Simultaneous detection of different Rhizobium strains marked with either the Escherichia coli gusA gene or the Pyrococcus furiosus celB gene.

A. Sessitsch, K.J. Wilson, A.D.L. Akkermans, W.M. de Vos

Research output: Contribution to journalArticleAcademicpeer-review

23 Citations (Scopus)

Abstract

A new marker system for gram-negative bacteria was developed on the basis of the celB gene from the hyperthermophilic archaeon Pyrococcus furiosus, which encodes a thermostable beta-glucosidase with a high level of beta-galactosidase activity. The celB gene is highly suitable as a marker for studying plant-bacterium interaction because endogenous background beta-glucosidase and beta-galactosidase enzyme activity can readily be inactivated by heat and because inexpensive substrates for detection are commercially available. Two celB-expressing transposons were constructed for use in ecological studies of a variety of gram-negative bacteria. The combined use of the gusA marker gene and celB allowed the simultaneous detection of several Rhizobium strains on a plant, and multiple-strain occupancy of individual modules also could be easily detected.
Original languageEnglish
Pages (from-to)4191-4194
JournalApplied and Environmental Microbiology
Volume62
Issue number11
Publication statusPublished - 1996

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