Simulating distal gut mucosal and luminal communities using packed-column biofilm reactors and an in vitro chemostat model

J.A. McDonald, S. Fuentes, K. Schroeter, G.A.M. Heikamp-de Jong, C.M. Khursigara, W.M. de Vos, E. Allen-Vercoe

Research output: Contribution to journalArticleAcademicpeer-review

33 Citations (Scopus)

Abstract

In vivo studies of human mucosal gut microbiota are often limited to end-point analyses and confounded by bowel cleansing procedures. Therefore, we used biofilm reactors to incorporate a simulated mucosal environment into an in vitro gut chemostat model. Communities developed were complex, reproducible, distinct, and representative of in vivo communities.
Original languageEnglish
Pages (from-to)36-44
JournalJournal of Microbiological Methods
Volume108
DOIs
Publication statusPublished - 2015

Keywords

  • human intestinal microbiota
  • motile bacteria
  • clindamycin
  • mucin
  • diversity
  • colonization
  • resistance
  • stability
  • system
  • akkermansia

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