Silencing of six susceptibility genes results in potato late blight resistance

Kaile Sun, Anne-Marie A. Wolters, Jack H. Vossen, Maarten E. Rouwet, Annelies E.H.M. Loonen, Evert Jacobsen, Richard G.F. Visser, Yuling Bai*

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

14 Citations (Scopus)

Abstract

Phytophthora infestans, the causal agent of late blight, is a major threat to commercial potato production worldwide. Significant costs are required for crop protection to secure yield. Many dominant genes for resistance (R-genes) to potato late blight have been identified, and some of these R-genes have been applied in potato breeding. However, the P. infestans population rapidly accumulates new virulent strains that render R-genes ineffective. Here we introduce a new class of resistance which is based on the loss-of-function of a susceptibility gene (S-gene) encoding a product exploited by pathogens during infection and colonization. Impaired S-genes primarily result in recessive resistance traits in contrast to recognition-based resistance that is governed by dominant R-genes. In Arabidopsis thaliana, many S-genes have been detected in screens of mutant populations. In the present study, we selected 11 A. thalianaS-genes and silenced orthologous genes in the potato cultivar Desiree, which is highly susceptible to late blight. The silencing of five genes resulted in complete resistance to the P. infestans isolate Pic99189, and the silencing of a sixth S-gene resulted in reduced susceptibility. The application of S-genes to potato breeding for resistance to late blight is further discussed.

Original languageEnglish
Pages (from-to)731-742
JournalTransgenic Research
Volume25
Issue number5
DOIs
Publication statusPublished - 2016

Fingerprint

Phytophthora infestans
Solanum tuberosum
vpr Genes
Genes
genes
Dominant Genes
potatoes
Breeding
Gene Silencing
Arabidopsis
Population
breeding
dominant genes
gene silencing
Costs and Cost Analysis
plant protection
Infection
Arabidopsis thaliana
mutants

Keywords

  • Late blight
  • Potato
  • Resistance
  • RNAi
  • Susceptibility gene

Cite this

@article{487ae9e4854249a8a810f18d12e979f2,
title = "Silencing of six susceptibility genes results in potato late blight resistance",
abstract = "Phytophthora infestans, the causal agent of late blight, is a major threat to commercial potato production worldwide. Significant costs are required for crop protection to secure yield. Many dominant genes for resistance (R-genes) to potato late blight have been identified, and some of these R-genes have been applied in potato breeding. However, the P. infestans population rapidly accumulates new virulent strains that render R-genes ineffective. Here we introduce a new class of resistance which is based on the loss-of-function of a susceptibility gene (S-gene) encoding a product exploited by pathogens during infection and colonization. Impaired S-genes primarily result in recessive resistance traits in contrast to recognition-based resistance that is governed by dominant R-genes. In Arabidopsis thaliana, many S-genes have been detected in screens of mutant populations. In the present study, we selected 11 A. thalianaS-genes and silenced orthologous genes in the potato cultivar Desiree, which is highly susceptible to late blight. The silencing of five genes resulted in complete resistance to the P. infestans isolate Pic99189, and the silencing of a sixth S-gene resulted in reduced susceptibility. The application of S-genes to potato breeding for resistance to late blight is further discussed.",
keywords = "Late blight, Potato, Resistance, RNAi, Susceptibility gene",
author = "Kaile Sun and Wolters, {Anne-Marie A.} and Vossen, {Jack H.} and Rouwet, {Maarten E.} and Loonen, {Annelies E.H.M.} and Evert Jacobsen and Visser, {Richard G.F.} and Yuling Bai",
year = "2016",
doi = "10.1007/s11248-016-9964-2",
language = "English",
volume = "25",
pages = "731--742",
journal = "Transgenic Research",
issn = "0962-8819",
publisher = "Springer Verlag",
number = "5",

}

Silencing of six susceptibility genes results in potato late blight resistance. / Sun, Kaile; Wolters, Anne-Marie A.; Vossen, Jack H.; Rouwet, Maarten E.; Loonen, Annelies E.H.M.; Jacobsen, Evert; Visser, Richard G.F.; Bai, Yuling.

In: Transgenic Research, Vol. 25, No. 5, 2016, p. 731-742.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Silencing of six susceptibility genes results in potato late blight resistance

AU - Sun, Kaile

AU - Wolters, Anne-Marie A.

AU - Vossen, Jack H.

AU - Rouwet, Maarten E.

AU - Loonen, Annelies E.H.M.

AU - Jacobsen, Evert

AU - Visser, Richard G.F.

AU - Bai, Yuling

PY - 2016

Y1 - 2016

N2 - Phytophthora infestans, the causal agent of late blight, is a major threat to commercial potato production worldwide. Significant costs are required for crop protection to secure yield. Many dominant genes for resistance (R-genes) to potato late blight have been identified, and some of these R-genes have been applied in potato breeding. However, the P. infestans population rapidly accumulates new virulent strains that render R-genes ineffective. Here we introduce a new class of resistance which is based on the loss-of-function of a susceptibility gene (S-gene) encoding a product exploited by pathogens during infection and colonization. Impaired S-genes primarily result in recessive resistance traits in contrast to recognition-based resistance that is governed by dominant R-genes. In Arabidopsis thaliana, many S-genes have been detected in screens of mutant populations. In the present study, we selected 11 A. thalianaS-genes and silenced orthologous genes in the potato cultivar Desiree, which is highly susceptible to late blight. The silencing of five genes resulted in complete resistance to the P. infestans isolate Pic99189, and the silencing of a sixth S-gene resulted in reduced susceptibility. The application of S-genes to potato breeding for resistance to late blight is further discussed.

AB - Phytophthora infestans, the causal agent of late blight, is a major threat to commercial potato production worldwide. Significant costs are required for crop protection to secure yield. Many dominant genes for resistance (R-genes) to potato late blight have been identified, and some of these R-genes have been applied in potato breeding. However, the P. infestans population rapidly accumulates new virulent strains that render R-genes ineffective. Here we introduce a new class of resistance which is based on the loss-of-function of a susceptibility gene (S-gene) encoding a product exploited by pathogens during infection and colonization. Impaired S-genes primarily result in recessive resistance traits in contrast to recognition-based resistance that is governed by dominant R-genes. In Arabidopsis thaliana, many S-genes have been detected in screens of mutant populations. In the present study, we selected 11 A. thalianaS-genes and silenced orthologous genes in the potato cultivar Desiree, which is highly susceptible to late blight. The silencing of five genes resulted in complete resistance to the P. infestans isolate Pic99189, and the silencing of a sixth S-gene resulted in reduced susceptibility. The application of S-genes to potato breeding for resistance to late blight is further discussed.

KW - Late blight

KW - Potato

KW - Resistance

KW - RNAi

KW - Susceptibility gene

U2 - 10.1007/s11248-016-9964-2

DO - 10.1007/s11248-016-9964-2

M3 - Article

VL - 25

SP - 731

EP - 742

JO - Transgenic Research

JF - Transgenic Research

SN - 0962-8819

IS - 5

ER -