Single nucleotide polymorphisms in the vitamin D receptor gene (VDR) may have an impact on acute pancreatitis (AP) development

A prospective study in populations of AP patients and alcohol-abuse controls

Anna Cieślińska, Elżbieta Kostyra, Ewa Fiedorowicz, Jadwiga Snarska, Natalia Kordulewska, Krzysztof Kiper, Huub F.J. Savelkoul*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

2 Citations (Scopus)

Abstract

Vitamin D imbalance is suggested to be associated with the development of pancreatitis. Single nucleotide polymorphisms (SNPs), Apa-1, Bsm-1, Fok-1, and Taq-1, in the vitamin D receptor gene (VDR) are known in various diseases, but not yet in pancreatitis. The aim of this study was to explore possible associations of the four SNPs in the VDR receptor gene in a population of acute pancreatitis patients and alcohol-abuse controls, and to investigate the association with acute pancreatitis (AP) susceptibility. The study population (n = 239) included acute pancreatitis patients (n = 129) and an alcohol-abuse control group (n = 110). All patients met the Diagnostic and Statistical Manual of Mental Disorders (DSM IV) criteria for alcohol dependence. DNA was extracted from peripheral leukocytes and analyzed for VDR polymorphisms using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Odd ratios (ORs) and 95% confidence intervals (CIs) were calculated using logistic regression analysis. To date, we have found allele T in Taq-1 (OR = 2.61; 95% CI: 1.68–4.03; p < 0.0001) to be almost three times more frequent in the AP group compared to the alcohol-abuse control patients. Polymorphism Taq-1 occurring in the vitamin D receptor may have an impact on the development of acute pancreatitis due to the lack of the protective role of vitamin D.

Original languageEnglish
Article number1919
JournalInternational Journal of Molecular Sciences
Volume19
Issue number7
DOIs
Publication statusPublished - 1 Jul 2018

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calciferol
Calcitriol Receptors
Vitamins
polymorphism
nucleotides
Nucleotides
Polymorphism
Pancreatitis
genes
Alcoholism
Single Nucleotide Polymorphism
alcohols
Alcohols
Genes
Prospective Studies
Population
Vitamin D
confidence
Diagnostic and Statistical Manual of Mental Disorders
Polymerase chain reaction

Keywords

  • Acute pancreatitis
  • Polymorphism
  • SNP analysis
  • Vitamin D
  • Vitamin D receptor

Cite this

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title = "Single nucleotide polymorphisms in the vitamin D receptor gene (VDR) may have an impact on acute pancreatitis (AP) development: A prospective study in populations of AP patients and alcohol-abuse controls",
abstract = "Vitamin D imbalance is suggested to be associated with the development of pancreatitis. Single nucleotide polymorphisms (SNPs), Apa-1, Bsm-1, Fok-1, and Taq-1, in the vitamin D receptor gene (VDR) are known in various diseases, but not yet in pancreatitis. The aim of this study was to explore possible associations of the four SNPs in the VDR receptor gene in a population of acute pancreatitis patients and alcohol-abuse controls, and to investigate the association with acute pancreatitis (AP) susceptibility. The study population (n = 239) included acute pancreatitis patients (n = 129) and an alcohol-abuse control group (n = 110). All patients met the Diagnostic and Statistical Manual of Mental Disorders (DSM IV) criteria for alcohol dependence. DNA was extracted from peripheral leukocytes and analyzed for VDR polymorphisms using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Odd ratios (ORs) and 95{\%} confidence intervals (CIs) were calculated using logistic regression analysis. To date, we have found allele T in Taq-1 (OR = 2.61; 95{\%} CI: 1.68–4.03; p < 0.0001) to be almost three times more frequent in the AP group compared to the alcohol-abuse control patients. Polymorphism Taq-1 occurring in the vitamin D receptor may have an impact on the development of acute pancreatitis due to the lack of the protective role of vitamin D.",
keywords = "Acute pancreatitis, Polymorphism, SNP analysis, Vitamin D, Vitamin D receptor",
author = "Anna Cieślińska and Elżbieta Kostyra and Ewa Fiedorowicz and Jadwiga Snarska and Natalia Kordulewska and Krzysztof Kiper and Savelkoul, {Huub F.J.}",
year = "2018",
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language = "English",
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Single nucleotide polymorphisms in the vitamin D receptor gene (VDR) may have an impact on acute pancreatitis (AP) development : A prospective study in populations of AP patients and alcohol-abuse controls. / Cieślińska, Anna; Kostyra, Elżbieta; Fiedorowicz, Ewa; Snarska, Jadwiga; Kordulewska, Natalia; Kiper, Krzysztof; Savelkoul, Huub F.J.

In: International Journal of Molecular Sciences, Vol. 19, No. 7, 1919, 01.07.2018.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Single nucleotide polymorphisms in the vitamin D receptor gene (VDR) may have an impact on acute pancreatitis (AP) development

T2 - A prospective study in populations of AP patients and alcohol-abuse controls

AU - Cieślińska, Anna

AU - Kostyra, Elżbieta

AU - Fiedorowicz, Ewa

AU - Snarska, Jadwiga

AU - Kordulewska, Natalia

AU - Kiper, Krzysztof

AU - Savelkoul, Huub F.J.

PY - 2018/7/1

Y1 - 2018/7/1

N2 - Vitamin D imbalance is suggested to be associated with the development of pancreatitis. Single nucleotide polymorphisms (SNPs), Apa-1, Bsm-1, Fok-1, and Taq-1, in the vitamin D receptor gene (VDR) are known in various diseases, but not yet in pancreatitis. The aim of this study was to explore possible associations of the four SNPs in the VDR receptor gene in a population of acute pancreatitis patients and alcohol-abuse controls, and to investigate the association with acute pancreatitis (AP) susceptibility. The study population (n = 239) included acute pancreatitis patients (n = 129) and an alcohol-abuse control group (n = 110). All patients met the Diagnostic and Statistical Manual of Mental Disorders (DSM IV) criteria for alcohol dependence. DNA was extracted from peripheral leukocytes and analyzed for VDR polymorphisms using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Odd ratios (ORs) and 95% confidence intervals (CIs) were calculated using logistic regression analysis. To date, we have found allele T in Taq-1 (OR = 2.61; 95% CI: 1.68–4.03; p < 0.0001) to be almost three times more frequent in the AP group compared to the alcohol-abuse control patients. Polymorphism Taq-1 occurring in the vitamin D receptor may have an impact on the development of acute pancreatitis due to the lack of the protective role of vitamin D.

AB - Vitamin D imbalance is suggested to be associated with the development of pancreatitis. Single nucleotide polymorphisms (SNPs), Apa-1, Bsm-1, Fok-1, and Taq-1, in the vitamin D receptor gene (VDR) are known in various diseases, but not yet in pancreatitis. The aim of this study was to explore possible associations of the four SNPs in the VDR receptor gene in a population of acute pancreatitis patients and alcohol-abuse controls, and to investigate the association with acute pancreatitis (AP) susceptibility. The study population (n = 239) included acute pancreatitis patients (n = 129) and an alcohol-abuse control group (n = 110). All patients met the Diagnostic and Statistical Manual of Mental Disorders (DSM IV) criteria for alcohol dependence. DNA was extracted from peripheral leukocytes and analyzed for VDR polymorphisms using a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Odd ratios (ORs) and 95% confidence intervals (CIs) were calculated using logistic regression analysis. To date, we have found allele T in Taq-1 (OR = 2.61; 95% CI: 1.68–4.03; p < 0.0001) to be almost three times more frequent in the AP group compared to the alcohol-abuse control patients. Polymorphism Taq-1 occurring in the vitamin D receptor may have an impact on the development of acute pancreatitis due to the lack of the protective role of vitamin D.

KW - Acute pancreatitis

KW - Polymorphism

KW - SNP analysis

KW - Vitamin D

KW - Vitamin D receptor

U2 - 10.3390/ijms19071919

DO - 10.3390/ijms19071919

M3 - Article

VL - 19

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1661-6596

IS - 7

M1 - 1919

ER -