Spectrin-like proteins in plant nuclei

N.C.A. de Ruijter, T. Ketelaar, S.S.D. Blumenthal, A.M.C. Emons, J.H.N. Schel

Research output: Contribution to journalArticleAcademicpeer-review

18 Citations (Scopus)

Abstract

We analysed the presence and localization of spectrin-like proteins in nuclei of various plant tissues, using several anti-erythrocyte spectrin antibodies on isolated pea nuclei and nuclei in cells. Western blots of extracted purified pea nuclei show a cross-reactive pair of bands at 220–240 kDa, typical for human erythrocyte spectrin, and a prominent 60 kDa band. Immunolocalization by means of confocal laser scanning microscopy reveals spectrin-like proteins in distinct spots equally distributed in the nucleoplasm and over the nuclear periphery, independent of the origin of the anti-spectrin antibodies used. In some nuclei tracks of spectrin-like proteins are also observed. No signal is present in nucleoli. The amount and intensity of signal increases when nuclei were extracted, successively, with detergents, DNase I and RNase A, and high salt, indicating that the spectrin-like protein is associated with the nuclear matrix. The labelling is similar in nuclei of various plant tissues. These data are the first that show the presence and localization of spectrin-like epitopes in plant nuclei, where they may stabilize specific interchromatin domains.
Original languageEnglish
Pages (from-to)427-438
JournalCell Biology International
Volume24
DOIs
Publication statusPublished - 2000

Fingerprint Dive into the research topics of 'Spectrin-like proteins in plant nuclei'. Together they form a unique fingerprint.

  • Cite this