satiation and are involved in the process called "ileal brake". The aim of this study was to investigate the GLP-1- and PYY-stimulating efficacy of rebaudioside A, casein, and sucrose. This was studied using tissue segments collected from various regions of the pig small intestine. GLP-1 release was strongest from the distal ileum. There, control release was 0.06 ± 0.01 (GLP-1) and 0.07 ± 0.01 (PYY) pmol/cm(2) of tissue. Rebaudioside A (2.5, 12.5, and 25 mM) stimulated GLP-1 release (0.14 ± 0.02, 0.16 ± 0.02, and 0.13 ± 0.02 pmol/cm(2) of tissue, p <0.001) and PYY release (0.19 ± 0.02, 0.42 ± 0.06, and 0.27 ± 0.03 pmol/cm(2) of tissue, p <0.001). Sucrose stimulated GLP-1 release (0.08 ± 0.01 pmol/cm(2) of tissue, p <0.05) only at 10 mM. Casein (0.5%, 1%, and 2.5%, w/v) stimulated GLP-1 release (0.15 ± 0.03, 0.13 ± 0.02, and 0.14 ± 0.01 pmol/cm(2) of tissue, p <0.001) and PYY release (0.13 ± 0.02, 0.20 ± 0.03, and 0.27 ± 0.03 pmol/cm(2) of tissue, p <0.01). These findings may help in developing dietary approaches for weight management.
Original languageEnglish
Pages (from-to)8365-8370
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume62
Issue number33
DOIs
Publication statusPublished - 2014

Keywords

  • enteroendocrine cell-line
  • nutrient-driven satiety
  • hormone-release
  • taste receptor
  • glp-1 release
  • fatty-acids
  • food-intake
  • secretion
  • gut
  • sweeteners

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