Single-tube linear DNA amplification for genome-wide studies using a few thousand cells

P. Shankaranarayanan, M.A. Mendoza-Parra, W. van Gool, L.M. Trindade, H. Gronemeyer

Research output: Contribution to journalArticleAcademicpeer-review

26 Citations (Scopus)

Abstract

Linear amplification of DNA (LinDA) by T7 polymerase is a versatile and robust method for generating sufficient amounts of DNA for genome-wide studies with minute amounts of cells. LinDA can be coupled to a great number of global profiling technologies. Indeed, chromatin immunoprecipitation coupled to massive parallel sequencing (ChIP-seq) has been achieved for transcription factors and epigenetic modification of chromatin histones with 1,000 to 5,000 cells. LinDA largely simplifies reChIP-seq experiments to monitor co-binding at chromatin target sites. The single-tube design of LinDA is ideal for handling ultrasmall amounts of DNA (
Original languageEnglish
Pages (from-to)328-339
JournalNature protocols
Volume7
Issue number2
DOIs
Publication statusPublished - 2012

Keywords

  • t7 rna-polymerase
  • chip-seq
  • displacement amplification
  • limited numbers
  • chromatin
  • methylation
  • challenges
  • principles
  • samples
  • domain

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