Size does matter: Cre-mediated somatic deletion efficiency depends on the distance between the target lox-sites

E.R. Coppoolse, M.J. de Vroomen, F. van Gennip, B.J.M. Hersmus, M.J. van Haaren

Research output: Contribution to journalArticleAcademicpeer-review

18 Citations (Scopus)

Abstract

Cre/lox recombination in vivo has become an important tool to induce chromosomal rearrangements like deletions. Using a combination of Ds transposition and Cre/lox recombination in two independent experiments on chromosomes 6 and 7 of tomato, two sets of somatic deletions up to a size of 200 kb were obtained. The efficiency of somatic deletion decreased with increasing deletion size. The largest germinally transmitted deletion had a size of only 55 kb. The results show that Cre-mediated deletion in somatic cells is less efficient when the lox sites are separated over larger distances. A further drop of the deletion efficiency after germinal transmission of the larger deletions can be explained by the probable loss of genes that are of vital importance to gametophyte function. Plasmid rescue of an 8.4 kb circularised deleted DNA showed that the Cre-mediated deletion takes place in tomato as expected. Since the circular Cre-deleted DNA could only be PCR amplified in plant cells where the deletion was not complete, the double-stranded DNA circle is assumed to be instable.
Original languageEnglish
Pages (from-to)687-698
JournalPlant Molecular Biology
Volume58
Issue number5
DOIs
Publication statusPublished - 2005

Keywords

  • transgenic tomato plants
  • chromosome rearrangements
  • gene cloning
  • recombination
  • genome
  • expression
  • dna
  • identification
  • biotechnology
  • transposition

Fingerprint Dive into the research topics of 'Size does matter: Cre-mediated somatic deletion efficiency depends on the distance between the target lox-sites'. Together they form a unique fingerprint.

Cite this