Signal transduction pathway(s) in guard cells after prolonged exposure to low vapour pressure deficit

S. Ali Niaei Fard

Research output: Thesisinternal PhD, WUAcademic

Abstract

Keywords: Abscisic acid, Arabidopsis thaliana, calcium, CYP707As, desiccation, environmental factors, guard cells’ signalling pathway, hydrogen peroxide, natural variation, nitric oxide, photosystem II efficiency, RD29A, relative water content, secondary messengers, stomata, vapour pressure deficit, Vicia faba

In short-term, guard cells close stomata in response to an increase in vapour pressure deficit (VPD) and they open the stomata after exposure to low VPDs. However, in long-term responses to low VPD, adaptation processes occur which make stomata less sensitive to stimuli which usually induce stomatal closure (stomatal malfunctioning). Cellular mechanism(s) leading to occurrence of stomatal malfunctioning is (are) still unknown. The aim of this project was to elucidate the processes that are involved in the malfunctioning of stomata after long-term exposure to low VPD. To elucidate whether the problem of stomatal malfunctioning is due to alterations in stomatal morphology and leaf anatomy or in the ABA signalling pathway, fava bean plants were grown at low or moderate VPDs and some plants that developed their leaves at moderate VPD were then transferred for four days to low VPD. Leaf anatomical and stomatal morphological alterations due to low VPD were not the main reason of stomatal malfunctioning in response to ABA and desiccation. Within one day exposure to low VPD, the level of foliar ABA decreased to the same level as in low VPD-grown plants, while the level of ABA-glucose ester was not affected. Spraying ABA during a 4-day exposure to low VPD maintained closing ability of the stomata after 4-day low VPD-exposure. Therefore, alteration in the signalling pathways due to low foliar ABA level was recognized as the main reason for stomatal malfunctioning after long-term low VPD-exposure. Coincidence in changes of Ca2+, ABA receptors, and positive and negative regulators of ABA signalling are proposed as early steps for stomatal malfunctioning induced by low VPD-exposure. Transcriptional activators, transcriptional repressors as well as E3 ligases are proposed for long-term adaptation of cellular processes which consequently cause decreased stomatal response to closing stimuli afterwards. In order to find the molecular mechanism(s) of stomatal malfunctioning, possible variation in stomatal response to closing stimuli was studied among Arabidopsis thaliana accessions after a 4-day low VPD-exposure. Accessions could be grouped to very sensitive, moderately sensitive and less sensitive to closing stimuli using principle component analysis. A positive correlation was found between foliar ABA level (before desiccation) and stomatal closure response to ABA (but not to desiccation) after exposure to different VPDs. Stomatal response to desiccation was positively correlated with the foliar ABA level after desiccation. In order to elucidate the molecular network underlying stomatal malfunctioning in response to ABA due to long-term low VPD-exposure, two groups of Arabidopsis accessions were used as accessions that maintained responsiveness to ABA after low VPD-exposure and accessions with low VPD induced non-ABA-responsive stomata. The foliar ABA content in all accessions correlated with the stomatal response to ABA: only when the ABA level was above a threshold value, stomata responded to ABA. After low VPD-exposure, mainly due to catabolism of ABA, the foliar ABA content decreased. This decrease in ABA level resulted in down regulation of RD29A, which caused decreased stomatal responsiveness to ABA.

 

Original languageEnglish
QualificationDoctor of Philosophy
Awarding Institution
  • Wageningen University
Supervisors/Advisors
  • Woltering, Ernst, Promotor
  • van Meeteren, Uulke, Co-promotor
Award date3 Sep 2014
Place of PublicationWageningen
Publisher
Print ISBNs9789462570627
Publication statusPublished - 2014

Fingerprint

guard cells
vapor pressure
signal transduction
stomata
desiccation (plant physiology)
abscisic acid
Arabidopsis thaliana
calcium
leaves
chronic exposure
faba beans
Vicia faba
ligases

Keywords

  • arabidopsis thaliana
  • vicia faba
  • vapour pressure
  • desiccation
  • stomata
  • abscisic acid
  • signal transduction
  • plant physiology

Cite this

Ali Niaei Fard, S.. / Signal transduction pathway(s) in guard cells after prolonged exposure to low vapour pressure deficit. Wageningen : Wageningen University, 2014. 167 p.
@phdthesis{b4d79ce5771f49b2b21f9b9826bbb0b1,
title = "Signal transduction pathway(s) in guard cells after prolonged exposure to low vapour pressure deficit",
abstract = "Keywords: Abscisic acid, Arabidopsis thaliana, calcium, CYP707As, desiccation, environmental factors, guard cells’ signalling pathway, hydrogen peroxide, natural variation, nitric oxide, photosystem II efficiency, RD29A, relative water content, secondary messengers, stomata, vapour pressure deficit, Vicia faba In short-term, guard cells close stomata in response to an increase in vapour pressure deficit (VPD) and they open the stomata after exposure to low VPDs. However, in long-term responses to low VPD, adaptation processes occur which make stomata less sensitive to stimuli which usually induce stomatal closure (stomatal malfunctioning). Cellular mechanism(s) leading to occurrence of stomatal malfunctioning is (are) still unknown. The aim of this project was to elucidate the processes that are involved in the malfunctioning of stomata after long-term exposure to low VPD. To elucidate whether the problem of stomatal malfunctioning is due to alterations in stomatal morphology and leaf anatomy or in the ABA signalling pathway, fava bean plants were grown at low or moderate VPDs and some plants that developed their leaves at moderate VPD were then transferred for four days to low VPD. Leaf anatomical and stomatal morphological alterations due to low VPD were not the main reason of stomatal malfunctioning in response to ABA and desiccation. Within one day exposure to low VPD, the level of foliar ABA decreased to the same level as in low VPD-grown plants, while the level of ABA-glucose ester was not affected. Spraying ABA during a 4-day exposure to low VPD maintained closing ability of the stomata after 4-day low VPD-exposure. Therefore, alteration in the signalling pathways due to low foliar ABA level was recognized as the main reason for stomatal malfunctioning after long-term low VPD-exposure. Coincidence in changes of Ca2+, ABA receptors, and positive and negative regulators of ABA signalling are proposed as early steps for stomatal malfunctioning induced by low VPD-exposure. Transcriptional activators, transcriptional repressors as well as E3 ligases are proposed for long-term adaptation of cellular processes which consequently cause decreased stomatal response to closing stimuli afterwards. In order to find the molecular mechanism(s) of stomatal malfunctioning, possible variation in stomatal response to closing stimuli was studied among Arabidopsis thaliana accessions after a 4-day low VPD-exposure. Accessions could be grouped to very sensitive, moderately sensitive and less sensitive to closing stimuli using principle component analysis. A positive correlation was found between foliar ABA level (before desiccation) and stomatal closure response to ABA (but not to desiccation) after exposure to different VPDs. Stomatal response to desiccation was positively correlated with the foliar ABA level after desiccation. In order to elucidate the molecular network underlying stomatal malfunctioning in response to ABA due to long-term low VPD-exposure, two groups of Arabidopsis accessions were used as accessions that maintained responsiveness to ABA after low VPD-exposure and accessions with low VPD induced non-ABA-responsive stomata. The foliar ABA content in all accessions correlated with the stomatal response to ABA: only when the ABA level was above a threshold value, stomata responded to ABA. After low VPD-exposure, mainly due to catabolism of ABA, the foliar ABA content decreased. This decrease in ABA level resulted in down regulation of RD29A, which caused decreased stomatal responsiveness to ABA.  ",
keywords = "arabidopsis thaliana, vicia faba, dampdruk, verdroging, huidmondjes, abscisinezuur, signaaltransductie, plantenfysiologie, arabidopsis thaliana, vicia faba, vapour pressure, desiccation, stomata, abscisic acid, signal transduction, plant physiology",
author = "{Ali Niaei Fard}, S.",
note = "WU thesis 5821",
year = "2014",
language = "English",
isbn = "9789462570627",
publisher = "Wageningen University",
school = "Wageningen University",

}

Ali Niaei Fard, S 2014, 'Signal transduction pathway(s) in guard cells after prolonged exposure to low vapour pressure deficit', Doctor of Philosophy, Wageningen University, Wageningen.

Signal transduction pathway(s) in guard cells after prolonged exposure to low vapour pressure deficit. / Ali Niaei Fard, S.

Wageningen : Wageningen University, 2014. 167 p.

Research output: Thesisinternal PhD, WUAcademic

TY - THES

T1 - Signal transduction pathway(s) in guard cells after prolonged exposure to low vapour pressure deficit

AU - Ali Niaei Fard, S.

N1 - WU thesis 5821

PY - 2014

Y1 - 2014

N2 - Keywords: Abscisic acid, Arabidopsis thaliana, calcium, CYP707As, desiccation, environmental factors, guard cells’ signalling pathway, hydrogen peroxide, natural variation, nitric oxide, photosystem II efficiency, RD29A, relative water content, secondary messengers, stomata, vapour pressure deficit, Vicia faba In short-term, guard cells close stomata in response to an increase in vapour pressure deficit (VPD) and they open the stomata after exposure to low VPDs. However, in long-term responses to low VPD, adaptation processes occur which make stomata less sensitive to stimuli which usually induce stomatal closure (stomatal malfunctioning). Cellular mechanism(s) leading to occurrence of stomatal malfunctioning is (are) still unknown. The aim of this project was to elucidate the processes that are involved in the malfunctioning of stomata after long-term exposure to low VPD. To elucidate whether the problem of stomatal malfunctioning is due to alterations in stomatal morphology and leaf anatomy or in the ABA signalling pathway, fava bean plants were grown at low or moderate VPDs and some plants that developed their leaves at moderate VPD were then transferred for four days to low VPD. Leaf anatomical and stomatal morphological alterations due to low VPD were not the main reason of stomatal malfunctioning in response to ABA and desiccation. Within one day exposure to low VPD, the level of foliar ABA decreased to the same level as in low VPD-grown plants, while the level of ABA-glucose ester was not affected. Spraying ABA during a 4-day exposure to low VPD maintained closing ability of the stomata after 4-day low VPD-exposure. Therefore, alteration in the signalling pathways due to low foliar ABA level was recognized as the main reason for stomatal malfunctioning after long-term low VPD-exposure. Coincidence in changes of Ca2+, ABA receptors, and positive and negative regulators of ABA signalling are proposed as early steps for stomatal malfunctioning induced by low VPD-exposure. Transcriptional activators, transcriptional repressors as well as E3 ligases are proposed for long-term adaptation of cellular processes which consequently cause decreased stomatal response to closing stimuli afterwards. In order to find the molecular mechanism(s) of stomatal malfunctioning, possible variation in stomatal response to closing stimuli was studied among Arabidopsis thaliana accessions after a 4-day low VPD-exposure. Accessions could be grouped to very sensitive, moderately sensitive and less sensitive to closing stimuli using principle component analysis. A positive correlation was found between foliar ABA level (before desiccation) and stomatal closure response to ABA (but not to desiccation) after exposure to different VPDs. Stomatal response to desiccation was positively correlated with the foliar ABA level after desiccation. In order to elucidate the molecular network underlying stomatal malfunctioning in response to ABA due to long-term low VPD-exposure, two groups of Arabidopsis accessions were used as accessions that maintained responsiveness to ABA after low VPD-exposure and accessions with low VPD induced non-ABA-responsive stomata. The foliar ABA content in all accessions correlated with the stomatal response to ABA: only when the ABA level was above a threshold value, stomata responded to ABA. After low VPD-exposure, mainly due to catabolism of ABA, the foliar ABA content decreased. This decrease in ABA level resulted in down regulation of RD29A, which caused decreased stomatal responsiveness to ABA.  

AB - Keywords: Abscisic acid, Arabidopsis thaliana, calcium, CYP707As, desiccation, environmental factors, guard cells’ signalling pathway, hydrogen peroxide, natural variation, nitric oxide, photosystem II efficiency, RD29A, relative water content, secondary messengers, stomata, vapour pressure deficit, Vicia faba In short-term, guard cells close stomata in response to an increase in vapour pressure deficit (VPD) and they open the stomata after exposure to low VPDs. However, in long-term responses to low VPD, adaptation processes occur which make stomata less sensitive to stimuli which usually induce stomatal closure (stomatal malfunctioning). Cellular mechanism(s) leading to occurrence of stomatal malfunctioning is (are) still unknown. The aim of this project was to elucidate the processes that are involved in the malfunctioning of stomata after long-term exposure to low VPD. To elucidate whether the problem of stomatal malfunctioning is due to alterations in stomatal morphology and leaf anatomy or in the ABA signalling pathway, fava bean plants were grown at low or moderate VPDs and some plants that developed their leaves at moderate VPD were then transferred for four days to low VPD. Leaf anatomical and stomatal morphological alterations due to low VPD were not the main reason of stomatal malfunctioning in response to ABA and desiccation. Within one day exposure to low VPD, the level of foliar ABA decreased to the same level as in low VPD-grown plants, while the level of ABA-glucose ester was not affected. Spraying ABA during a 4-day exposure to low VPD maintained closing ability of the stomata after 4-day low VPD-exposure. Therefore, alteration in the signalling pathways due to low foliar ABA level was recognized as the main reason for stomatal malfunctioning after long-term low VPD-exposure. Coincidence in changes of Ca2+, ABA receptors, and positive and negative regulators of ABA signalling are proposed as early steps for stomatal malfunctioning induced by low VPD-exposure. Transcriptional activators, transcriptional repressors as well as E3 ligases are proposed for long-term adaptation of cellular processes which consequently cause decreased stomatal response to closing stimuli afterwards. In order to find the molecular mechanism(s) of stomatal malfunctioning, possible variation in stomatal response to closing stimuli was studied among Arabidopsis thaliana accessions after a 4-day low VPD-exposure. Accessions could be grouped to very sensitive, moderately sensitive and less sensitive to closing stimuli using principle component analysis. A positive correlation was found between foliar ABA level (before desiccation) and stomatal closure response to ABA (but not to desiccation) after exposure to different VPDs. Stomatal response to desiccation was positively correlated with the foliar ABA level after desiccation. In order to elucidate the molecular network underlying stomatal malfunctioning in response to ABA due to long-term low VPD-exposure, two groups of Arabidopsis accessions were used as accessions that maintained responsiveness to ABA after low VPD-exposure and accessions with low VPD induced non-ABA-responsive stomata. The foliar ABA content in all accessions correlated with the stomatal response to ABA: only when the ABA level was above a threshold value, stomata responded to ABA. After low VPD-exposure, mainly due to catabolism of ABA, the foliar ABA content decreased. This decrease in ABA level resulted in down regulation of RD29A, which caused decreased stomatal responsiveness to ABA.  

KW - arabidopsis thaliana

KW - vicia faba

KW - dampdruk

KW - verdroging

KW - huidmondjes

KW - abscisinezuur

KW - signaaltransductie

KW - plantenfysiologie

KW - arabidopsis thaliana

KW - vicia faba

KW - vapour pressure

KW - desiccation

KW - stomata

KW - abscisic acid

KW - signal transduction

KW - plant physiology

M3 - internal PhD, WU

SN - 9789462570627

PB - Wageningen University

CY - Wageningen

ER -