Small Subunit Ribosomal DNA-Based Phylogenetic Analysis of Foliar Nematodes (Aphelenchoides spp.) and Their Quantitative Detection in Complex DNA Backgrounds

K.D. Rybarczyk-Mydlowska, P.J.W. Mooijman, H.H.B. van Megen, S.J.J. van den Elsen, M.T.W. Vervoort, P.T.M. Veenhuizen, J. van Doorn, R.H.L. Dees, G. Karssen, J. Bakker, J. Helder

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45 Citations (Scopus)

Abstract

Foliar nematodes, plant-parasitic representatives of the genus Aphelenchoides, constitute a minority in a group dominated by fungivorous species. Distinction between (mostly harmless) fungal feeding Aphelenchoides species and high impact plant parasites such as A. besseyi, A. fragariae, A. ritzemabosi, and A. subtenuis is severely hampered by the scarcity of informative morphological characters, some of which are only observable in specific developmental stages. Poor description of a number of non plant-parasitic Aphelenchoides species further complicates identification. Based on (nearly) full-length small subunit ribosomal DNA (SSU rDNA) sequences (˜ 1,700 bp), a phylogenetic tree was generated, and the four target species appeared as distinct, well-supported groups. Notably, this genus does not constitute a monophyletic group: A. besseyi and A. ritzemabosi cluster together, and they are phylogenetically isolated from A. fragariae, A. subtenuis and other, most fungivorous species. A phylum-wide SSU rDNA framework was used to identify species-specific DNA motifs. For the molecular detection of four plant-parasitic Aphelenchoides species, PCR primers were developed with high, identical annealing temperatures (63°C). Within the molecular framework presented here, these primers can be used for the rapid screening of plant material and soil for the presence of one or multiple foliar nematode species
Original languageEnglish
Pages (from-to)1153-1160
JournalPhytopathology
Volume102
Issue number12
DOIs
Publication statusPublished - 2012

Keywords

  • real-time pcr
  • potato cyst
  • intraspecific variation
  • populations
  • globodera
  • differentiation
  • subtenuis
  • fragariae
  • fragment
  • rdna

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