Single-tube linear DNA amplification for genome-wide studies using a few thousand cells

P. Shankaranarayanan; M.A. Mendoza-Parra; W. van Gool; L.M. Trindade; H. Gronemeyer

doi:10.1038/nprot.2011.447

Single-tube linear DNA amplification for genome-wide studies using a few thousand cells

P. Shankaranarayanan, M.A. Mendoza-Parra, W. van Gool, L.M. Trindade, H. Gronemeyer

Research output: Contribution to journal › Article › Academic › peer-review

28 Citations (Scopus)

Abstract

Linear amplification of DNA (LinDA) by T7 polymerase is a versatile and robust method for generating sufficient amounts of DNA for genome-wide studies with minute amounts of cells. LinDA can be coupled to a great number of global profiling technologies. Indeed, chromatin immunoprecipitation coupled to massive parallel sequencing (ChIP-seq) has been achieved for transcription factors and epigenetic modification of chromatin histones with 1,000 to 5,000 cells. LinDA largely simplifies reChIP-seq experiments to monitor co-binding at chromatin target sites. The single-tube design of LinDA is ideal for handling ultrasmall amounts of DNA (

Original language	English
Pages (from-to)	328-339
Journal	Nature protocols
Volume	7
Issue number	2
DOIs	https://doi.org/10.1038/nprot.2011.447
Publication status	Published - 2012

Keywords

t7 rna-polymerase
chip-seq
displacement amplification
limited numbers
chromatin
methylation
challenges
principles
samples
domain

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10.1038/nprot.2011.447

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abstract = "Linear amplification of DNA (LinDA) by T7 polymerase is a versatile and robust method for generating sufficient amounts of DNA for genome-wide studies with minute amounts of cells. LinDA can be coupled to a great number of global profiling technologies. Indeed, chromatin immunoprecipitation coupled to massive parallel sequencing (ChIP-seq) has been achieved for transcription factors and epigenetic modification of chromatin histones with 1,000 to 5,000 cells. LinDA largely simplifies reChIP-seq experiments to monitor co-binding at chromatin target sites. The single-tube design of LinDA is ideal for handling ultrasmall amounts of DNA (",

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AU - Trindade, L.M.

AU - Gronemeyer, H.

PY - 2012

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KW - chip-seq

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KW - limited numbers

KW - chromatin

KW - methylation

KW - challenges

KW - principles

KW - samples

KW - domain

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JF - Nature protocols

SN - 1754-2189

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Single-tube linear DNA amplification for genome-wide studies using a few thousand cells

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Keywords

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