Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 87-92 |
| Journal | Graefes Archive for Clinical and Experimental Ophthalmology |
| Volume | 247 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 2009 |
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Keywords
- antigen-presenting cells
- tgf-beta
- regulatory cells
- ifn-gamma
- in-vitro
- interferon-gamma
- cutting edge
- induction
- foxp3
- responses
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Splenic CD8(+) T cells secrete TGF-beta 1 to exert suppression in mice with anterior chamber-associated immune deviation. / Jiang, L.Q.; He, H.; Yang, P.Z.; Lin, X.M.; Zhou, H.Y.; Huang, X.K.; Kijlstra, A.
In: Graefes Archive for Clinical and Experimental Ophthalmology, Vol. 247, No. 1, 2009, p. 87-92.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Splenic CD8(+) T cells secrete TGF-beta 1 to exert suppression in mice with anterior chamber-associated immune deviation
AU - Jiang, L.Q.
AU - He, H.
AU - Yang, P.Z.
AU - Lin, X.M.
AU - Zhou, H.Y.
AU - Huang, X.K.
AU - Kijlstra, A.
N1 - ISI:000261750600011
PY - 2009
Y1 - 2009
N2 - Background CD8(+) regulatory T cells (Treg) have been considered to be involved in a model of ocular-induced tolerance, known as anterior chamber-associated immune deviation (ACAID). The mechanisms of suppression by CD8(+) T cells in ACAID remain only poorly understood. TGF-beta 1 is considered as an inhibitory cytokine for immunosuppression in some models. The production of TGF-beta 1 by CD8(+) T cells in ACAID, and whether CD8+ T cells exert suppression through TGF-beta 1, is unknown. Methods The suppressive effect of CD8(+) T cells in ACAID mice was determined by a local adoptive transfer (LAT) assay. The production of TGF-beta 1 by CD8(+) T cells was measured by enzyme-linked immunosorbent assay (ELISA). Anti-TGF-beta 1 antibodies were used in the LAT assay to test if they could block the inhibitory effect of CD8(+) T cells. Results CD8(+) T cells from ACAID mice were shown to block the delayed-type hypersensitivity (DTH) response in an antigen-specific manner in a LAT assay. These CD8+ T cells secreted TGF-beta 1, and their suppression could partially be blocked by anti-TGF-beta 1 antibodies. Conclusions Our study confirms that CD8+ T cells from ACAID mice possess inhibitory properties. This population exerts part of its suppressive function via the production of TGF-beta 1.
AB - Background CD8(+) regulatory T cells (Treg) have been considered to be involved in a model of ocular-induced tolerance, known as anterior chamber-associated immune deviation (ACAID). The mechanisms of suppression by CD8(+) T cells in ACAID remain only poorly understood. TGF-beta 1 is considered as an inhibitory cytokine for immunosuppression in some models. The production of TGF-beta 1 by CD8(+) T cells in ACAID, and whether CD8+ T cells exert suppression through TGF-beta 1, is unknown. Methods The suppressive effect of CD8(+) T cells in ACAID mice was determined by a local adoptive transfer (LAT) assay. The production of TGF-beta 1 by CD8(+) T cells was measured by enzyme-linked immunosorbent assay (ELISA). Anti-TGF-beta 1 antibodies were used in the LAT assay to test if they could block the inhibitory effect of CD8(+) T cells. Results CD8(+) T cells from ACAID mice were shown to block the delayed-type hypersensitivity (DTH) response in an antigen-specific manner in a LAT assay. These CD8+ T cells secreted TGF-beta 1, and their suppression could partially be blocked by anti-TGF-beta 1 antibodies. Conclusions Our study confirms that CD8+ T cells from ACAID mice possess inhibitory properties. This population exerts part of its suppressive function via the production of TGF-beta 1.
KW - antigen-presenting cells
KW - tgf-beta
KW - regulatory cells
KW - ifn-gamma
KW - in-vitro
KW - interferon-gamma
KW - cutting edge
KW - induction
KW - foxp3
KW - responses
U2 - 10.1007/s00417-008-0947-8
DO - 10.1007/s00417-008-0947-8
M3 - Article
VL - 247
SP - 87
EP - 92
JO - Graefes Archive for Clinical and Experimental Ophthalmology
JF - Graefes Archive for Clinical and Experimental Ophthalmology
SN - 0721-832X
IS - 1
ER -