Sponge holobionts shift their prokaryotic communities and antimicrobial activity from shallow to lower mesophotic depths

Anak Agung Gede Indraningrat, Georg Steinert, Leontine E. Becking, Benjamin Mueller, Jasper M. de Goeij, Hauke Smidt, Detmer Sipkema*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

3 Citations (Scopus)

Abstract

In this study, we used 16S rRNA gene amplicon sequencing to investigate prokaryotic community composition of the Caribbean sponges Xestospongia muta and Agelas sventres from three depth ranges: < 30 m (shallow), 30–60 m (upper mesophotic), and 60–90 m (lower mesophotic). The prokaryotic community in shallow samples of X. muta was enriched in Cyanobacteria, Chloroflexota, and Crenarchaeota compared to samples from mesophotic depths, while mesophotic samples of X. muta were enriched in Acidobacteriota. For A. sventres, relative abundance of Acidobacteriota, Chloroflexota, and Gammaproteobacteria was higher in shallow samples, while Proteobacteria and Crenarchaeota were enriched in mesophotic A. sventres samples. Antimicrobial activity was evaluated by screening crude extracts of sponges against a set of Gram-positive and Gram-negative bacteria, a yeast, and an oomycete. Antibacterial activities from crude extracts of shallow sponge individuals were generally higher than observed from mesophotic individuals, that showed limited or no antibacterial activities. Conversely, the highest anti-oomycete activity was found from crude extracts of X. muta individuals from lower mesophotic depth, but without a clear pattern across the depth gradient. These results indicate that sponge-associated prokaryotic communities and the antimicrobial activity of sponges change within species across a depth gradient from shallow to mesophotic depth.

Original languageEnglish
Pages (from-to)1265-1283
JournalAntonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Volume115
Issue number10
Early online date23 Aug 2022
DOIs
Publication statusPublished - Oct 2022

Keywords

  • Antimicrobial activity
  • Depth
  • Prokaryotic community
  • Sponges

Fingerprint

Dive into the research topics of 'Sponge holobionts shift their prokaryotic communities and antimicrobial activity from shallow to lower mesophotic depths'. Together they form a unique fingerprint.

Cite this