Signature genes as a phylogenomic tool

Bas E. Dutilh, Berend Snel, Thijs J.G. Ettema, Martijn A. Huynen

Research output: Contribution to journalArticleAcademicpeer-review

46 Citations (Scopus)


Gene content has been shown to contain a strong phylogenetic signal, yet its usage for phylogenetic questions is hampered by horizontal gene transfer and parallel gene loss and until now required completely sequenced genomes. Here, we introduce an approach that allows the phylogenetic signal in gene content to be applied to any set of sequences, using signature genes for phylogenetic classification. The hundreds of publicly available genomes allow us to identify signature genes at various taxonomic depths, and we show how the presence of signature genes in an unspecified sample can be used to characterize its taxonomic composition. We identify 8,362 signature genes specific for 112 prokaryotic taxa. We show that these signature genes can be used to address phylogenetic questions on the basis of gene content in cases where classic gene content or sequence analyses provide an ambiguous answer, such as for Nanoarchaeum equitans, and even in cases where complete genomes are not available, such as for metagenomics data. Cross-validation experiments leaving out up to 30% of the species show that ∼92% of the signature genes correctly place the species in a related clade. Analyses of metagenomics data sets with the signature gene approach are in good agreement with the previously reported species distributions based on phylogenetic analysis of marker genes. Summarizing, signature genes can complement traditional sequence-based methods in addressing taxonomic questions.

Original languageEnglish
Pages (from-to)1659-1667
Number of pages9
JournalMolecular Biology and Evolution
Issue number8
Publication statusPublished - 1 Aug 2008
Externally publishedYes


  • Gene content
  • Metagenomics
  • Phylogenomics
  • Signature genes
  • Slow-fast


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