Simultaneous determination of aflatoxin B1, fumonisin B1 and deoxynivalenol in beer samples with a label-free monolithically integrated optoelectronic biosensor

Varvara Pagkali, Panagiota S. Petrou*, Eleni Makarona, Jeroen Peters, Willem Haasnoot, Gerhard Jobst, Isabella Moser, Katarzyna Gajos, Andrzej Budkowski, Anastasios Economou, Konstantinos Misiakos, Ioannis Raptis, Sotirios E. Kakabakos

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

8 Citations (Scopus)

Abstract

A label-free optical biosensor for the fast simultaneous determination of three mycotoxins, aflatoxin B1 (AFB1), fumonisin B1 (FB1) and deoxynivalenol (DON), in beer samples is presented. The biosensor is based on an array of ten Mach-Zehnder interferometers (MZIs) monolithically integrated along with their respective broad-band silicon light sources onto a single chip. Multi-analyte determination is accomplished by functionalizing the sensing arms of individual MZIs with mycotoxin-protein conjugates. Assay is performed by pumping over the chip mixtures of calibrators or samples with a mixture of specific monoclonal antibodies, followed by reaction with a secondary anti-mouse IgG antibody. Reactions are monitored in real-time by continuously recording the MZI output spectra, which are then subjected to Discrete Fourier Transform to convert spectrum shifts to phase shifts. The detection limits achieved for AFB1, FB1 and DON were 0.8, 5.6 and 24 ng/ml, respectively, while the assay duration was 12 min. Recovery values ranging from 85 to 115% were determined in beer samples spiked with known concentrations of the three mycotoxins. In addition, beers of different types and origin were analysed with the biosensor developed and the results were compared with those provided by established laboratory methods, further supporting the accuracy of the proposed device.

Original languageEnglish
Pages (from-to)445-453
JournalJournal of Hazardous Materials
Volume359
DOIs
Publication statusPublished - 5 Oct 2018

Fingerprint

Aflatoxins
Integrated optoelectronics
Beer
Aflatoxin B1
Mach-Zehnder interferometers
Mycotoxins
Biosensing Techniques
interferometer
Biosensors
Labels
antibody
Assays
assay
laboratory method
Monoclonal antibodies
Silicon
Fourier Analysis
Phase shift
Antibodies
Discrete Fourier transforms

Keywords

  • Aflatoxin B
  • Beer
  • Deoxynivalenol
  • Fumonisin B
  • Monolithically integrated Mach-Zehnder interferometers

Cite this

Pagkali, Varvara ; Petrou, Panagiota S. ; Makarona, Eleni ; Peters, Jeroen ; Haasnoot, Willem ; Jobst, Gerhard ; Moser, Isabella ; Gajos, Katarzyna ; Budkowski, Andrzej ; Economou, Anastasios ; Misiakos, Konstantinos ; Raptis, Ioannis ; Kakabakos, Sotirios E. / Simultaneous determination of aflatoxin B1, fumonisin B1 and deoxynivalenol in beer samples with a label-free monolithically integrated optoelectronic biosensor. In: Journal of Hazardous Materials. 2018 ; Vol. 359. pp. 445-453.
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title = "Simultaneous determination of aflatoxin B1, fumonisin B1 and deoxynivalenol in beer samples with a label-free monolithically integrated optoelectronic biosensor",
abstract = "A label-free optical biosensor for the fast simultaneous determination of three mycotoxins, aflatoxin B1 (AFB1), fumonisin B1 (FB1) and deoxynivalenol (DON), in beer samples is presented. The biosensor is based on an array of ten Mach-Zehnder interferometers (MZIs) monolithically integrated along with their respective broad-band silicon light sources onto a single chip. Multi-analyte determination is accomplished by functionalizing the sensing arms of individual MZIs with mycotoxin-protein conjugates. Assay is performed by pumping over the chip mixtures of calibrators or samples with a mixture of specific monoclonal antibodies, followed by reaction with a secondary anti-mouse IgG antibody. Reactions are monitored in real-time by continuously recording the MZI output spectra, which are then subjected to Discrete Fourier Transform to convert spectrum shifts to phase shifts. The detection limits achieved for AFB1, FB1 and DON were 0.8, 5.6 and 24 ng/ml, respectively, while the assay duration was 12 min. Recovery values ranging from 85 to 115{\%} were determined in beer samples spiked with known concentrations of the three mycotoxins. In addition, beers of different types and origin were analysed with the biosensor developed and the results were compared with those provided by established laboratory methods, further supporting the accuracy of the proposed device.",
keywords = "Aflatoxin B, Beer, Deoxynivalenol, Fumonisin B, Monolithically integrated Mach-Zehnder interferometers",
author = "Varvara Pagkali and Petrou, {Panagiota S.} and Eleni Makarona and Jeroen Peters and Willem Haasnoot and Gerhard Jobst and Isabella Moser and Katarzyna Gajos and Andrzej Budkowski and Anastasios Economou and Konstantinos Misiakos and Ioannis Raptis and Kakabakos, {Sotirios E.}",
year = "2018",
month = "10",
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Pagkali, V, Petrou, PS, Makarona, E, Peters, J, Haasnoot, W, Jobst, G, Moser, I, Gajos, K, Budkowski, A, Economou, A, Misiakos, K, Raptis, I & Kakabakos, SE 2018, 'Simultaneous determination of aflatoxin B1, fumonisin B1 and deoxynivalenol in beer samples with a label-free monolithically integrated optoelectronic biosensor', Journal of Hazardous Materials, vol. 359, pp. 445-453. https://doi.org/10.1016/j.jhazmat.2018.07.080

Simultaneous determination of aflatoxin B1, fumonisin B1 and deoxynivalenol in beer samples with a label-free monolithically integrated optoelectronic biosensor. / Pagkali, Varvara; Petrou, Panagiota S.; Makarona, Eleni; Peters, Jeroen; Haasnoot, Willem; Jobst, Gerhard; Moser, Isabella; Gajos, Katarzyna; Budkowski, Andrzej; Economou, Anastasios; Misiakos, Konstantinos; Raptis, Ioannis; Kakabakos, Sotirios E.

In: Journal of Hazardous Materials, Vol. 359, 05.10.2018, p. 445-453.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Simultaneous determination of aflatoxin B1, fumonisin B1 and deoxynivalenol in beer samples with a label-free monolithically integrated optoelectronic biosensor

AU - Pagkali, Varvara

AU - Petrou, Panagiota S.

AU - Makarona, Eleni

AU - Peters, Jeroen

AU - Haasnoot, Willem

AU - Jobst, Gerhard

AU - Moser, Isabella

AU - Gajos, Katarzyna

AU - Budkowski, Andrzej

AU - Economou, Anastasios

AU - Misiakos, Konstantinos

AU - Raptis, Ioannis

AU - Kakabakos, Sotirios E.

PY - 2018/10/5

Y1 - 2018/10/5

N2 - A label-free optical biosensor for the fast simultaneous determination of three mycotoxins, aflatoxin B1 (AFB1), fumonisin B1 (FB1) and deoxynivalenol (DON), in beer samples is presented. The biosensor is based on an array of ten Mach-Zehnder interferometers (MZIs) monolithically integrated along with their respective broad-band silicon light sources onto a single chip. Multi-analyte determination is accomplished by functionalizing the sensing arms of individual MZIs with mycotoxin-protein conjugates. Assay is performed by pumping over the chip mixtures of calibrators or samples with a mixture of specific monoclonal antibodies, followed by reaction with a secondary anti-mouse IgG antibody. Reactions are monitored in real-time by continuously recording the MZI output spectra, which are then subjected to Discrete Fourier Transform to convert spectrum shifts to phase shifts. The detection limits achieved for AFB1, FB1 and DON were 0.8, 5.6 and 24 ng/ml, respectively, while the assay duration was 12 min. Recovery values ranging from 85 to 115% were determined in beer samples spiked with known concentrations of the three mycotoxins. In addition, beers of different types and origin were analysed with the biosensor developed and the results were compared with those provided by established laboratory methods, further supporting the accuracy of the proposed device.

AB - A label-free optical biosensor for the fast simultaneous determination of three mycotoxins, aflatoxin B1 (AFB1), fumonisin B1 (FB1) and deoxynivalenol (DON), in beer samples is presented. The biosensor is based on an array of ten Mach-Zehnder interferometers (MZIs) monolithically integrated along with their respective broad-band silicon light sources onto a single chip. Multi-analyte determination is accomplished by functionalizing the sensing arms of individual MZIs with mycotoxin-protein conjugates. Assay is performed by pumping over the chip mixtures of calibrators or samples with a mixture of specific monoclonal antibodies, followed by reaction with a secondary anti-mouse IgG antibody. Reactions are monitored in real-time by continuously recording the MZI output spectra, which are then subjected to Discrete Fourier Transform to convert spectrum shifts to phase shifts. The detection limits achieved for AFB1, FB1 and DON were 0.8, 5.6 and 24 ng/ml, respectively, while the assay duration was 12 min. Recovery values ranging from 85 to 115% were determined in beer samples spiked with known concentrations of the three mycotoxins. In addition, beers of different types and origin were analysed with the biosensor developed and the results were compared with those provided by established laboratory methods, further supporting the accuracy of the proposed device.

KW - Aflatoxin B

KW - Beer

KW - Deoxynivalenol

KW - Fumonisin B

KW - Monolithically integrated Mach-Zehnder interferometers

U2 - 10.1016/j.jhazmat.2018.07.080

DO - 10.1016/j.jhazmat.2018.07.080

M3 - Article

VL - 359

SP - 445

EP - 453

JO - Journal of Hazardous Materials

JF - Journal of Hazardous Materials

SN - 0304-3894

ER -