Solid-State NMR Spectroscopy for Studying Microtubules and Microtubule-Associated Proteins

Yanzhang Luo, Shengqi Xiang, Alessandra Lucini Paioni, Agnes Adler, Peter Jan Hooikaas, A.S. Jijumon, Carsten Janke, Anna Akhmanova, Marc Baldus*

*Corresponding author for this work

    Research output: Chapter in Book/Report/Conference proceedingChapterAcademicpeer-review

    Abstract

    In this chapter, we describe the preparatory and spectroscopic procedures for conducting solid-state NMR experiments on microtubules (MTs) obtained from human cells and their complexes with microtubule-associated proteins (MAPs). Next to labeling and functional assembly of MTs and MT-MAP complexes, we discuss solid-state NMR approaches, including fast MAS and hyperpolarization methods that can be used to examine these systems. Such studies can provide novel insight into the dynamic properties of MTs and MT-MAP complexes.

    Original languageEnglish
    Title of host publicationStructural Proteomics
    EditorsOwens J.
    PublisherHumana Press Inc.
    Pages193-201
    Number of pages9
    ISBN (Electronic)9781071614068
    ISBN (Print)9781071614051
    DOIs
    Publication statusPublished - 6 May 2021

    Publication series

    NameMethods in Molecular Biology
    Volume2305
    ISSN (Print)1064-3745
    ISSN (Electronic)1940-6029

    Keywords

    • Microtubule-associated proteins
    • Microtubules
    • Protein dynamics
    • Protein interactions
    • Solid-state NMR spectroscopy

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