Simultaneous Quantification and Differentiation of Streptococcus suis Serotypes 2 and 9 by Quantitative Real-time PCR, Evaluated in Tonsillar and Nasal Samples of Pigs

Niels Dekker, Ineke Daemen, K.M. Verstappen, A. de Greeff, H.E. Smith, Birgitta Duim

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Abstract

Abstract Invasive Streptococcus suis (S. suis) infections in pigs are often associated with serotypes 2 and 9. Mucosal sites of healthy pigs can be colonized with these serotypes, often multiple serotypes per pig. To unravel the contribution of these serotypes in pathogenesis and epidemiology, simultaneous quantification of serotypes is needed. A quantitative real-time PCR (qPCR) targeting cps2J (serotypes 2 and 1/2) and cps9H (serotype 9) was evaluated with nasal and tonsillar samples from S. suis exposed pigs. qPCR specifically detected serotypes in all pig samples. The serotypes loads in pig samples estimated by qPCR showed, except for serotype 9 in tonsillar samples (correlation coefficient = 0.25), moderate to strong correlation with loads detected by culture (correlation coefficient > 0.65), and also in pigs exposed to both serotypes (correlation coefficient > 0.75). This qPCR is suitable for simultaneous differentiation and quantification of important S. suis serotypes
Original languageEnglish
Article number46
JournalPathogens
Volume5
Issue number3
DOIs
Publication statusPublished - 2016

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Streptococcus suis
Nose
Real-Time Polymerase Chain Reaction
Swine
Serogroup
Epidemiology

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@article{af002d6e743546f2953bf67c48f6b7ee,
title = "Simultaneous Quantification and Differentiation of Streptococcus suis Serotypes 2 and 9 by Quantitative Real-time PCR, Evaluated in Tonsillar and Nasal Samples of Pigs",
abstract = "Abstract Invasive Streptococcus suis (S. suis) infections in pigs are often associated with serotypes 2 and 9. Mucosal sites of healthy pigs can be colonized with these serotypes, often multiple serotypes per pig. To unravel the contribution of these serotypes in pathogenesis and epidemiology, simultaneous quantification of serotypes is needed. A quantitative real-time PCR (qPCR) targeting cps2J (serotypes 2 and 1/2) and cps9H (serotype 9) was evaluated with nasal and tonsillar samples from S. suis exposed pigs. qPCR specifically detected serotypes in all pig samples. The serotypes loads in pig samples estimated by qPCR showed, except for serotype 9 in tonsillar samples (correlation coefficient = 0.25), moderate to strong correlation with loads detected by culture (correlation coefficient > 0.65), and also in pigs exposed to both serotypes (correlation coefficient > 0.75). This qPCR is suitable for simultaneous differentiation and quantification of important S. suis serotypes",
author = "Niels Dekker and Ineke Daemen and K.M. Verstappen and {de Greeff}, A. and H.E. Smith and Birgitta Duim",
year = "2016",
doi = "10.3390/pathogens5030046",
language = "English",
volume = "5",
journal = "Pathogens",
issn = "2076-0817",
publisher = "MDPI",
number = "3",

}

Simultaneous Quantification and Differentiation of Streptococcus suis Serotypes 2 and 9 by Quantitative Real-time PCR, Evaluated in Tonsillar and Nasal Samples of Pigs. / Dekker, Niels; Daemen, Ineke; Verstappen, K.M.; de Greeff, A.; Smith, H.E.; Duim, Birgitta.

In: Pathogens, Vol. 5, No. 3, 46, 2016.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Simultaneous Quantification and Differentiation of Streptococcus suis Serotypes 2 and 9 by Quantitative Real-time PCR, Evaluated in Tonsillar and Nasal Samples of Pigs

AU - Dekker, Niels

AU - Daemen, Ineke

AU - Verstappen, K.M.

AU - de Greeff, A.

AU - Smith, H.E.

AU - Duim, Birgitta

PY - 2016

Y1 - 2016

N2 - Abstract Invasive Streptococcus suis (S. suis) infections in pigs are often associated with serotypes 2 and 9. Mucosal sites of healthy pigs can be colonized with these serotypes, often multiple serotypes per pig. To unravel the contribution of these serotypes in pathogenesis and epidemiology, simultaneous quantification of serotypes is needed. A quantitative real-time PCR (qPCR) targeting cps2J (serotypes 2 and 1/2) and cps9H (serotype 9) was evaluated with nasal and tonsillar samples from S. suis exposed pigs. qPCR specifically detected serotypes in all pig samples. The serotypes loads in pig samples estimated by qPCR showed, except for serotype 9 in tonsillar samples (correlation coefficient = 0.25), moderate to strong correlation with loads detected by culture (correlation coefficient > 0.65), and also in pigs exposed to both serotypes (correlation coefficient > 0.75). This qPCR is suitable for simultaneous differentiation and quantification of important S. suis serotypes

AB - Abstract Invasive Streptococcus suis (S. suis) infections in pigs are often associated with serotypes 2 and 9. Mucosal sites of healthy pigs can be colonized with these serotypes, often multiple serotypes per pig. To unravel the contribution of these serotypes in pathogenesis and epidemiology, simultaneous quantification of serotypes is needed. A quantitative real-time PCR (qPCR) targeting cps2J (serotypes 2 and 1/2) and cps9H (serotype 9) was evaluated with nasal and tonsillar samples from S. suis exposed pigs. qPCR specifically detected serotypes in all pig samples. The serotypes loads in pig samples estimated by qPCR showed, except for serotype 9 in tonsillar samples (correlation coefficient = 0.25), moderate to strong correlation with loads detected by culture (correlation coefficient > 0.65), and also in pigs exposed to both serotypes (correlation coefficient > 0.75). This qPCR is suitable for simultaneous differentiation and quantification of important S. suis serotypes

U2 - 10.3390/pathogens5030046

DO - 10.3390/pathogens5030046

M3 - Article

VL - 5

JO - Pathogens

JF - Pathogens

SN - 2076-0817

IS - 3

M1 - 46

ER -