Somatic embryogenesis from Arabidopsis shoot apical meristem mutants

A.P. Mordhorst; M.V. Hartog; M.K. El Tamer; T. Laux; S.C. de Vries

doi:10.1007/s00425-001-0700-6

Somatic embryogenesis from Arabidopsis shoot apical meristem mutants

A.P. Mordhorst, M.V. Hartog, M.K. El Tamer, T. Laux, S.C. de Vries

Research output: Contribution to journal › Article › Academic › peer-review

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Abstract

Zygotic embryos of three Arabidopsis thaliana (L.) Heynh. mutants lacking an embryonic shoot apical meristem (SAM), shoot meristemless (stm), wuschel (wus) and zwille/pinhead (zll/pnh) were used as explants to establish embryogenic cell cultures. Somatic embryos of all three mutants showed the same mutant phenotypes as their zygotic equivalents. These results provide genetic evidence that the developmental program of somatic and zygotic embryos is indistinguishable. They also suggest that a functional SAM is not required for somatic embryogenic cell formation in Arabidopsis.

Original language	English
Pages (from-to)	829-836
Journal	Planta
Volume	214
Issue number	6
DOIs	https://doi.org/10.1007/s00425-001-0700-6
Publication status	Published - 2002

Keywords

Arabidopsis (embryogenesis)
Developmental program
Embryogenic cell
Shoot apical meristem mutant
Somatic embryogenesis

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10.1007/s00425-001-0700-6

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title = "Somatic embryogenesis from Arabidopsis shoot apical meristem mutants",

abstract = "Zygotic embryos of three Arabidopsis thaliana (L.) Heynh. mutants lacking an embryonic shoot apical meristem (SAM), shoot meristemless (stm), wuschel (wus) and zwille/pinhead (zll/pnh) were used as explants to establish embryogenic cell cultures. Somatic embryos of all three mutants showed the same mutant phenotypes as their zygotic equivalents. These results provide genetic evidence that the developmental program of somatic and zygotic embryos is indistinguishable. They also suggest that a functional SAM is not required for somatic embryogenic cell formation in Arabidopsis.",

keywords = "Arabidopsis (embryogenesis), Developmental program, Embryogenic cell, Shoot apical meristem mutant, Somatic embryogenesis",

author = "A.P. Mordhorst and M.V. Hartog and {El Tamer}, M.K. and T. Laux and {de Vries}, S.C.",

year = "2002",

doi = "10.1007/s00425-001-0700-6",

language = "English",

volume = "214",

pages = "829--836",

journal = "Planta",

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T1 - Somatic embryogenesis from Arabidopsis shoot apical meristem mutants

AU - Mordhorst, A.P.

AU - Hartog, M.V.

AU - El Tamer, M.K.

AU - Laux, T.

AU - de Vries, S.C.

PY - 2002

Y1 - 2002

N2 - Zygotic embryos of three Arabidopsis thaliana (L.) Heynh. mutants lacking an embryonic shoot apical meristem (SAM), shoot meristemless (stm), wuschel (wus) and zwille/pinhead (zll/pnh) were used as explants to establish embryogenic cell cultures. Somatic embryos of all three mutants showed the same mutant phenotypes as their zygotic equivalents. These results provide genetic evidence that the developmental program of somatic and zygotic embryos is indistinguishable. They also suggest that a functional SAM is not required for somatic embryogenic cell formation in Arabidopsis.

AB - Zygotic embryos of three Arabidopsis thaliana (L.) Heynh. mutants lacking an embryonic shoot apical meristem (SAM), shoot meristemless (stm), wuschel (wus) and zwille/pinhead (zll/pnh) were used as explants to establish embryogenic cell cultures. Somatic embryos of all three mutants showed the same mutant phenotypes as their zygotic equivalents. These results provide genetic evidence that the developmental program of somatic and zygotic embryos is indistinguishable. They also suggest that a functional SAM is not required for somatic embryogenic cell formation in Arabidopsis.

KW - Arabidopsis (embryogenesis)

KW - Developmental program

KW - Embryogenic cell

KW - Shoot apical meristem mutant

KW - Somatic embryogenesis

U2 - 10.1007/s00425-001-0700-6

DO - 10.1007/s00425-001-0700-6

M3 - Article

SN - 0032-0935

VL - 214

SP - 829

EP - 836

JO - Planta

JF - Planta

IS - 6

ER -

Somatic embryogenesis from Arabidopsis shoot apical meristem mutants

Abstract

Keywords

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