Stability of (Bio)Functionalized Porous Aluminum Oxide

A. Debrassi, A. Ribbera, W.M. de Vos, T. Wennekes, H. Zuilhof

Research output: Contribution to journalArticleAcademicpeer-review

35 Citations (Scopus)


Porous aluminum oxide (PAO), a nanostructured support for, among others, culturing microorganisms, was chemically modified in order to attach biomolecules that can selectively interact with target bacteria. We present the first comprehensive study of monolayer-modified PAO using conditions that are relevant to microbial growth with a range of functional groups (carboxylic acid, a-hydroxycarboxylic acid, alkyne, alkene, phosphonic acid, and silane). Their stability was initially assessed in phosphate-buffered saline (pH 7.0) at room temperature. The most stable combination (PAO with phosphonic acids) was further studied over a range of physiological pHs (4–8) and temperatures (up to 80 °C). Varying the pH had no significant effect on the stability, but it gradually decreased with increasing temperature. The stability of phosphonic acid-modified PAO surfaces was shown to depend strongly on the other terminal group of the monolayer structure: in general, hydrophilic monolayers were less stable than hydrophobic monolayers. Finally, an alkyne-terminated PAO surface was reacted with an azide-linked mannose derivative. The resulting mannose-presenting PAO surface showed the clearly increased adherence of a mannose-binding bacterium, Lactobacillus plantarum, and also allowed for bacterial outgrowth.
Original languageEnglish
Pages (from-to)1311-1320
Publication statusPublished - 2014


  • self-assembled monolayers
  • lactic-acid bacteria
  • lactobacillus-plantarum
  • nanoporous alumina
  • click chemistry
  • anodic alumina
  • surfaces
  • adsorption
  • membrane
  • carbohydrate


Dive into the research topics of 'Stability of (Bio)Functionalized Porous Aluminum Oxide'. Together they form a unique fingerprint.

Cite this