Spatial distributions of nematode taxa in arable and (semi-)natural fields

C.W. Quist, P.J.W. Mooijman, S.J.J. van den Elsen, D.J. Brus, C. Mulder, A. Termoshuizen, J. Bakker, J. Helder

Research output: Chapter in Book/Report/Conference proceedingAbstract

Abstract

Because of their abundance, their trophic diversity and their relatively straightforward extractability, nematodes have a potential as a proxy for the biological condition of soils and sediments. So far the potential of this group is underexploited for technical reasons; the microscopic analysis of nematode assemblages is labour intensive and requires extensive expertise. With the availability of a relatively large molecular framework, it became possible to design molecular assays that allow for the quantitative analysis of individual taxa against complex DNA backgrounds. The availability of high throughput tools to monitor nematode assemblages makes it possible to investigate the spatial distribution of nematode taxa at field level. Experts are aware that some nematodes occur in patches whereas others are more or less evenly distributed over a field. In order to assess the 6th International Congress of Nematology 155 degree of patchiness of individual nematode taxa, twelve fields (4 from each marine clay, river clay and sandy soils) of a single hectare each were investigated in great detail. Using a sampling grid optimized for geostatistic analysis, 96 composite samples were taken from each of these fields. Each of these samples were analysed with 24 molecular assays (corresponding to 23 nematode taxa and one internal control). Preliminary results, visualized as semi-variograms and surface maps, show a great variation in distribution patterns not only on the basis of nematode taxa but also based on soil type. The data that will be presented are essential for the design of scientifically sound sampling schemes for agricultural and natural soil at hectare scale and above.
Original languageEnglish
Title of host publicationProceedings of the 6th International Congress of Nematology
Pages154-155
Publication statusPublished - 2014
Event6th International Congress of Nematology, Cape Town, South Africa -
Duration: 4 May 20149 May 2014

Conference

Conference6th International Congress of Nematology, Cape Town, South Africa
Period4/05/149/05/14

Fingerprint

nematode
spatial distribution
assay
patchiness
geostatistics
variogram
sampling
clay soil
sandy soil
quantitative analysis
soil type
soil
labor
DNA
clay
river
sediment

Cite this

Quist, C. W., Mooijman, P. J. W., van den Elsen, S. J. J., Brus, D. J., Mulder, C., Termoshuizen, A., ... Helder, J. (2014). Spatial distributions of nematode taxa in arable and (semi-)natural fields. In Proceedings of the 6th International Congress of Nematology (pp. 154-155)
Quist, C.W. ; Mooijman, P.J.W. ; van den Elsen, S.J.J. ; Brus, D.J. ; Mulder, C. ; Termoshuizen, A. ; Bakker, J. ; Helder, J. / Spatial distributions of nematode taxa in arable and (semi-)natural fields. Proceedings of the 6th International Congress of Nematology. 2014. pp. 154-155
@inbook{c23183aaca8f49aebe6c6f18049028d5,
title = "Spatial distributions of nematode taxa in arable and (semi-)natural fields",
abstract = "Because of their abundance, their trophic diversity and their relatively straightforward extractability, nematodes have a potential as a proxy for the biological condition of soils and sediments. So far the potential of this group is underexploited for technical reasons; the microscopic analysis of nematode assemblages is labour intensive and requires extensive expertise. With the availability of a relatively large molecular framework, it became possible to design molecular assays that allow for the quantitative analysis of individual taxa against complex DNA backgrounds. The availability of high throughput tools to monitor nematode assemblages makes it possible to investigate the spatial distribution of nematode taxa at field level. Experts are aware that some nematodes occur in patches whereas others are more or less evenly distributed over a field. In order to assess the 6th International Congress of Nematology 155 degree of patchiness of individual nematode taxa, twelve fields (4 from each marine clay, river clay and sandy soils) of a single hectare each were investigated in great detail. Using a sampling grid optimized for geostatistic analysis, 96 composite samples were taken from each of these fields. Each of these samples were analysed with 24 molecular assays (corresponding to 23 nematode taxa and one internal control). Preliminary results, visualized as semi-variograms and surface maps, show a great variation in distribution patterns not only on the basis of nematode taxa but also based on soil type. The data that will be presented are essential for the design of scientifically sound sampling schemes for agricultural and natural soil at hectare scale and above.",
author = "C.W. Quist and P.J.W. Mooijman and {van den Elsen}, S.J.J. and D.J. Brus and C. Mulder and A. Termoshuizen and J. Bakker and J. Helder",
year = "2014",
language = "English",
pages = "154--155",
booktitle = "Proceedings of the 6th International Congress of Nematology",

}

Quist, CW, Mooijman, PJW, van den Elsen, SJJ, Brus, DJ, Mulder, C, Termoshuizen, A, Bakker, J & Helder, J 2014, Spatial distributions of nematode taxa in arable and (semi-)natural fields. in Proceedings of the 6th International Congress of Nematology. pp. 154-155, 6th International Congress of Nematology, Cape Town, South Africa, 4/05/14.

Spatial distributions of nematode taxa in arable and (semi-)natural fields. / Quist, C.W.; Mooijman, P.J.W.; van den Elsen, S.J.J.; Brus, D.J.; Mulder, C.; Termoshuizen, A.; Bakker, J.; Helder, J.

Proceedings of the 6th International Congress of Nematology. 2014. p. 154-155.

Research output: Chapter in Book/Report/Conference proceedingAbstract

TY - CHAP

T1 - Spatial distributions of nematode taxa in arable and (semi-)natural fields

AU - Quist, C.W.

AU - Mooijman, P.J.W.

AU - van den Elsen, S.J.J.

AU - Brus, D.J.

AU - Mulder, C.

AU - Termoshuizen, A.

AU - Bakker, J.

AU - Helder, J.

PY - 2014

Y1 - 2014

N2 - Because of their abundance, their trophic diversity and their relatively straightforward extractability, nematodes have a potential as a proxy for the biological condition of soils and sediments. So far the potential of this group is underexploited for technical reasons; the microscopic analysis of nematode assemblages is labour intensive and requires extensive expertise. With the availability of a relatively large molecular framework, it became possible to design molecular assays that allow for the quantitative analysis of individual taxa against complex DNA backgrounds. The availability of high throughput tools to monitor nematode assemblages makes it possible to investigate the spatial distribution of nematode taxa at field level. Experts are aware that some nematodes occur in patches whereas others are more or less evenly distributed over a field. In order to assess the 6th International Congress of Nematology 155 degree of patchiness of individual nematode taxa, twelve fields (4 from each marine clay, river clay and sandy soils) of a single hectare each were investigated in great detail. Using a sampling grid optimized for geostatistic analysis, 96 composite samples were taken from each of these fields. Each of these samples were analysed with 24 molecular assays (corresponding to 23 nematode taxa and one internal control). Preliminary results, visualized as semi-variograms and surface maps, show a great variation in distribution patterns not only on the basis of nematode taxa but also based on soil type. The data that will be presented are essential for the design of scientifically sound sampling schemes for agricultural and natural soil at hectare scale and above.

AB - Because of their abundance, their trophic diversity and their relatively straightforward extractability, nematodes have a potential as a proxy for the biological condition of soils and sediments. So far the potential of this group is underexploited for technical reasons; the microscopic analysis of nematode assemblages is labour intensive and requires extensive expertise. With the availability of a relatively large molecular framework, it became possible to design molecular assays that allow for the quantitative analysis of individual taxa against complex DNA backgrounds. The availability of high throughput tools to monitor nematode assemblages makes it possible to investigate the spatial distribution of nematode taxa at field level. Experts are aware that some nematodes occur in patches whereas others are more or less evenly distributed over a field. In order to assess the 6th International Congress of Nematology 155 degree of patchiness of individual nematode taxa, twelve fields (4 from each marine clay, river clay and sandy soils) of a single hectare each were investigated in great detail. Using a sampling grid optimized for geostatistic analysis, 96 composite samples were taken from each of these fields. Each of these samples were analysed with 24 molecular assays (corresponding to 23 nematode taxa and one internal control). Preliminary results, visualized as semi-variograms and surface maps, show a great variation in distribution patterns not only on the basis of nematode taxa but also based on soil type. The data that will be presented are essential for the design of scientifically sound sampling schemes for agricultural and natural soil at hectare scale and above.

M3 - Abstract

SP - 154

EP - 155

BT - Proceedings of the 6th International Congress of Nematology

ER -

Quist CW, Mooijman PJW, van den Elsen SJJ, Brus DJ, Mulder C, Termoshuizen A et al. Spatial distributions of nematode taxa in arable and (semi-)natural fields. In Proceedings of the 6th International Congress of Nematology. 2014. p. 154-155