Specific-immuno-(chymo) trypsin-inhibitor-assays for determination of (residual) activity of Bowman-Birk or Kunitz soyabean trypsin inhibitors

A. van Amerongen; V. Ostafe; H. Gruppen; G. Meerdink; L.B.J.M. Berendsen; M. Koets; J.H. Wichers

Specific-immuno-(chymo) trypsin-inhibitor-assays for determination of (residual) activity of Bowman-Birk or Kunitz soyabean trypsin inhibitors

A. van Amerongen, V. Ostafe, H. Gruppen, G. Meerdink, L.B.J.M. Berendsen, M. Koets, J.H. Wichers

Research output: Chapter in Book/Report/Conference proceeding › Conference paper › Academic

Abstract

Proteinaceous protease inhibitors are the major antinutritional factors in soyabeans. Accurate measurement of the protease inhibitor activity is very important in soyabean processing to yield a high-quality product for human and animal nutrition. A test principle that meets these characteristics is described. The principle is based on the binding between enzyme and functional inhibitor followed by the sensitive and accurate determination of the bound inhibitor by specific antibodies. Methods based on this principle can be performed in microtitre plates. Data obtained compare well with those from conventional trypsin inhibitor assays. The most interesting feature is the possibility of assessing the specific characteristics and properties of the different inhibitors, Kunitz soyabean trypsin inhibitor and Bowman-Birk inhibitor, in response to a variety of conditions, such as heat treatment and extrusion cooking. In this way, the inactivation kinetics of individual trypsin inhibitors (classes or iso-inhibitors) can be easily be determined.

Original language	English
Title of host publication	Recent advances of research in antinutritional factors in legume seeds and rapeseed
Subtitle of host publication	Proceedings of the Third International Workshop on Antinutritional Factors in Legume Seeds and Rapeseed
Editors	A.J.M. Jansman, G.D. Hill, J. Huisman, A.F.B. van der Poel
Publisher	Wageningen Pers
Pages	33-37
Publication status	Published - 30 Oct 1998
Event	Third International Workshop on Antinutritional Factors in Legume Seeds and Rapeseed - Wageningen, Netherlands Duration: 8 Jul 1998 → 10 Jul 1998

Workshop

Workshop	Third International Workshop on Antinutritional Factors in Legume Seeds and Rapeseed
Country/Territory	Netherlands
City	Wageningen
Period	8/07/98 → 10/07/98

Cite this

van Amerongen, A., Ostafe, V., Gruppen, H., Meerdink, G., Berendsen, L. B. J. M., Koets, M., & Wichers, J. H. (1998). Specific-immuno-(chymo) trypsin-inhibitor-assays for determination of (residual) activity of Bowman-Birk or Kunitz soyabean trypsin inhibitors. In A. J. M. Jansman, G. D. Hill, J. Huisman, & A. F. B. van der Poel (Eds.), Recent advances of research in antinutritional factors in legume seeds and rapeseed: Proceedings of the Third International Workshop on Antinutritional Factors in Legume Seeds and Rapeseed (pp. 33-37). Wageningen Pers.

van Amerongen, A. ; Ostafe, V. ; Gruppen, H. et al. / Specific-immuno-(chymo) trypsin-inhibitor-assays for determination of (residual) activity of Bowman-Birk or Kunitz soyabean trypsin inhibitors. Recent advances of research in antinutritional factors in legume seeds and rapeseed: Proceedings of the Third International Workshop on Antinutritional Factors in Legume Seeds and Rapeseed. editor / A.J.M. Jansman ; G.D. Hill ; J. Huisman ; A.F.B. van der Poel. Wageningen Pers, 1998. pp. 33-37

@inproceedings{59b32516135540aba816a907c3ebb2e5,

title = "Specific-immuno-(chymo) trypsin-inhibitor-assays for determination of (residual) activity of Bowman-Birk or Kunitz soyabean trypsin inhibitors",

abstract = "Proteinaceous protease inhibitors are the major antinutritional factors in soyabeans. Accurate measurement of the protease inhibitor activity is very important in soyabean processing to yield a high-quality product for human and animal nutrition. A test principle that meets these characteristics is described. The principle is based on the binding between enzyme and functional inhibitor followed by the sensitive and accurate determination of the bound inhibitor by specific antibodies. Methods based on this principle can be performed in microtitre plates. Data obtained compare well with those from conventional trypsin inhibitor assays. The most interesting feature is the possibility of assessing the specific characteristics and properties of the different inhibitors, Kunitz soyabean trypsin inhibitor and Bowman-Birk inhibitor, in response to a variety of conditions, such as heat treatment and extrusion cooking. In this way, the inactivation kinetics of individual trypsin inhibitors (classes or iso-inhibitors) can be easily be determined.",

author = "\{van Amerongen\}, A. and V. Ostafe and H. Gruppen and G. Meerdink and L.B.J.M. Berendsen and M. Koets and J.H. Wichers",

year = "1998",

month = oct,

day = "30",

language = "English",

pages = "33--37",

editor = "Jansman, \{ A.J.M. \} and G.D. Hill and Huisman, \{ J.\} and \{van der Poel\}, \{A.F.B. \}",

booktitle = "Recent advances of research in antinutritional factors in legume seeds and rapeseed",

publisher = "Wageningen Pers",

address = "Netherlands",

note = "Third International Workshop on Antinutritional Factors in Legume Seeds and Rapeseed ; Conference date: 08-07-1998 Through 10-07-1998",

}

van Amerongen, A, Ostafe, V, Gruppen, H, Meerdink, G, Berendsen, LBJM, Koets, M & Wichers, JH 1998, Specific-immuno-(chymo) trypsin-inhibitor-assays for determination of (residual) activity of Bowman-Birk or Kunitz soyabean trypsin inhibitors. in AJM Jansman, GD Hill, J Huisman & AFB van der Poel (eds), Recent advances of research in antinutritional factors in legume seeds and rapeseed: Proceedings of the Third International Workshop on Antinutritional Factors in Legume Seeds and Rapeseed. Wageningen Pers, pp. 33-37, Third International Workshop on Antinutritional Factors in Legume Seeds and Rapeseed, Wageningen, Netherlands, 8/07/98.

Specific-immuno-(chymo) trypsin-inhibitor-assays for determination of (residual) activity of Bowman-Birk or Kunitz soyabean trypsin inhibitors. / van Amerongen, A.; Ostafe, V.; Gruppen, H. et al.
Recent advances of research in antinutritional factors in legume seeds and rapeseed: Proceedings of the Third International Workshop on Antinutritional Factors in Legume Seeds and Rapeseed. ed. / A.J.M. Jansman; G.D. Hill; J. Huisman; A.F.B. van der Poel. Wageningen Pers, 1998. p. 33-37.

Research output: Chapter in Book/Report/Conference proceeding › Conference paper › Academic

TY - GEN

T1 - Specific-immuno-(chymo) trypsin-inhibitor-assays for determination of (residual) activity of Bowman-Birk or Kunitz soyabean trypsin inhibitors

AU - van Amerongen, A.

AU - Ostafe, V.

AU - Gruppen, H.

AU - Meerdink, G.

AU - Berendsen, L.B.J.M.

AU - Koets, M.

AU - Wichers, J.H.

PY - 1998/10/30

Y1 - 1998/10/30

N2 - Proteinaceous protease inhibitors are the major antinutritional factors in soyabeans. Accurate measurement of the protease inhibitor activity is very important in soyabean processing to yield a high-quality product for human and animal nutrition. A test principle that meets these characteristics is described. The principle is based on the binding between enzyme and functional inhibitor followed by the sensitive and accurate determination of the bound inhibitor by specific antibodies. Methods based on this principle can be performed in microtitre plates. Data obtained compare well with those from conventional trypsin inhibitor assays. The most interesting feature is the possibility of assessing the specific characteristics and properties of the different inhibitors, Kunitz soyabean trypsin inhibitor and Bowman-Birk inhibitor, in response to a variety of conditions, such as heat treatment and extrusion cooking. In this way, the inactivation kinetics of individual trypsin inhibitors (classes or iso-inhibitors) can be easily be determined.

AB - Proteinaceous protease inhibitors are the major antinutritional factors in soyabeans. Accurate measurement of the protease inhibitor activity is very important in soyabean processing to yield a high-quality product for human and animal nutrition. A test principle that meets these characteristics is described. The principle is based on the binding between enzyme and functional inhibitor followed by the sensitive and accurate determination of the bound inhibitor by specific antibodies. Methods based on this principle can be performed in microtitre plates. Data obtained compare well with those from conventional trypsin inhibitor assays. The most interesting feature is the possibility of assessing the specific characteristics and properties of the different inhibitors, Kunitz soyabean trypsin inhibitor and Bowman-Birk inhibitor, in response to a variety of conditions, such as heat treatment and extrusion cooking. In this way, the inactivation kinetics of individual trypsin inhibitors (classes or iso-inhibitors) can be easily be determined.

M3 - Conference paper

SP - 33

EP - 37

BT - Recent advances of research in antinutritional factors in legume seeds and rapeseed

A2 - Jansman, A.J.M.

A2 - Hill, G.D.

A2 - Huisman, J.

A2 - van der Poel, A.F.B.

PB - Wageningen Pers

T2 - Third International Workshop on Antinutritional Factors in Legume Seeds and Rapeseed

Y2 - 8 July 1998 through 10 July 1998

ER -

van Amerongen A, Ostafe V, Gruppen H, Meerdink G, Berendsen LBJM, Koets M et al. Specific-immuno-(chymo) trypsin-inhibitor-assays for determination of (residual) activity of Bowman-Birk or Kunitz soyabean trypsin inhibitors. In Jansman AJM, Hill GD, Huisman J, van der Poel AFB, editors, Recent advances of research in antinutritional factors in legume seeds and rapeseed: Proceedings of the Third International Workshop on Antinutritional Factors in Legume Seeds and Rapeseed. Wageningen Pers. 1998. p. 33-37

Specific-immuno-(chymo) trypsin-inhibitor-assays for determination of (residual) activity of Bowman-Birk or Kunitz soyabean trypsin inhibitors

Abstract

Workshop

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Cite this