Splenic CD8(+) T cells secrete TGF-beta 1 to exert suppression in mice with anterior chamber-associated immune deviation

L.Q. Jiang, H. He, P.Z. Yang, X.M. Lin, H.Y. Zhou, X.K. Huang, A. Kijlstra

Research output: Contribution to journalArticleAcademicpeer-review

11 Citations (Scopus)

Abstract

Background CD8(+) regulatory T cells (Treg) have been considered to be involved in a model of ocular-induced tolerance, known as anterior chamber-associated immune deviation (ACAID). The mechanisms of suppression by CD8(+) T cells in ACAID remain only poorly understood. TGF-beta 1 is considered as an inhibitory cytokine for immunosuppression in some models. The production of TGF-beta 1 by CD8(+) T cells in ACAID, and whether CD8+ T cells exert suppression through TGF-beta 1, is unknown. Methods The suppressive effect of CD8(+) T cells in ACAID mice was determined by a local adoptive transfer (LAT) assay. The production of TGF-beta 1 by CD8(+) T cells was measured by enzyme-linked immunosorbent assay (ELISA). Anti-TGF-beta 1 antibodies were used in the LAT assay to test if they could block the inhibitory effect of CD8(+) T cells. Results CD8(+) T cells from ACAID mice were shown to block the delayed-type hypersensitivity (DTH) response in an antigen-specific manner in a LAT assay. These CD8+ T cells secreted TGF-beta 1, and their suppression could partially be blocked by anti-TGF-beta 1 antibodies. Conclusions Our study confirms that CD8+ T cells from ACAID mice possess inhibitory properties. This population exerts part of its suppressive function via the production of TGF-beta 1.
Original languageEnglish
Pages (from-to)87-92
JournalGraefes Archive for Clinical and Experimental Ophthalmology
Volume247
Issue number1
DOIs
Publication statusPublished - 2009

Keywords

  • antigen-presenting cells
  • tgf-beta
  • regulatory cells
  • ifn-gamma
  • in-vitro
  • interferon-gamma
  • cutting edge
  • induction
  • foxp3
  • responses

Fingerprint Dive into the research topics of 'Splenic CD8(+) T cells secrete TGF-beta 1 to exert suppression in mice with anterior chamber-associated immune deviation'. Together they form a unique fingerprint.

Cite this