Abstract
Linear amplification of DNA (LinDA) by T7 polymerase is a versatile and robust method for generating sufficient amounts of DNA for genome-wide studies with minute amounts of cells. LinDA can be coupled to a great number of global profiling technologies. Indeed, chromatin immunoprecipitation coupled to massive parallel sequencing (ChIP-seq) has been achieved for transcription factors and epigenetic modification of chromatin histones with 1,000 to 5,000 cells. LinDA largely simplifies reChIP-seq experiments to monitor co-binding at chromatin target sites. The single-tube design of LinDA is ideal for handling ultrasmall amounts of DNA (
| Original language | English |
|---|---|
| Pages (from-to) | 328-339 |
| Journal | Nature protocols |
| Volume | 7 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - 2012 |
Keywords
- t7 rna-polymerase
- chip-seq
- displacement amplification
- limited numbers
- chromatin
- methylation
- challenges
- principles
- samples
- domain
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Shankaranarayanan, P., Mendoza-Parra, M. A., van Gool, W., Trindade, L. M., & Gronemeyer, H. (2012). Single-tube linear DNA amplification for genome-wide studies using a few thousand cells. Nature protocols, 7(2), 328-339. https://doi.org/10.1038/nprot.2011.447