TY - JOUR
T1 - Starch phosphorylation plays an important role in starch biosynthesis
AU - Xu, Xuan
AU - Dees, Dianka
AU - Dechesne, Annemarie
AU - Huang, Xing Feng
AU - Visser, Richard G.F.
AU - Trindade, Luisa M.
PY - 2017
Y1 - 2017
N2 - Starch phosphate esters are crucial in starch metabolism and render valuable functionality to starches for various industrial applications. A potato glucan, water dikinase (GWD1) was introduced in tubers of two different potato genetic backgrounds: an amylose-containing line Kardal and the amylose-free mutant amf. In both backgrounds, this resulted in two contrasting effects, a number of plants showed higher phosphate content compared to the respective control, while others lines exhibited lower phosphate content, thereby generating two series of starches with broad-scale variation in phosphate content. The results of systematic analyses on these two series of starches revealed that starch phosphate content strongly influenced starch granule morphology, amylose content, starch fine structure, gelatinization characteristics and freeze-thaw stability of starch gels. Further analyses on the expression level of genes involved in starch metabolism suggested that starch phosphorylation regulates starch synthesis by controlling the carbon flux into starch while simultaneously modulating starch-synthesizing genes.
AB - Starch phosphate esters are crucial in starch metabolism and render valuable functionality to starches for various industrial applications. A potato glucan, water dikinase (GWD1) was introduced in tubers of two different potato genetic backgrounds: an amylose-containing line Kardal and the amylose-free mutant amf. In both backgrounds, this resulted in two contrasting effects, a number of plants showed higher phosphate content compared to the respective control, while others lines exhibited lower phosphate content, thereby generating two series of starches with broad-scale variation in phosphate content. The results of systematic analyses on these two series of starches revealed that starch phosphate content strongly influenced starch granule morphology, amylose content, starch fine structure, gelatinization characteristics and freeze-thaw stability of starch gels. Further analyses on the expression level of genes involved in starch metabolism suggested that starch phosphorylation regulates starch synthesis by controlling the carbon flux into starch while simultaneously modulating starch-synthesizing genes.
KW - Freeze-thaw stability
KW - Glucan
KW - Starch metabolism
KW - Starch phosphate content
KW - Storage starch
KW - Water dikinase
U2 - 10.1016/j.carbpol.2016.11.043
DO - 10.1016/j.carbpol.2016.11.043
M3 - Article
AN - SCOPUS:85005980977
SN - 0144-8617
VL - 157
SP - 1628
EP - 1637
JO - Carbohydrate Polymers
JF - Carbohydrate Polymers
ER -