Steviol glycoside rebaudioside A induces glucagon-like peptide-1 and peptide YY release in a porcine ex vivo intestinal model

D. Ripken, N. van der Wielen, H.M. Wortelboer, J. Meijerink, R.F. Witkamp, H.F. Hendriks

Research output: Contribution to journalArticleAcademicpeer-review

11 Citations (Scopus)

Abstract

Glucagon-like peptide-1 (GLP-1) and peptide YY (PYY) are hormones important for satiation and are involved in the process called "ileal brake". The aim of this study was to investigate the GLP-1- and PYY-stimulating efficacy of rebaudioside A, casein, and sucrose. This was studied using tissue segments collected from various regions of the pig small intestine. GLP-1 release was strongest from the distal ileum. There, control release was 0.06 ± 0.01 (GLP-1) and 0.07 ± 0.01 (PYY) pmol/cm(2) of tissue. Rebaudioside A (2.5, 12.5, and 25 mM) stimulated GLP-1 release (0.14 ± 0.02, 0.16 ± 0.02, and 0.13 ± 0.02 pmol/cm(2) of tissue, p <0.001) and PYY release (0.19 ± 0.02, 0.42 ± 0.06, and 0.27 ± 0.03 pmol/cm(2) of tissue, p <0.001). Sucrose stimulated GLP-1 release (0.08 ± 0.01 pmol/cm(2) of tissue, p <0.05) only at 10 mM. Casein (0.5%, 1%, and 2.5%, w/v) stimulated GLP-1 release (0.15 ± 0.03, 0.13 ± 0.02, and 0.14 ± 0.01 pmol/cm(2) of tissue, p <0.001) and PYY release (0.13 ± 0.02, 0.20 ± 0.03, and 0.27 ± 0.03 pmol/cm(2) of tissue, p <0.01). These findings may help in developing dietary approaches for weight management.
Original languageEnglish
Pages (from-to)8365-8370
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume62
Issue number33
DOIs
Publication statusPublished - 2014

Fingerprint

peptide YY
steviol
Peptide YY
glucagon-like peptide 1
Glucagon-Like Peptide 1
Glycosides
glycosides
Swine
Tissue
swine
Caseins
Sucrose
casein
sucrose
Satiation
Peptide Hormones
weight control
satiety
tissues
rebaudioside A

Keywords

  • enteroendocrine cell-line
  • nutrient-driven satiety
  • hormone-release
  • taste receptor
  • glp-1 release
  • fatty-acids
  • food-intake
  • secretion
  • gut
  • sweeteners

Cite this

@article{b65d1a2d80f741cea4d27d08360142a2,
title = "Steviol glycoside rebaudioside A induces glucagon-like peptide-1 and peptide YY release in a porcine ex vivo intestinal model",
abstract = "Glucagon-like peptide-1 (GLP-1) and peptide YY (PYY) are hormones important for satiation and are involved in the process called {"}ileal brake{"}. The aim of this study was to investigate the GLP-1- and PYY-stimulating efficacy of rebaudioside A, casein, and sucrose. This was studied using tissue segments collected from various regions of the pig small intestine. GLP-1 release was strongest from the distal ileum. There, control release was 0.06 ± 0.01 (GLP-1) and 0.07 ± 0.01 (PYY) pmol/cm(2) of tissue. Rebaudioside A (2.5, 12.5, and 25 mM) stimulated GLP-1 release (0.14 ± 0.02, 0.16 ± 0.02, and 0.13 ± 0.02 pmol/cm(2) of tissue, p <0.001) and PYY release (0.19 ± 0.02, 0.42 ± 0.06, and 0.27 ± 0.03 pmol/cm(2) of tissue, p <0.001). Sucrose stimulated GLP-1 release (0.08 ± 0.01 pmol/cm(2) of tissue, p <0.05) only at 10 mM. Casein (0.5{\%}, 1{\%}, and 2.5{\%}, w/v) stimulated GLP-1 release (0.15 ± 0.03, 0.13 ± 0.02, and 0.14 ± 0.01 pmol/cm(2) of tissue, p <0.001) and PYY release (0.13 ± 0.02, 0.20 ± 0.03, and 0.27 ± 0.03 pmol/cm(2) of tissue, p <0.01). These findings may help in developing dietary approaches for weight management.",
keywords = "enteroendocrine cell-line, nutrient-driven satiety, hormone-release, taste receptor, glp-1 release, fatty-acids, food-intake, secretion, gut, sweeteners",
author = "D. Ripken and {van der Wielen}, N. and H.M. Wortelboer and J. Meijerink and R.F. Witkamp and H.F. Hendriks",
year = "2014",
doi = "10.1021/jf501105w",
language = "English",
volume = "62",
pages = "8365--8370",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "33",

}

Steviol glycoside rebaudioside A induces glucagon-like peptide-1 and peptide YY release in a porcine ex vivo intestinal model. / Ripken, D.; van der Wielen, N.; Wortelboer, H.M.; Meijerink, J.; Witkamp, R.F.; Hendriks, H.F.

In: Journal of Agricultural and Food Chemistry, Vol. 62, No. 33, 2014, p. 8365-8370.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Steviol glycoside rebaudioside A induces glucagon-like peptide-1 and peptide YY release in a porcine ex vivo intestinal model

AU - Ripken, D.

AU - van der Wielen, N.

AU - Wortelboer, H.M.

AU - Meijerink, J.

AU - Witkamp, R.F.

AU - Hendriks, H.F.

PY - 2014

Y1 - 2014

N2 - Glucagon-like peptide-1 (GLP-1) and peptide YY (PYY) are hormones important for satiation and are involved in the process called "ileal brake". The aim of this study was to investigate the GLP-1- and PYY-stimulating efficacy of rebaudioside A, casein, and sucrose. This was studied using tissue segments collected from various regions of the pig small intestine. GLP-1 release was strongest from the distal ileum. There, control release was 0.06 ± 0.01 (GLP-1) and 0.07 ± 0.01 (PYY) pmol/cm(2) of tissue. Rebaudioside A (2.5, 12.5, and 25 mM) stimulated GLP-1 release (0.14 ± 0.02, 0.16 ± 0.02, and 0.13 ± 0.02 pmol/cm(2) of tissue, p <0.001) and PYY release (0.19 ± 0.02, 0.42 ± 0.06, and 0.27 ± 0.03 pmol/cm(2) of tissue, p <0.001). Sucrose stimulated GLP-1 release (0.08 ± 0.01 pmol/cm(2) of tissue, p <0.05) only at 10 mM. Casein (0.5%, 1%, and 2.5%, w/v) stimulated GLP-1 release (0.15 ± 0.03, 0.13 ± 0.02, and 0.14 ± 0.01 pmol/cm(2) of tissue, p <0.001) and PYY release (0.13 ± 0.02, 0.20 ± 0.03, and 0.27 ± 0.03 pmol/cm(2) of tissue, p <0.01). These findings may help in developing dietary approaches for weight management.

AB - Glucagon-like peptide-1 (GLP-1) and peptide YY (PYY) are hormones important for satiation and are involved in the process called "ileal brake". The aim of this study was to investigate the GLP-1- and PYY-stimulating efficacy of rebaudioside A, casein, and sucrose. This was studied using tissue segments collected from various regions of the pig small intestine. GLP-1 release was strongest from the distal ileum. There, control release was 0.06 ± 0.01 (GLP-1) and 0.07 ± 0.01 (PYY) pmol/cm(2) of tissue. Rebaudioside A (2.5, 12.5, and 25 mM) stimulated GLP-1 release (0.14 ± 0.02, 0.16 ± 0.02, and 0.13 ± 0.02 pmol/cm(2) of tissue, p <0.001) and PYY release (0.19 ± 0.02, 0.42 ± 0.06, and 0.27 ± 0.03 pmol/cm(2) of tissue, p <0.001). Sucrose stimulated GLP-1 release (0.08 ± 0.01 pmol/cm(2) of tissue, p <0.05) only at 10 mM. Casein (0.5%, 1%, and 2.5%, w/v) stimulated GLP-1 release (0.15 ± 0.03, 0.13 ± 0.02, and 0.14 ± 0.01 pmol/cm(2) of tissue, p <0.001) and PYY release (0.13 ± 0.02, 0.20 ± 0.03, and 0.27 ± 0.03 pmol/cm(2) of tissue, p <0.01). These findings may help in developing dietary approaches for weight management.

KW - enteroendocrine cell-line

KW - nutrient-driven satiety

KW - hormone-release

KW - taste receptor

KW - glp-1 release

KW - fatty-acids

KW - food-intake

KW - secretion

KW - gut

KW - sweeteners

U2 - 10.1021/jf501105w

DO - 10.1021/jf501105w

M3 - Article

VL - 62

SP - 8365

EP - 8370

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 33

ER -