TY - JOUR
T1 - Staphylococcal protein a is a key factor in neutrophil extracellular traps formation
AU - Hoppenbrouwers, Tamara
AU - Sultan, Andi R.
AU - Abraham, Tsion E.
AU - Lemmens-den Toom, Nicole A.
AU - Manásková, Silvie Hansenová
AU - van Cappellen, Wiggert A.
AU - Houtsmuller, Adriaan B.
AU - van Wamel, Willem J.B.
AU - de Maat, Moniek P.M.
AU - van Neck, Johan W.
PY - 2018/2/5
Y1 - 2018/2/5
N2 - Staphylococcus aureus are strong inducers of neutrophil extracellular traps (NETs), a defense mechanism of neutrophils against pathogens. Our aim was to explore the role of Protein A in S. aureus-induced NETosis. We determined the Protein A production of four different S. aureus strains and found a direct relationship between the degree of NETosis induction and Protein A production: strains producing higher concentrations of Protein A evoke significantly more NETs. A S. aureus strain in which Protein A as well as a second binding protein for immunoglobulins (Sbi) have been knocked-out (ΔSpA ΔSbi) induced significantly less NETosis than the wild-type strain. NETosis induction by this knockout strain can be rescued by the addition of purified Protein A. Dead S. aureus did not induce NETosis. In conclusion, Protein A is a determinant for NETosis induction by S. aureus.
AB - Staphylococcus aureus are strong inducers of neutrophil extracellular traps (NETs), a defense mechanism of neutrophils against pathogens. Our aim was to explore the role of Protein A in S. aureus-induced NETosis. We determined the Protein A production of four different S. aureus strains and found a direct relationship between the degree of NETosis induction and Protein A production: strains producing higher concentrations of Protein A evoke significantly more NETs. A S. aureus strain in which Protein A as well as a second binding protein for immunoglobulins (Sbi) have been knocked-out (ΔSpA ΔSbi) induced significantly less NETosis than the wild-type strain. NETosis induction by this knockout strain can be rescued by the addition of purified Protein A. Dead S. aureus did not induce NETosis. In conclusion, Protein A is a determinant for NETosis induction by S. aureus.
KW - NETs
KW - Neutrophil extracellular traps
KW - Protein A
KW - S. aureus
KW - SpA
KW - Staphylococcus aureus
U2 - 10.3389/fimmu.2018.00165
DO - 10.3389/fimmu.2018.00165
M3 - Article
AN - SCOPUS:85041809278
SN - 1664-3224
VL - 9
JO - Frontiers in Immunology
JF - Frontiers in Immunology
M1 - 165
ER -