Single-tube linear DNA amplification (LinDA) for robust ChIP-seq

P. Shankaranarayanan, M.A. Mendoza-Parra, M. Walia, L. Wang, N. Li, L.M. Trindade, H. Gronemeyer

Research output: Contribution to journalArticleAcademicpeer-review

107 Citations (Scopus)


Genome-wide profiling of transcription factors based on massive parallel sequencing of immunoprecipitated chromatin (ChIP-seq) requires nanogram amounts of DNA. Here we describe a high-fidelity, single-tube linear DNA amplification method (LinDA) for ChIP-seq and reChIP-seq with picogram DNA amounts obtained from a few thousand cells. This amplification technology will facilitate global analyses of transcription-factor binding and chromatin with very small cell populations, such as stem or cancer-initiating cells
Original languageEnglish
Pages (from-to)565-567
JournalNature Methods : techniques for life scientists and chemists
Publication statusPublished - 2011


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