Splenic CD8(+) T cells secrete TGF-beta 1 to exert suppression in mice with anterior chamber-associated immune deviation

L.Q. Jiang, H. He, P.Z. Yang, X.M. Lin, H.Y. Zhou, X.K. Huang, A. Kijlstra

Research output: Contribution to journalArticleAcademicpeer-review

11 Citations (Scopus)

Abstract

Background CD8(+) regulatory T cells (Treg) have been considered to be involved in a model of ocular-induced tolerance, known as anterior chamber-associated immune deviation (ACAID). The mechanisms of suppression by CD8(+) T cells in ACAID remain only poorly understood. TGF-beta 1 is considered as an inhibitory cytokine for immunosuppression in some models. The production of TGF-beta 1 by CD8(+) T cells in ACAID, and whether CD8+ T cells exert suppression through TGF-beta 1, is unknown. Methods The suppressive effect of CD8(+) T cells in ACAID mice was determined by a local adoptive transfer (LAT) assay. The production of TGF-beta 1 by CD8(+) T cells was measured by enzyme-linked immunosorbent assay (ELISA). Anti-TGF-beta 1 antibodies were used in the LAT assay to test if they could block the inhibitory effect of CD8(+) T cells. Results CD8(+) T cells from ACAID mice were shown to block the delayed-type hypersensitivity (DTH) response in an antigen-specific manner in a LAT assay. These CD8+ T cells secreted TGF-beta 1, and their suppression could partially be blocked by anti-TGF-beta 1 antibodies. Conclusions Our study confirms that CD8+ T cells from ACAID mice possess inhibitory properties. This population exerts part of its suppressive function via the production of TGF-beta 1.
Original languageEnglish
Pages (from-to)87-92
JournalGraefes Archive for Clinical and Experimental Ophthalmology
Volume247
Issue number1
DOIs
Publication statusPublished - 2009

Fingerprint

Transforming Growth Factor beta1
Anterior Chamber
T-Lymphocytes
Adoptive Transfer
Antibodies
Delayed Hypersensitivity
Regulatory T-Lymphocytes
Immunosuppression
Enzyme-Linked Immunosorbent Assay
Cytokines
Antigens

Keywords

  • antigen-presenting cells
  • tgf-beta
  • regulatory cells
  • ifn-gamma
  • in-vitro
  • interferon-gamma
  • cutting edge
  • induction
  • foxp3
  • responses

Cite this

Jiang, L.Q. ; He, H. ; Yang, P.Z. ; Lin, X.M. ; Zhou, H.Y. ; Huang, X.K. ; Kijlstra, A. / Splenic CD8(+) T cells secrete TGF-beta 1 to exert suppression in mice with anterior chamber-associated immune deviation. In: Graefes Archive for Clinical and Experimental Ophthalmology. 2009 ; Vol. 247, No. 1. pp. 87-92.
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title = "Splenic CD8(+) T cells secrete TGF-beta 1 to exert suppression in mice with anterior chamber-associated immune deviation",
abstract = "Background CD8(+) regulatory T cells (Treg) have been considered to be involved in a model of ocular-induced tolerance, known as anterior chamber-associated immune deviation (ACAID). The mechanisms of suppression by CD8(+) T cells in ACAID remain only poorly understood. TGF-beta 1 is considered as an inhibitory cytokine for immunosuppression in some models. The production of TGF-beta 1 by CD8(+) T cells in ACAID, and whether CD8+ T cells exert suppression through TGF-beta 1, is unknown. Methods The suppressive effect of CD8(+) T cells in ACAID mice was determined by a local adoptive transfer (LAT) assay. The production of TGF-beta 1 by CD8(+) T cells was measured by enzyme-linked immunosorbent assay (ELISA). Anti-TGF-beta 1 antibodies were used in the LAT assay to test if they could block the inhibitory effect of CD8(+) T cells. Results CD8(+) T cells from ACAID mice were shown to block the delayed-type hypersensitivity (DTH) response in an antigen-specific manner in a LAT assay. These CD8+ T cells secreted TGF-beta 1, and their suppression could partially be blocked by anti-TGF-beta 1 antibodies. Conclusions Our study confirms that CD8+ T cells from ACAID mice possess inhibitory properties. This population exerts part of its suppressive function via the production of TGF-beta 1.",
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Splenic CD8(+) T cells secrete TGF-beta 1 to exert suppression in mice with anterior chamber-associated immune deviation. / Jiang, L.Q.; He, H.; Yang, P.Z.; Lin, X.M.; Zhou, H.Y.; Huang, X.K.; Kijlstra, A.

In: Graefes Archive for Clinical and Experimental Ophthalmology, Vol. 247, No. 1, 2009, p. 87-92.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Splenic CD8(+) T cells secrete TGF-beta 1 to exert suppression in mice with anterior chamber-associated immune deviation

AU - Jiang, L.Q.

AU - He, H.

AU - Yang, P.Z.

AU - Lin, X.M.

AU - Zhou, H.Y.

AU - Huang, X.K.

AU - Kijlstra, A.

N1 - ISI:000261750600011

PY - 2009

Y1 - 2009

N2 - Background CD8(+) regulatory T cells (Treg) have been considered to be involved in a model of ocular-induced tolerance, known as anterior chamber-associated immune deviation (ACAID). The mechanisms of suppression by CD8(+) T cells in ACAID remain only poorly understood. TGF-beta 1 is considered as an inhibitory cytokine for immunosuppression in some models. The production of TGF-beta 1 by CD8(+) T cells in ACAID, and whether CD8+ T cells exert suppression through TGF-beta 1, is unknown. Methods The suppressive effect of CD8(+) T cells in ACAID mice was determined by a local adoptive transfer (LAT) assay. The production of TGF-beta 1 by CD8(+) T cells was measured by enzyme-linked immunosorbent assay (ELISA). Anti-TGF-beta 1 antibodies were used in the LAT assay to test if they could block the inhibitory effect of CD8(+) T cells. Results CD8(+) T cells from ACAID mice were shown to block the delayed-type hypersensitivity (DTH) response in an antigen-specific manner in a LAT assay. These CD8+ T cells secreted TGF-beta 1, and their suppression could partially be blocked by anti-TGF-beta 1 antibodies. Conclusions Our study confirms that CD8+ T cells from ACAID mice possess inhibitory properties. This population exerts part of its suppressive function via the production of TGF-beta 1.

AB - Background CD8(+) regulatory T cells (Treg) have been considered to be involved in a model of ocular-induced tolerance, known as anterior chamber-associated immune deviation (ACAID). The mechanisms of suppression by CD8(+) T cells in ACAID remain only poorly understood. TGF-beta 1 is considered as an inhibitory cytokine for immunosuppression in some models. The production of TGF-beta 1 by CD8(+) T cells in ACAID, and whether CD8+ T cells exert suppression through TGF-beta 1, is unknown. Methods The suppressive effect of CD8(+) T cells in ACAID mice was determined by a local adoptive transfer (LAT) assay. The production of TGF-beta 1 by CD8(+) T cells was measured by enzyme-linked immunosorbent assay (ELISA). Anti-TGF-beta 1 antibodies were used in the LAT assay to test if they could block the inhibitory effect of CD8(+) T cells. Results CD8(+) T cells from ACAID mice were shown to block the delayed-type hypersensitivity (DTH) response in an antigen-specific manner in a LAT assay. These CD8+ T cells secreted TGF-beta 1, and their suppression could partially be blocked by anti-TGF-beta 1 antibodies. Conclusions Our study confirms that CD8+ T cells from ACAID mice possess inhibitory properties. This population exerts part of its suppressive function via the production of TGF-beta 1.

KW - antigen-presenting cells

KW - tgf-beta

KW - regulatory cells

KW - ifn-gamma

KW - in-vitro

KW - interferon-gamma

KW - cutting edge

KW - induction

KW - foxp3

KW - responses

U2 - 10.1007/s00417-008-0947-8

DO - 10.1007/s00417-008-0947-8

M3 - Article

VL - 247

SP - 87

EP - 92

JO - Graefes Archive for Clinical and Experimental Ophthalmology

JF - Graefes Archive for Clinical and Experimental Ophthalmology

SN - 0721-832X

IS - 1

ER -