Simultaneous quantification of differently glycosylated, acetylated and 2,3-dihydro-2,5-dihydroxy-6-methyl-4h-pyran-4-one-conjugated soyasaponins using reversed-phase high-performance liquid chromatography with evaporative light scattering detection

K. DeCroos, J.P. Vincken, L. Heng, R. Bakker, H. Gruppen, W. Verstraete

Research output: Contribution to journalArticleAcademicpeer-review

36 Citations (Scopus)

Abstract

A novel method utilizing high-performance liquid chromatography (HPLC) with evaporative light scattering detection (ELSD) and electrospray ionisation mass spectrometry (ESI-MS) was developed for the analysis of soyasaponins, a divers group of triterpenic compounds with one or two sugar side chains, occurring in soy. Group A soyasaponins in different degrees of acetylation, as well as group B soyasaponins in both their 2,3-dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one (DDMP)-conjugated and non-conjugated forms could be separated and quantified using authentic soyasaponin standards, in one single run. The method was tested by the determination of the soyasaponin content and composition of eight soygerm samples of different origin. Differences in the composition and the degree of acetylation of the group A soyasaponins were observed among these samples. The group B soyasaponins showed much less variability and they were mainly present in their DDMP-conjugated form.
Original languageEnglish
Pages (from-to)185-193
JournalJournal of Chromatography. A, Including electrophoresis and other separation methods
Volume1072
Issue number2
DOIs
Publication statusPublished - 2005

Keywords

  • group-a saponin
  • soybean seed
  • soyasapogenol-a
  • glycine-max
  • in-vitro
  • sapogenol
  • food
  • soy
  • varieties
  • ginseng

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