SIEVE ELEMENT-LINING CHAPERONE1 restricts aphid feeding on arabidopsis during heat stress

Karen J. Kloth*, Jacqueline Busscher-Lange, Gerrie L. Wiegers, Willem Kruijer, Gonda Buijs, Rhonda C. Meyer, Benedicte R. Albrectsen, Harro J. Bouwmeester, Marcel Dicke, Maarten A. Jongsma

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

12 Citations (Scopus)

Abstract

The role of phloem proteins in plant resistance to aphids is still largely elusive. By genome-wide association mapping of aphid behavior on 350 natural Arabidopsis thaliana accessions, we identified the small heat shock-like SIEVE ELEMENT-LINING CHAPERONE1 (SLI1). Detailed behavioral studies on near-isogenic and knockout lines showed that SLI1 impairs phloem feeding. Depending on the haplotype, aphids displayed a different duration of salivation in the phloem. On sli1 mutants, aphids prolonged their feeding sessions and ingested phloem at a higher rate than on wild-type plants. The largest phenotypic effects were observed at 26°C, when SLI1 expression is upregulated. At this moderately high temperature, sli1 mutants suffered from retarded elongation of the inflorescence and impaired silique development. Fluorescent reporter fusions showed that SLI1 is confined to the margins of sieve elements where it lines the parietal layer and colocalizes in spherical bodies around mitochondria. This localization pattern is reminiscent of the clamp-like structures observed in previous ultrastructural studies of the phloem and shows that the parietal phloem layer plays an important role in plant resistance to aphids and heat stress.
Original languageEnglish
Pages (from-to)2450-2464
JournalPlant Cell
Volume29
Issue number10
DOIs
Publication statusPublished - 1 Oct 2017

Fingerprint

Phloem
Aphids
sieve elements
Arabidopsis
phloem
heat stress
Aphidoidea
Hot Temperature
Salivation
Inflorescence
mutants
salivation
Plant Proteins
plant proteins
Haplotypes
chromosome mapping
Shock
haplotypes
Mitochondria
mitochondria

Cite this

Kloth, Karen J. ; Busscher-Lange, Jacqueline ; Wiegers, Gerrie L. ; Kruijer, Willem ; Buijs, Gonda ; Meyer, Rhonda C. ; Albrectsen, Benedicte R. ; Bouwmeester, Harro J. ; Dicke, Marcel ; Jongsma, Maarten A. / SIEVE ELEMENT-LINING CHAPERONE1 restricts aphid feeding on arabidopsis during heat stress. In: Plant Cell. 2017 ; Vol. 29, No. 10. pp. 2450-2464.
@article{91649b902aae48cb9080ef85cdc3b354,
title = "SIEVE ELEMENT-LINING CHAPERONE1 restricts aphid feeding on arabidopsis during heat stress",
abstract = "The role of phloem proteins in plant resistance to aphids is still largely elusive. By genome-wide association mapping of aphid behavior on 350 natural Arabidopsis thaliana accessions, we identified the small heat shock-like SIEVE ELEMENT-LINING CHAPERONE1 (SLI1). Detailed behavioral studies on near-isogenic and knockout lines showed that SLI1 impairs phloem feeding. Depending on the haplotype, aphids displayed a different duration of salivation in the phloem. On sli1 mutants, aphids prolonged their feeding sessions and ingested phloem at a higher rate than on wild-type plants. The largest phenotypic effects were observed at 26°C, when SLI1 expression is upregulated. At this moderately high temperature, sli1 mutants suffered from retarded elongation of the inflorescence and impaired silique development. Fluorescent reporter fusions showed that SLI1 is confined to the margins of sieve elements where it lines the parietal layer and colocalizes in spherical bodies around mitochondria. This localization pattern is reminiscent of the clamp-like structures observed in previous ultrastructural studies of the phloem and shows that the parietal phloem layer plays an important role in plant resistance to aphids and heat stress.",
author = "Kloth, {Karen J.} and Jacqueline Busscher-Lange and Wiegers, {Gerrie L.} and Willem Kruijer and Gonda Buijs and Meyer, {Rhonda C.} and Albrectsen, {Benedicte R.} and Bouwmeester, {Harro J.} and Marcel Dicke and Jongsma, {Maarten A.}",
year = "2017",
month = "10",
day = "1",
doi = "10.1105/tpc.16.00424",
language = "English",
volume = "29",
pages = "2450--2464",
journal = "The Plant Cell",
issn = "1040-4651",
publisher = "American Society of Plant Biologists",
number = "10",

}

SIEVE ELEMENT-LINING CHAPERONE1 restricts aphid feeding on arabidopsis during heat stress. / Kloth, Karen J.; Busscher-Lange, Jacqueline; Wiegers, Gerrie L.; Kruijer, Willem; Buijs, Gonda; Meyer, Rhonda C.; Albrectsen, Benedicte R.; Bouwmeester, Harro J.; Dicke, Marcel; Jongsma, Maarten A.

In: Plant Cell, Vol. 29, No. 10, 01.10.2017, p. 2450-2464.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - SIEVE ELEMENT-LINING CHAPERONE1 restricts aphid feeding on arabidopsis during heat stress

AU - Kloth, Karen J.

AU - Busscher-Lange, Jacqueline

AU - Wiegers, Gerrie L.

AU - Kruijer, Willem

AU - Buijs, Gonda

AU - Meyer, Rhonda C.

AU - Albrectsen, Benedicte R.

AU - Bouwmeester, Harro J.

AU - Dicke, Marcel

AU - Jongsma, Maarten A.

PY - 2017/10/1

Y1 - 2017/10/1

N2 - The role of phloem proteins in plant resistance to aphids is still largely elusive. By genome-wide association mapping of aphid behavior on 350 natural Arabidopsis thaliana accessions, we identified the small heat shock-like SIEVE ELEMENT-LINING CHAPERONE1 (SLI1). Detailed behavioral studies on near-isogenic and knockout lines showed that SLI1 impairs phloem feeding. Depending on the haplotype, aphids displayed a different duration of salivation in the phloem. On sli1 mutants, aphids prolonged their feeding sessions and ingested phloem at a higher rate than on wild-type plants. The largest phenotypic effects were observed at 26°C, when SLI1 expression is upregulated. At this moderately high temperature, sli1 mutants suffered from retarded elongation of the inflorescence and impaired silique development. Fluorescent reporter fusions showed that SLI1 is confined to the margins of sieve elements where it lines the parietal layer and colocalizes in spherical bodies around mitochondria. This localization pattern is reminiscent of the clamp-like structures observed in previous ultrastructural studies of the phloem and shows that the parietal phloem layer plays an important role in plant resistance to aphids and heat stress.

AB - The role of phloem proteins in plant resistance to aphids is still largely elusive. By genome-wide association mapping of aphid behavior on 350 natural Arabidopsis thaliana accessions, we identified the small heat shock-like SIEVE ELEMENT-LINING CHAPERONE1 (SLI1). Detailed behavioral studies on near-isogenic and knockout lines showed that SLI1 impairs phloem feeding. Depending on the haplotype, aphids displayed a different duration of salivation in the phloem. On sli1 mutants, aphids prolonged their feeding sessions and ingested phloem at a higher rate than on wild-type plants. The largest phenotypic effects were observed at 26°C, when SLI1 expression is upregulated. At this moderately high temperature, sli1 mutants suffered from retarded elongation of the inflorescence and impaired silique development. Fluorescent reporter fusions showed that SLI1 is confined to the margins of sieve elements where it lines the parietal layer and colocalizes in spherical bodies around mitochondria. This localization pattern is reminiscent of the clamp-like structures observed in previous ultrastructural studies of the phloem and shows that the parietal phloem layer plays an important role in plant resistance to aphids and heat stress.

U2 - 10.1105/tpc.16.00424

DO - 10.1105/tpc.16.00424

M3 - Article

VL - 29

SP - 2450

EP - 2464

JO - The Plant Cell

JF - The Plant Cell

SN - 1040-4651

IS - 10

ER -