Starch phosphorylation plays an important role in starch biosynthesis

Xuan Xu, Dianka Dees, Annemarie Dechesne, Xing Feng Huang, Richard G.F. Visser, Luisa M. Trindade*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

13 Citations (Scopus)

Abstract

Starch phosphate esters are crucial in starch metabolism and render valuable functionality to starches for various industrial applications. A potato glucan, water dikinase (GWD1) was introduced in tubers of two different potato genetic backgrounds: an amylose-containing line Kardal and the amylose-free mutant amf. In both backgrounds, this resulted in two contrasting effects, a number of plants showed higher phosphate content compared to the respective control, while others lines exhibited lower phosphate content, thereby generating two series of starches with broad-scale variation in phosphate content. The results of systematic analyses on these two series of starches revealed that starch phosphate content strongly influenced starch granule morphology, amylose content, starch fine structure, gelatinization characteristics and freeze-thaw stability of starch gels. Further analyses on the expression level of genes involved in starch metabolism suggested that starch phosphorylation regulates starch synthesis by controlling the carbon flux into starch while simultaneously modulating starch-synthesizing genes.
Original languageEnglish
Pages (from-to)1628-1637
JournalCarbohydrate Polymers
Volume157
DOIs
Publication statusPublished - 2017

Fingerprint

Phosphorylation
Biosynthesis
Starch
Phosphates
Amylose
Metabolism
Genes
Glucans
Industrial applications

Keywords

  • Freeze-thaw stability
  • Glucan
  • Starch metabolism
  • Starch phosphate content
  • Storage starch
  • Water dikinase

Cite this

@article{2421390edbea4b6ea9b8ea6eedd4baf7,
title = "Starch phosphorylation plays an important role in starch biosynthesis",
abstract = "Starch phosphate esters are crucial in starch metabolism and render valuable functionality to starches for various industrial applications. A potato glucan, water dikinase (GWD1) was introduced in tubers of two different potato genetic backgrounds: an amylose-containing line Kardal and the amylose-free mutant amf. In both backgrounds, this resulted in two contrasting effects, a number of plants showed higher phosphate content compared to the respective control, while others lines exhibited lower phosphate content, thereby generating two series of starches with broad-scale variation in phosphate content. The results of systematic analyses on these two series of starches revealed that starch phosphate content strongly influenced starch granule morphology, amylose content, starch fine structure, gelatinization characteristics and freeze-thaw stability of starch gels. Further analyses on the expression level of genes involved in starch metabolism suggested that starch phosphorylation regulates starch synthesis by controlling the carbon flux into starch while simultaneously modulating starch-synthesizing genes.",
keywords = "Freeze-thaw stability, Glucan, Starch metabolism, Starch phosphate content, Storage starch, Water dikinase",
author = "Xuan Xu and Dianka Dees and Annemarie Dechesne and Huang, {Xing Feng} and Visser, {Richard G.F.} and Trindade, {Luisa M.}",
year = "2017",
doi = "10.1016/j.carbpol.2016.11.043",
language = "English",
volume = "157",
pages = "1628--1637",
journal = "Carbohydrate Polymers",
issn = "0144-8617",
publisher = "Elsevier",

}

Starch phosphorylation plays an important role in starch biosynthesis. / Xu, Xuan; Dees, Dianka; Dechesne, Annemarie; Huang, Xing Feng; Visser, Richard G.F.; Trindade, Luisa M.

In: Carbohydrate Polymers, Vol. 157, 2017, p. 1628-1637.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Starch phosphorylation plays an important role in starch biosynthesis

AU - Xu, Xuan

AU - Dees, Dianka

AU - Dechesne, Annemarie

AU - Huang, Xing Feng

AU - Visser, Richard G.F.

AU - Trindade, Luisa M.

PY - 2017

Y1 - 2017

N2 - Starch phosphate esters are crucial in starch metabolism and render valuable functionality to starches for various industrial applications. A potato glucan, water dikinase (GWD1) was introduced in tubers of two different potato genetic backgrounds: an amylose-containing line Kardal and the amylose-free mutant amf. In both backgrounds, this resulted in two contrasting effects, a number of plants showed higher phosphate content compared to the respective control, while others lines exhibited lower phosphate content, thereby generating two series of starches with broad-scale variation in phosphate content. The results of systematic analyses on these two series of starches revealed that starch phosphate content strongly influenced starch granule morphology, amylose content, starch fine structure, gelatinization characteristics and freeze-thaw stability of starch gels. Further analyses on the expression level of genes involved in starch metabolism suggested that starch phosphorylation regulates starch synthesis by controlling the carbon flux into starch while simultaneously modulating starch-synthesizing genes.

AB - Starch phosphate esters are crucial in starch metabolism and render valuable functionality to starches for various industrial applications. A potato glucan, water dikinase (GWD1) was introduced in tubers of two different potato genetic backgrounds: an amylose-containing line Kardal and the amylose-free mutant amf. In both backgrounds, this resulted in two contrasting effects, a number of plants showed higher phosphate content compared to the respective control, while others lines exhibited lower phosphate content, thereby generating two series of starches with broad-scale variation in phosphate content. The results of systematic analyses on these two series of starches revealed that starch phosphate content strongly influenced starch granule morphology, amylose content, starch fine structure, gelatinization characteristics and freeze-thaw stability of starch gels. Further analyses on the expression level of genes involved in starch metabolism suggested that starch phosphorylation regulates starch synthesis by controlling the carbon flux into starch while simultaneously modulating starch-synthesizing genes.

KW - Freeze-thaw stability

KW - Glucan

KW - Starch metabolism

KW - Starch phosphate content

KW - Storage starch

KW - Water dikinase

U2 - 10.1016/j.carbpol.2016.11.043

DO - 10.1016/j.carbpol.2016.11.043

M3 - Article

VL - 157

SP - 1628

EP - 1637

JO - Carbohydrate Polymers

JF - Carbohydrate Polymers

SN - 0144-8617

ER -