Sponge cell culture? A molecular identification method for sponge cells

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Abstract

Dissociated sponge cells are easily confused with unicellular organisms. This has been an obstacle in the development of sponge-cell lines. We developed a molecular detection method to identify cells of the sponge Dysidea avara in dissociated cell cultures. The 18S ribosomal RNA gene from a Dysidea avara specimen was sequenced and compared to eukaryotic 18S rDNA sequences picked up from a proliferating cell culture that originated from a dissociated Dysidea avara specimen. Our method proved unambiguously that this was not a sponge-cell culture. Therefore, it provides a valuable tool for further research on sponge-cell cultures
Original languageEnglish
Pages (from-to)443-449
JournalMarine Biotechnology
Volume5
Issue number5
DOIs
Publication statusPublished - 2003

Keywords

  • marine sponges
  • cultivation

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