Spreeta-based biosensor immunoassays to detect fraudulent adulteration in milk and milk powder

W. Haasnoot, G.R. Marchesini, K. Koopal

    Research output: Contribution to journalArticleAcademicpeer-review

    25 Citations (Scopus)


    Biacore biosensors (Biacore AB, Uppsala, Sweden) have proven to be robust analytical tools for the automated immunochemical detection of different adulterants and contaminants in milk and milk powder. However, the significant cost of the instruments is a disincentive for their wide application in food control laboratories. Therefore, a low-cost alternative optical biosensor (Spreeta¿, Texas Instruments, Attleboro, MA) was built into an affordable liquid handling system. Using this prototype biosensor, an inhibition immunoassay for bovine ¿-casein was evaluated for the detection of cow's milk in ewe's and goat's milk and for the detection of bovine rennet whey powder in milk powder. Comparable sensitivities were obtained for both adulterants in the Spreeta-based prototype biosensor and a Biacore 3000 instrument. The limit of detection for cow's milk was 0.17% (v/v) and bovine rennet whey powder could be detected in milk powder above 1% (w/w). The Spreeta sensor was also useful in the control of fraudulent water additions to milk, simply by measuring differences in the bulk response
    Original languageEnglish
    Pages (from-to)849-855
    JournalJournal of AOAC International
    Issue number3
    Publication statusPublished - 2006


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