Smooth: a statistical method for successful removal of genotyping errors from high-density genetic lilnkage data

H. van Os, P. Stam, R.G.F. Visser, H.J. van Eck

Research output: Contribution to journalArticleAcademicpeer-review

107 Citations (Scopus)

Abstract

High-density genetic linkage maps can be used for purposes such as fine-scale targeted gene cloning and anchoring of physical maps. However, their construction is significantly complicated by even relatively small amounts of scoring errors. Currently available software is not able to solve the ordering ambiguities in marker clusters, which inhibits the application of high-density maps. A statistical method named SMOOTH was developed to remove genotyping errors from genetic linkage data during the mapping process. The program SMOOTH calculates the difference between the observed and predicted values of data points based on data points of neighbouring loci in a given marker order. Highly improbable data points are removed by the program in an iterative process with a mapping algorithm that recalculates the map after cleaning. SMOOTH has been tested with simulated data and experimental mapping data from potato. The simulations prove that this method is able to detect a high amount of scoring errors and demonstrates that the program enables mapping software to successfully construct a very accurate high-density map. In potato the application of the program resulted in a reliable placement of nearly 1,000 markers in one linkage group.
Original languageEnglish
Pages (from-to)187-194
JournalTheoretical and Applied Genetics
Volume112
Issue number1
DOIs
Publication statusPublished - 2005

Keywords

  • maps
  • aflp
  • markers
  • genome

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