Solvent exchange module for LC-NMR hyphenation using machine vision-controlled droplet evaporation

J.W. Schoonen, P. Vulto, N. de Roo, J.P.M. van Duynhoven, H. van der Linden, T. Hankemeier

Research output: Contribution to journalArticleAcademicpeer-review

6 Citations (Scopus)

Abstract

We report the use of pendant droplet evaporation for exchange of eluents for 1H nuclear magnetic resonance (1H NMR) purposes. Analytes are fed and retained in 500 nL droplets, which are concentrated by evaporation and subsequently redissolved in deuterated solvent. Droplet size is monitored by machine vision (MV), and heating rates are adjusted concordingly to maintain a stable droplet volume. Evaporation control is independent of solvent properties, and the setup handles feed rates up to 7 µL min–1. The interface is capable of exchanging up to 90% of solvent for deuterated solvent, with a good recovery and repeatability for tomato extracts (Solanum lycopersicum). The system was capable of handling both polar and nonpolar analytes in one run. Volatiles such as formate, acetate, and lactate and the thermosensitive compound epigallocatechin gallate were recovered without significant losses. Ethanol and propionate were recovered with significant losses due to the formation of a minimum boiling azeotrope. The current setup is ideally suited for on- and off-line hyphenation of liquid chromatography and NMR, as it is comprehensive, fully automated, and easy to operate.
Original languageEnglish
Pages (from-to)5734-5739
JournalAnalytical Chemistry
Volume85
Issue number12
DOIs
Publication statusPublished - 2013

Fingerprint

Computer vision
Evaporation
Nuclear magnetic resonance
formic acid
Azeotropes
Liquid chromatography
Propionates
Heating rate
Boiling liquids
Lactic Acid
Acetates
Ethanol
Recovery

Keywords

  • solid-phase extraction
  • nuclear-magnetic-resonance
  • red wine/grape juice
  • ms-spe-nmr
  • natural-products
  • liquid-chromatography
  • flow probe
  • black tea
  • identification
  • system

Cite this

Schoonen, J.W. ; Vulto, P. ; de Roo, N. ; van Duynhoven, J.P.M. ; van der Linden, H. ; Hankemeier, T. / Solvent exchange module for LC-NMR hyphenation using machine vision-controlled droplet evaporation. In: Analytical Chemistry. 2013 ; Vol. 85, No. 12. pp. 5734-5739.
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abstract = "We report the use of pendant droplet evaporation for exchange of eluents for 1H nuclear magnetic resonance (1H NMR) purposes. Analytes are fed and retained in 500 nL droplets, which are concentrated by evaporation and subsequently redissolved in deuterated solvent. Droplet size is monitored by machine vision (MV), and heating rates are adjusted concordingly to maintain a stable droplet volume. Evaporation control is independent of solvent properties, and the setup handles feed rates up to 7 µL min–1. The interface is capable of exchanging up to 90{\%} of solvent for deuterated solvent, with a good recovery and repeatability for tomato extracts (Solanum lycopersicum). The system was capable of handling both polar and nonpolar analytes in one run. Volatiles such as formate, acetate, and lactate and the thermosensitive compound epigallocatechin gallate were recovered without significant losses. Ethanol and propionate were recovered with significant losses due to the formation of a minimum boiling azeotrope. The current setup is ideally suited for on- and off-line hyphenation of liquid chromatography and NMR, as it is comprehensive, fully automated, and easy to operate.",
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author = "J.W. Schoonen and P. Vulto and {de Roo}, N. and {van Duynhoven}, J.P.M. and {van der Linden}, H. and T. Hankemeier",
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Solvent exchange module for LC-NMR hyphenation using machine vision-controlled droplet evaporation. / Schoonen, J.W.; Vulto, P.; de Roo, N.; van Duynhoven, J.P.M.; van der Linden, H.; Hankemeier, T.

In: Analytical Chemistry, Vol. 85, No. 12, 2013, p. 5734-5739.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Solvent exchange module for LC-NMR hyphenation using machine vision-controlled droplet evaporation

AU - Schoonen, J.W.

AU - Vulto, P.

AU - de Roo, N.

AU - van Duynhoven, J.P.M.

AU - van der Linden, H.

AU - Hankemeier, T.

PY - 2013

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N2 - We report the use of pendant droplet evaporation for exchange of eluents for 1H nuclear magnetic resonance (1H NMR) purposes. Analytes are fed and retained in 500 nL droplets, which are concentrated by evaporation and subsequently redissolved in deuterated solvent. Droplet size is monitored by machine vision (MV), and heating rates are adjusted concordingly to maintain a stable droplet volume. Evaporation control is independent of solvent properties, and the setup handles feed rates up to 7 µL min–1. The interface is capable of exchanging up to 90% of solvent for deuterated solvent, with a good recovery and repeatability for tomato extracts (Solanum lycopersicum). The system was capable of handling both polar and nonpolar analytes in one run. Volatiles such as formate, acetate, and lactate and the thermosensitive compound epigallocatechin gallate were recovered without significant losses. Ethanol and propionate were recovered with significant losses due to the formation of a minimum boiling azeotrope. The current setup is ideally suited for on- and off-line hyphenation of liquid chromatography and NMR, as it is comprehensive, fully automated, and easy to operate.

AB - We report the use of pendant droplet evaporation for exchange of eluents for 1H nuclear magnetic resonance (1H NMR) purposes. Analytes are fed and retained in 500 nL droplets, which are concentrated by evaporation and subsequently redissolved in deuterated solvent. Droplet size is monitored by machine vision (MV), and heating rates are adjusted concordingly to maintain a stable droplet volume. Evaporation control is independent of solvent properties, and the setup handles feed rates up to 7 µL min–1. The interface is capable of exchanging up to 90% of solvent for deuterated solvent, with a good recovery and repeatability for tomato extracts (Solanum lycopersicum). The system was capable of handling both polar and nonpolar analytes in one run. Volatiles such as formate, acetate, and lactate and the thermosensitive compound epigallocatechin gallate were recovered without significant losses. Ethanol and propionate were recovered with significant losses due to the formation of a minimum boiling azeotrope. The current setup is ideally suited for on- and off-line hyphenation of liquid chromatography and NMR, as it is comprehensive, fully automated, and easy to operate.

KW - solid-phase extraction

KW - nuclear-magnetic-resonance

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KW - ms-spe-nmr

KW - natural-products

KW - liquid-chromatography

KW - flow probe

KW - black tea

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