Starch hydrolysis under low water conditions: a conceptual process design

M.E. van der Veen, S. Veelaert, A.J. van der Goot, R.M. Boom

Research output: Contribution to journalArticleAcademicpeer-review

36 Citations (Scopus)


A process concept is presented for the hydrolysis of starch to glucose in highly concentrated systems. Depending on the moisture content, the process consists of two or three stages. The two-stage process comprises combined thermal and enzymatic liquefaction, followed by enzymatic saccharification. The three-stage process starts with shear induced melting of starch, followed by enzymatic liquefaction and saccharification. At a low moisture content, the shear stress needed to completely melt corn starch is so high that significant enzyme inactivation cannot be avoided, which leads to a need for separating starch melting and liquefaction in two separate processing steps. Assuming the use of currently available enzymes, the final product composition was estimated to contain 69-93% glucose, starting with respectively 65% and 35% dry starch. These results showed that the formation of side-products, mainly isomaltose and isomaltotriose, increased with increasing dry matter content. Increasing the dry matter content from 35% to 65% resulted in increasing reactor productivity of 17%, while the amount of water that should be removed from the system was reduced by 87%. (c) 2005 Elsevier Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)178-186
JournalJournal of Food Engineering
Issue number2
Publication statusPublished - 2006


  • thermostable alpha-amylase
  • niger glucoamylase-i
  • twin-screw extruder
  • enzymatic-hydrolysis
  • extrusion-cooking
  • corn starch
  • reactor
  • kinetics
  • glucose
  • liquefaction

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