Site-selective and inducible acylation of thrombin using aptamer-catalyst conjugates

Jordi F. Keijzer, Judith Firet, Bauke Albada*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

9 Citations (Scopus)

Abstract

Two acyl-transfer catalysts were conjugated to thrombin-binding DNA aptamers to acylate thrombin. Modification occurred site-selectively on Lys (≫Ser) residues proximal to the respective aptamer-thrombin interface, was selective for thrombin in the presence of other proteins, and the activity of both DNA-catalysts could be controlled by an external trigger.

Original languageEnglish
Pages (from-to)12960-12963
Number of pages4
JournalChemical Communications
Volume57
Issue number96
DOIs
Publication statusPublished - 14 Dec 2021

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