Soil metagenomics to identify novel mechanisms of antagonism and antifungal activity for the improved control of phytopathogens

L.S. van Overbeek, K. Smalla, M. Bailey, J. Jansson, S. Sjöling, T. Vogel, R. Nalin, J.D. van Elsas

    Research output: Book/ReportReportAcademic

    Abstract

    Objective: This project will explore & exploit the anti-phytopathogen diversity present in selected European suppressive soils representing a wide geographical spread. The untapped functional potential present in the unculturable part of the microbiota of these soils will be accessed using metagenomic cloning of soil DNA. Moreover, as a baseline, antagonistic organisms will also be isolated. Libraries constructed on the basis of the total and the active cell fractions, as well as the isolates, will be screened for anti-phytopathogen function. Selected clones will be as said with respect to the nature of the antagonistic compounds and production loci, involving sequencing. Potential producer strains will be optimised as to gene expression, growth and stability. Based on the gene sequences obtained, molecular tools will be developed and used to monitor soil suppressiveness. The impact (biosafety) of potential applications will be assessed.
    Original languageEnglish
    Place of PublicationWageningen
    PublisherPlant Research International
    Publication statusPublished - 2008

    Fingerprint

    Metagenomics
    Soil
    Microbiota
    Libraries
    Organism Cloning
    Clone Cells
    Gene Expression
    DNA
    Growth
    Genes

    Cite this

    van Overbeek, L. S., Smalla, K., Bailey, M., Jansson, J., Sjöling, S., Vogel, T., ... van Elsas, J. D. (2008). Soil metagenomics to identify novel mechanisms of antagonism and antifungal activity for the improved control of phytopathogens. Wageningen: Plant Research International.
    van Overbeek, L.S. ; Smalla, K. ; Bailey, M. ; Jansson, J. ; Sjöling, S. ; Vogel, T. ; Nalin, R. ; van Elsas, J.D. / Soil metagenomics to identify novel mechanisms of antagonism and antifungal activity for the improved control of phytopathogens. Wageningen : Plant Research International, 2008.
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    title = "Soil metagenomics to identify novel mechanisms of antagonism and antifungal activity for the improved control of phytopathogens",
    abstract = "Objective: This project will explore & exploit the anti-phytopathogen diversity present in selected European suppressive soils representing a wide geographical spread. The untapped functional potential present in the unculturable part of the microbiota of these soils will be accessed using metagenomic cloning of soil DNA. Moreover, as a baseline, antagonistic organisms will also be isolated. Libraries constructed on the basis of the total and the active cell fractions, as well as the isolates, will be screened for anti-phytopathogen function. Selected clones will be as said with respect to the nature of the antagonistic compounds and production loci, involving sequencing. Potential producer strains will be optimised as to gene expression, growth and stability. Based on the gene sequences obtained, molecular tools will be developed and used to monitor soil suppressiveness. The impact (biosafety) of potential applications will be assessed.",
    author = "{van Overbeek}, L.S. and K. Smalla and M. Bailey and J. Jansson and S. Sj{\"o}ling and T. Vogel and R. Nalin and {van Elsas}, J.D.",
    year = "2008",
    language = "English",
    publisher = "Plant Research International",

    }

    van Overbeek, LS, Smalla, K, Bailey, M, Jansson, J, Sjöling, S, Vogel, T, Nalin, R & van Elsas, JD 2008, Soil metagenomics to identify novel mechanisms of antagonism and antifungal activity for the improved control of phytopathogens. Plant Research International, Wageningen.

    Soil metagenomics to identify novel mechanisms of antagonism and antifungal activity for the improved control of phytopathogens. / van Overbeek, L.S.; Smalla, K.; Bailey, M.; Jansson, J.; Sjöling, S.; Vogel, T.; Nalin, R.; van Elsas, J.D.

    Wageningen : Plant Research International, 2008.

    Research output: Book/ReportReportAcademic

    TY - BOOK

    T1 - Soil metagenomics to identify novel mechanisms of antagonism and antifungal activity for the improved control of phytopathogens

    AU - van Overbeek, L.S.

    AU - Smalla, K.

    AU - Bailey, M.

    AU - Jansson, J.

    AU - Sjöling, S.

    AU - Vogel, T.

    AU - Nalin, R.

    AU - van Elsas, J.D.

    PY - 2008

    Y1 - 2008

    N2 - Objective: This project will explore & exploit the anti-phytopathogen diversity present in selected European suppressive soils representing a wide geographical spread. The untapped functional potential present in the unculturable part of the microbiota of these soils will be accessed using metagenomic cloning of soil DNA. Moreover, as a baseline, antagonistic organisms will also be isolated. Libraries constructed on the basis of the total and the active cell fractions, as well as the isolates, will be screened for anti-phytopathogen function. Selected clones will be as said with respect to the nature of the antagonistic compounds and production loci, involving sequencing. Potential producer strains will be optimised as to gene expression, growth and stability. Based on the gene sequences obtained, molecular tools will be developed and used to monitor soil suppressiveness. The impact (biosafety) of potential applications will be assessed.

    AB - Objective: This project will explore & exploit the anti-phytopathogen diversity present in selected European suppressive soils representing a wide geographical spread. The untapped functional potential present in the unculturable part of the microbiota of these soils will be accessed using metagenomic cloning of soil DNA. Moreover, as a baseline, antagonistic organisms will also be isolated. Libraries constructed on the basis of the total and the active cell fractions, as well as the isolates, will be screened for anti-phytopathogen function. Selected clones will be as said with respect to the nature of the antagonistic compounds and production loci, involving sequencing. Potential producer strains will be optimised as to gene expression, growth and stability. Based on the gene sequences obtained, molecular tools will be developed and used to monitor soil suppressiveness. The impact (biosafety) of potential applications will be assessed.

    M3 - Report

    BT - Soil metagenomics to identify novel mechanisms of antagonism and antifungal activity for the improved control of phytopathogens

    PB - Plant Research International

    CY - Wageningen

    ER -

    van Overbeek LS, Smalla K, Bailey M, Jansson J, Sjöling S, Vogel T et al. Soil metagenomics to identify novel mechanisms of antagonism and antifungal activity for the improved control of phytopathogens. Wageningen: Plant Research International, 2008.