Standardization and application of microsatellite markers for variety identification in tomato and wheat

B. Vosman; R. Cooke; M. Ganal; R. Peeters; P. Isaac; G. Bredemeijer

doi:10.17660/ActaHortic.2001.546.38

Standardization and application of microsatellite markers for variety identification in tomato and wheat

B. Vosman, R. Cooke, M. Ganal, R. Peeters, P. Isaac, G. Bredemeijer

Research output: Contribution to journal › Article › Academic › peer-review

15 Citations (Scopus)

Abstract

The present study is part of a EU project that aims to demonstrate the technical viability of STMS markers for variety identification. As examples two important European crop species, tomato and wheat were chosen. Initially, about 30-40 STMS markers were used to identify a set of 20 good markers per crop and to standardise the methodology and the interpretation of the results in different laboratories. Several systems were used for the detection of STMS polymorphisms. The selected STMS markers are being tested on 500 varieties of each species and databases are being constructed. The first comparisons of data generated by the different laboratories revealed a high degree of agreement. The causes of discrepancies between duplicate samples analysed in different laboratories and precautions to prevent them, are discussed.

Original language	English
Pages (from-to)	307-316
Journal	Acta Horticulturae
Volume	546
DOIs	https://doi.org/10.17660/ActaHortic.2001.546.38
Publication status	Published - 2001

Keywords

Database
Microsatellite
Network
Standardization
STMS markers
Tomato
Wheat

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10.17660/ActaHortic.2001.546.38

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abstract = "The present study is part of a EU project that aims to demonstrate the technical viability of STMS markers for variety identification. As examples two important European crop species, tomato and wheat were chosen. Initially, about 30-40 STMS markers were used to identify a set of 20 good markers per crop and to standardise the methodology and the interpretation of the results in different laboratories. Several systems were used for the detection of STMS polymorphisms. The selected STMS markers are being tested on 500 varieties of each species and databases are being constructed. The first comparisons of data generated by the different laboratories revealed a high degree of agreement. The causes of discrepancies between duplicate samples analysed in different laboratories and precautions to prevent them, are discussed.",

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AU - Vosman, B.

AU - Cooke, R.

AU - Ganal, M.

AU - Peeters, R.

AU - Isaac, P.

AU - Bredemeijer, G.

PY - 2001

Y1 - 2001

N2 - The present study is part of a EU project that aims to demonstrate the technical viability of STMS markers for variety identification. As examples two important European crop species, tomato and wheat were chosen. Initially, about 30-40 STMS markers were used to identify a set of 20 good markers per crop and to standardise the methodology and the interpretation of the results in different laboratories. Several systems were used for the detection of STMS polymorphisms. The selected STMS markers are being tested on 500 varieties of each species and databases are being constructed. The first comparisons of data generated by the different laboratories revealed a high degree of agreement. The causes of discrepancies between duplicate samples analysed in different laboratories and precautions to prevent them, are discussed.

AB - The present study is part of a EU project that aims to demonstrate the technical viability of STMS markers for variety identification. As examples two important European crop species, tomato and wheat were chosen. Initially, about 30-40 STMS markers were used to identify a set of 20 good markers per crop and to standardise the methodology and the interpretation of the results in different laboratories. Several systems were used for the detection of STMS polymorphisms. The selected STMS markers are being tested on 500 varieties of each species and databases are being constructed. The first comparisons of data generated by the different laboratories revealed a high degree of agreement. The causes of discrepancies between duplicate samples analysed in different laboratories and precautions to prevent them, are discussed.

KW - Database

KW - Microsatellite

KW - Network

KW - Standardization

KW - STMS markers

KW - Tomato

KW - Wheat

U2 - 10.17660/ActaHortic.2001.546.38

DO - 10.17660/ActaHortic.2001.546.38

M3 - Article

VL - 546

SP - 307

EP - 316

JO - Acta Horticulturae

JF - Acta Horticulturae

SN - 0567-7572

ER -

Standardization and application of microsatellite markers for variety identification in tomato and wheat

Abstract

Keywords

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