Abstract
Spodoptera exigua multiple nucleopolyhedrovirus (SeMNPV) and Agrotis segetum
NPV are close relatives, but distinct baculovirus species. Their genomic organization is
remarkably similar. The cross infectivity of these two viruses for S. exigua and A. segetum larvae
has been analyzed. AgseNPV was able to infect S. exigua orally, although the dose needed was
high (LD50 of 8,3 x 105 OBs/larvae) and the yield of progeny polyhedra very low. In contrast,
SeMNPV appeared to be non-infectious for A. segetum larvae when administered per os. The
course of SeMNPV infection in A. segetum was monitored by real-time PCR in a time course
experiment and showed that SeMNPV was able to infect and produce polyhedra in A. segetum
larvae by intrahemocoelic injection. Infection of A. segetum with a mixture of AgseNPV and
SeMNPV, only resulted in AgseNPV polyhedra. So, in the case of SeMNPV the midgut is the
major barrier for infection. The possible mechanisms of A. segetum resistance for SeMNPV per
os are discussed.
| Original language | English |
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| Title of host publication | Abstract Book of the 12th European Meeting of the IOBC/WPRS Working Groups on "Insect Pathogens and Insect Parasitic Nematodes", Pamplona, Spain, 22 - 25 Jue, 2009 |
| Place of Publication | Pamplona |
| Pages | 4-4 |
| Publication status | Published - 2009 |
| Event | 12th European Meeting of the IOBC/WPRS Working Gorup on "Insect Pathogens and Insect Parasitic Nematodes", Pamplona, Spain - Duration: 22 Jun 2009 → 25 Jun 2009 |
Conference/symposium
| Conference/symposium | 12th European Meeting of the IOBC/WPRS Working Gorup on "Insect Pathogens and Insect Parasitic Nematodes", Pamplona, Spain |
|---|---|
| Period | 22/06/09 → 25/06/09 |