SOMBRERO, BEARSKIN1, and BEARSKIN2 regulate root cap maturation in Arabidopsis

Tom Bennett, Albert van den Toorn, Gabino F. Sanchez-Perez, Ana Campilho, Viola Willemsen, Berend Snel, Ben Scheres*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

93 Citations (Scopus)

Abstract

The root cap has a central role in root growth, determining the growth trajectory and facilitating penetration into the soil. Root cap cells have specialized functions and morphologies, and border cells are released into the rhizosphere by specific cell wall modifications. Here, we demonstrate that the cellular maturation of root cap is redundantly regulated by three genes, SOMBRERO (SMB), BEARSKIN1 (BRN1), and BRN2, which are members of the Class IIB NAC transcription factor family, together with the VASCULAR NAC DOMAIN (VND) and NAC SECONDARY WALL THICKENING PROMOTING FACTOR (NST) genes that regulate secondary cell wall synthesis in specialized cell types. Lateral cap cells in smb-3 mutants continue to divide and fail to detach from the root, phenotypes that are independent of FEZ upregulation in smb-3. In brn1-1 brn2-1 double mutants, columella cells fail to detach, while in triple mutants, cells fail to mature in all parts of the cap. This complex genetic redundancy involves differences in expression, protein activity, and target specificity. All three genes have very similar overexpression phenotypes to the VND/NST genes, indicating that members of this family are largely functionally equivalent. Our results suggest that Class IIB NAC proteins regulate cell maturation in cells that undergo terminal differentiation with strong cell wall modifications.

Original languageEnglish
Pages (from-to)640-654
Number of pages15
JournalPlant Cell
Volume22
Issue number3
DOIs
Publication statusPublished - 1 Mar 2010
Externally publishedYes

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