Single-tube linear DNA amplification (LinDA) for robust ChIP-seq

P. Shankaranarayanan; M.A. Mendoza-Parra; M. Walia; L. Wang; N. Li; L.M. Trindade; H. Gronemeyer

doi:10.1038/NMETH.1626

Single-tube linear DNA amplification (LinDA) for robust ChIP-seq

P. Shankaranarayanan, M.A. Mendoza-Parra, M. Walia, L. Wang, N. Li, L.M. Trindade, H. Gronemeyer

Research output: Contribution to journal › Article › Academic › peer-review

108 Citations (Scopus)

Abstract

Genome-wide profiling of transcription factors based on massive parallel sequencing of immunoprecipitated chromatin (ChIP-seq) requires nanogram amounts of DNA. Here we describe a high-fidelity, single-tube linear DNA amplification method (LinDA) for ChIP-seq and reChIP-seq with picogram DNA amounts obtained from a few thousand cells. This amplification technology will facilitate global analyses of transcription-factor binding and chromatin with very small cell populations, such as stem or cancer-initiating cells

Original language	English
Pages (from-to)	565-567
Journal	Nature Methods : techniques for life scientists and chemists
Volume	8
DOIs	https://doi.org/10.1038/NMETH.1626
Publication status	Published - 2011

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10.1038/NMETH.1626

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abstract = "Genome-wide profiling of transcription factors based on massive parallel sequencing of immunoprecipitated chromatin (ChIP-seq) requires nanogram amounts of DNA. Here we describe a high-fidelity, single-tube linear DNA amplification method (LinDA) for ChIP-seq and reChIP-seq with picogram DNA amounts obtained from a few thousand cells. This amplification technology will facilitate global analyses of transcription-factor binding and chromatin with very small cell populations, such as stem or cancer-initiating cells",

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AU - Shankaranarayanan, P.

AU - Mendoza-Parra, M.A.

AU - Walia, M.

AU - Wang, L.

AU - Li, N.

AU - Trindade, L.M.

AU - Gronemeyer, H.

PY - 2011

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N2 - Genome-wide profiling of transcription factors based on massive parallel sequencing of immunoprecipitated chromatin (ChIP-seq) requires nanogram amounts of DNA. Here we describe a high-fidelity, single-tube linear DNA amplification method (LinDA) for ChIP-seq and reChIP-seq with picogram DNA amounts obtained from a few thousand cells. This amplification technology will facilitate global analyses of transcription-factor binding and chromatin with very small cell populations, such as stem or cancer-initiating cells

AB - Genome-wide profiling of transcription factors based on massive parallel sequencing of immunoprecipitated chromatin (ChIP-seq) requires nanogram amounts of DNA. Here we describe a high-fidelity, single-tube linear DNA amplification method (LinDA) for ChIP-seq and reChIP-seq with picogram DNA amounts obtained from a few thousand cells. This amplification technology will facilitate global analyses of transcription-factor binding and chromatin with very small cell populations, such as stem or cancer-initiating cells

U2 - 10.1038/NMETH.1626

DO - 10.1038/NMETH.1626

M3 - Article

SN - 1548-7091

VL - 8

SP - 565

EP - 567

JO - Nature Methods : techniques for life scientists and chemists

JF - Nature Methods : techniques for life scientists and chemists

ER -

Single-tube linear DNA amplification (LinDA) for robust ChIP-seq

Abstract

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