Stable Isotope Probing-RNA Strategy to Study Plant/Fungus Interactions

Amandine Lê Van, Marie Duhamel, Achim Quaiser, Philippe Vandenkoornhuyse*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review


The use of stable-isotope probing (SIP) allows tracing specific labeled substrates into fungi leading to a better understanding of their role in biogeochemical cycles and their relationship with their environment. Stable isotope probing combined with ribosomal RNA molecule, conserved in the three kingdoms of life, and messenger RNA analysis permits the linkage of diversity and function. Here, we describe two methods designed to investigate the interactions between plants and their associated mycorrhizal compartment by tracing carbon flux from the host plant to its symbionts.

Original languageEnglish
Title of host publicationMicrobial Environmental Genomics (MEG)
EditorsF. Martin, S. Uroz
Place of PublicationNew York
Number of pages18
ISBN (Electronic)9781071628713
ISBN (Print)9781071628706
Publication statusPublished - 16 Dec 2022

Publication series

NameMethods in molecular biology
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029


  • Carbon thirteen
  • Carbon transfer
  • Fungal plant symbiont
  • qRT-PCR
  • RNA
  • Stable-isotope probing (SIP)


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