Somatic embryogenesis in tulip

H. Gude, M.H.G.E. Dijkema

    Research output: Chapter in Book/Report/Conference proceedingConference paperAcademicpeer-review

    20 Citations (Scopus)

    Abstract

    Because the natural propagation rate of tulip is low, modern breeding requires a rapid in vitro propagation method. The best method available at the moment, adventitious shoot formation on stem explants, has a low propagation factor and is laborious, time-consuming and hence expensive. It is the long term aim of the present study to develop a propagation method, consisting of a cell suspension phase, and a regeneration phase, during which the cell clusters regenerate through somatic embryogenesis. A system like this would enable the rapid production of large numbers of bulblets at low costs. In order to obtain friable callus for the start of a cell suspension, bulb scale and stem explants were cultured on a nutrient medium containing the auxins 2,4-D or Picloram at a concentration range from 0.5-50 μM. Undifferentiated and 'meristematic' type of callus on bulb scale explants an were induced. The meristematic type seemed the most suitable to start a liquid culture. In liquid medium the meristematic nodules divided into smaller units, which could be released from the explant by shaking. On stem explants on medium with 5 or 50 μM 2,4-D or Picloram somatic embryos were formed. Morphological and anatomical data on the development of these somatic embryos are presented. The ability to produce somatic embryos offers us the opportunity to use embryo-tissue for the production of embryogenic callus.

    Original languageEnglish
    Title of host publicationActa Horticulturae
    PublisherISHS
    Pages275-280
    Number of pages6
    ISBN (Print)9789066058194
    DOIs
    Publication statusPublished - 1 Dec 1997

    Publication series

    NameActa Horticulturae
    Volume430
    ISSN (Print)0567-7572

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