Site-Specific Immobilization of DNA in Glass Microchannels via Photolithography

T. Vong, J. ter Maat, T.A. van Beek, B. van Lagen, M. Giesbers, J.C.M. van Hest, H. Zuilhof

Research output: Contribution to journalArticleAcademicpeer-review

16 Citations (Scopus)


For the first time, it microchannel was photochemically patterned with it functional linker. This simple method was developed for the site-specific attachment of DNA via this linker onto silicon oxide surfaces (e.g., fused silica and borosilicate glass), both onto a flat surface and onto the inside of a fused silica microchannel. Sharp boundaries in the micrometer range between modified and unmodified zones were demonstrated by the attachment Of fluorescently labeled DNA oligomers. Studies of repeated hybridization-deliybridization cycles revealed selective and reversible binding of cDNA strands at the explicit locations. On average, similar to 7 x 10(11) fluorescently labeled DNA molecules were hybridized per square centimeter. The modified surfaces were characterized with X-ray photoelectron spectroscopy, infrared microscopy, static contact angle measurements, confocal laser scanning microscopy, and fluorescence detection (to quantify the attachment of the fluorescently labeled DNA).
Original languageEnglish
Pages (from-to)13952-13958
Issue number24
Publication statusPublished - 2009


  • organic monolayers
  • covalent attachment
  • silicon surfaces
  • micrometer-scale
  • alkyl monolayers
  • electrochemistry
  • lithography
  • microarrays
  • strategies
  • h-si(111)


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