Single-laboratory validation of a multiplex flow cytometric immunoassay for the simultaneous detection of coccidiostats in eggs and feed

M.E. Bienenmann-Ploum, U. Vincent, K. Campbell, A.C. Huet, W. Haasnoot, P. Delahaut, A.A.M. Stolker, C.T. Elliott, M.W.F. Nielen

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6 Citations (Scopus)

Abstract

Coccidiostats are authorized in the European Union (EU) to be used as poultry feed additives. Maximum (residue) levels (M(R)Ls) have been set within the EU for consumer and animal protection against unintended carry-over, and monitoring is compulsory. This paper describes the single-laboratory validation of a previously developed multiplex flow cytometric immunoassay (FCIA) as screening method for coccidiostats in eggs and feed and provides and compares different approaches for the calculation of the cut-off levels which are not described in detail within Commission Decision 2002/657/EC. Comparable results were obtained between the statistical (reference) approach and the rapid approaches. With the most rapid approach, the cut-off levels for narasin/salinomycin, lasalocid, diclazuril, nicarbazin (DNC) and monensin in egg, calculated as percentages of inhibition (%B/B0), were 60, 32, 76, 80 and 84, respectively. In feed, the cut-off levels for narasin/salinomycin, lasalocid, nicarbazin (DNC) and monensin were 70, 64, 72 and 78, respectively, and could not be determined for diclazuril. For all analytes, except for diclazuril in feed, the rate of false positives (false non-compliant) in blank samples was lower than 1 %, and the rate of false negatives (false compliant) at the M(R)Ls was below 5 %. Additionally, very good correlations (r ranging from 0.994 to 0.9994) were observed between two different analysers, a sophisticated flow cytometer (FlexMAP 3D®) and a more cost-efficient and transportable planar imaging detector (MAGPIX®), hence demonstrating adequate transferability.
Original languageEnglish
Pages (from-to)9571-9577
JournalAnalytical and Bioanalytical Chemistry
Volume405
Issue number29
DOIs
Publication statusPublished - 2013

Fingerprint

diclazuril
Coccidiostats
Nicarbazin
Immunoassay
Eggs
Lasalocid
Monensin
European Union
Poultry
Ovum
Screening
Animals
Detectors
Imaging techniques
Costs and Cost Analysis
Monitoring

Keywords

  • screening methods

Cite this

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title = "Single-laboratory validation of a multiplex flow cytometric immunoassay for the simultaneous detection of coccidiostats in eggs and feed",
abstract = "Coccidiostats are authorized in the European Union (EU) to be used as poultry feed additives. Maximum (residue) levels (M(R)Ls) have been set within the EU for consumer and animal protection against unintended carry-over, and monitoring is compulsory. This paper describes the single-laboratory validation of a previously developed multiplex flow cytometric immunoassay (FCIA) as screening method for coccidiostats in eggs and feed and provides and compares different approaches for the calculation of the cut-off levels which are not described in detail within Commission Decision 2002/657/EC. Comparable results were obtained between the statistical (reference) approach and the rapid approaches. With the most rapid approach, the cut-off levels for narasin/salinomycin, lasalocid, diclazuril, nicarbazin (DNC) and monensin in egg, calculated as percentages of inhibition ({\%}B/B0), were 60, 32, 76, 80 and 84, respectively. In feed, the cut-off levels for narasin/salinomycin, lasalocid, nicarbazin (DNC) and monensin were 70, 64, 72 and 78, respectively, and could not be determined for diclazuril. For all analytes, except for diclazuril in feed, the rate of false positives (false non-compliant) in blank samples was lower than 1 {\%}, and the rate of false negatives (false compliant) at the M(R)Ls was below 5 {\%}. Additionally, very good correlations (r ranging from 0.994 to 0.9994) were observed between two different analysers, a sophisticated flow cytometer (FlexMAP 3D{\circledR}) and a more cost-efficient and transportable planar imaging detector (MAGPIX{\circledR}), hence demonstrating adequate transferability.",
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author = "M.E. Bienenmann-Ploum and U. Vincent and K. Campbell and A.C. Huet and W. Haasnoot and P. Delahaut and A.A.M. Stolker and C.T. Elliott and M.W.F. Nielen",
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Single-laboratory validation of a multiplex flow cytometric immunoassay for the simultaneous detection of coccidiostats in eggs and feed. / Bienenmann-Ploum, M.E.; Vincent, U.; Campbell, K.; Huet, A.C.; Haasnoot, W.; Delahaut, P.; Stolker, A.A.M.; Elliott, C.T.; Nielen, M.W.F.

In: Analytical and Bioanalytical Chemistry, Vol. 405, No. 29, 2013, p. 9571-9577.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Single-laboratory validation of a multiplex flow cytometric immunoassay for the simultaneous detection of coccidiostats in eggs and feed

AU - Bienenmann-Ploum, M.E.

AU - Vincent, U.

AU - Campbell, K.

AU - Huet, A.C.

AU - Haasnoot, W.

AU - Delahaut, P.

AU - Stolker, A.A.M.

AU - Elliott, C.T.

AU - Nielen, M.W.F.

PY - 2013

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N2 - Coccidiostats are authorized in the European Union (EU) to be used as poultry feed additives. Maximum (residue) levels (M(R)Ls) have been set within the EU for consumer and animal protection against unintended carry-over, and monitoring is compulsory. This paper describes the single-laboratory validation of a previously developed multiplex flow cytometric immunoassay (FCIA) as screening method for coccidiostats in eggs and feed and provides and compares different approaches for the calculation of the cut-off levels which are not described in detail within Commission Decision 2002/657/EC. Comparable results were obtained between the statistical (reference) approach and the rapid approaches. With the most rapid approach, the cut-off levels for narasin/salinomycin, lasalocid, diclazuril, nicarbazin (DNC) and monensin in egg, calculated as percentages of inhibition (%B/B0), were 60, 32, 76, 80 and 84, respectively. In feed, the cut-off levels for narasin/salinomycin, lasalocid, nicarbazin (DNC) and monensin were 70, 64, 72 and 78, respectively, and could not be determined for diclazuril. For all analytes, except for diclazuril in feed, the rate of false positives (false non-compliant) in blank samples was lower than 1 %, and the rate of false negatives (false compliant) at the M(R)Ls was below 5 %. Additionally, very good correlations (r ranging from 0.994 to 0.9994) were observed between two different analysers, a sophisticated flow cytometer (FlexMAP 3D®) and a more cost-efficient and transportable planar imaging detector (MAGPIX®), hence demonstrating adequate transferability.

AB - Coccidiostats are authorized in the European Union (EU) to be used as poultry feed additives. Maximum (residue) levels (M(R)Ls) have been set within the EU for consumer and animal protection against unintended carry-over, and monitoring is compulsory. This paper describes the single-laboratory validation of a previously developed multiplex flow cytometric immunoassay (FCIA) as screening method for coccidiostats in eggs and feed and provides and compares different approaches for the calculation of the cut-off levels which are not described in detail within Commission Decision 2002/657/EC. Comparable results were obtained between the statistical (reference) approach and the rapid approaches. With the most rapid approach, the cut-off levels for narasin/salinomycin, lasalocid, diclazuril, nicarbazin (DNC) and monensin in egg, calculated as percentages of inhibition (%B/B0), were 60, 32, 76, 80 and 84, respectively. In feed, the cut-off levels for narasin/salinomycin, lasalocid, nicarbazin (DNC) and monensin were 70, 64, 72 and 78, respectively, and could not be determined for diclazuril. For all analytes, except for diclazuril in feed, the rate of false positives (false non-compliant) in blank samples was lower than 1 %, and the rate of false negatives (false compliant) at the M(R)Ls was below 5 %. Additionally, very good correlations (r ranging from 0.994 to 0.9994) were observed between two different analysers, a sophisticated flow cytometer (FlexMAP 3D®) and a more cost-efficient and transportable planar imaging detector (MAGPIX®), hence demonstrating adequate transferability.

KW - screening methods

U2 - 10.1007/s00216-013-7362-7

DO - 10.1007/s00216-013-7362-7

M3 - Article

VL - 405

SP - 9571

EP - 9577

JO - Analytical and Bioanalytical Chemistry

JF - Analytical and Bioanalytical Chemistry

SN - 1618-2642

IS - 29

ER -