Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 1013-1019 |
| Journal | Journal of Dairy Science |
| Volume | 101 |
| Issue number | 2 |
| Early online date | 15 Nov 2017 |
| DOIs | |
| Publication status | Published - Feb 2018 |
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Keywords
- Symbiosis
- Thermophile
- Thermoresistant spore
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Short communication : Growth of dairy isolates of Geobacillus thermoglucosidans in skim milk depends on lactose degradation products supplied by Anoxybacillus flavithermus as secondary species. / Zhao, Y.; Kumar, M.; Caspers, M.P.M.; Nierop Groot, M.N.; van der Vossen, J.M.B.M.; Abee, T.
In: Journal of Dairy Science, Vol. 101, No. 2, 02.2018, p. 1013-1019.Research output: Contribution to journal › Article › Academic › peer-review
TY - JOUR
T1 - Short communication
T2 - Growth of dairy isolates of Geobacillus thermoglucosidans in skim milk depends on lactose degradation products supplied by Anoxybacillus flavithermus as secondary species
AU - Zhao, Y.
AU - Kumar, M.
AU - Caspers, M.P.M.
AU - Nierop Groot, M.N.
AU - van der Vossen, J.M.B.M.
AU - Abee, T.
PY - 2018/2
Y1 - 2018/2
N2 - Thermophilic bacilli such as Anoxybacillus and Geobacillus are important contaminants in dairy powder products. Remarkably, one of the common contaminants, Geobacillus thermoglucosidans, showed poor growth in skim milk, whereas significant growth of G. thermoglucosidans was observed in the presence of an Anoxybacillus flavithermus dairy isolate. In the present study, we investigated the underlying reason for this growth dependence of G. thermoglucosidans. Whole-genome sequences of 4 A. flavithermus strains and 4 G. thermoglucosidans strains were acquired, with special attention given to carbohydrate utilization clusters and proteolytic enzymes. Focusing on traits relevant for dairy environments, comparative genomic analysis revealed that all G. thermoglucosidans strains lacked the genes necessary for lactose transport and metabolism, showed poor growth in skim milk, and produced white colonies on X-gal plates, indicating the lack of β-galactosidase activity. The A. flavithermus isolates scored positive in these tests, consistent with the presence of a putative lactose utilization gene cluster. All tested isolates from both species showed proteolytic activity on milk plate count agar plates. Adding glucose or galactose to liquid skim milk supported growth of G. thermoglucosidans isolates, in line with the presence of the respective monosaccharide utilization gene clusters in the genomes. Analysis by HPLC of A. flavithermus TNO-09.006 culture filtrate indicated that the previously described growth dependence of G. thermoglucosidans in skim milk was based on the supply of glucose and galactose by A. flavithermus TNO-09.006.
AB - Thermophilic bacilli such as Anoxybacillus and Geobacillus are important contaminants in dairy powder products. Remarkably, one of the common contaminants, Geobacillus thermoglucosidans, showed poor growth in skim milk, whereas significant growth of G. thermoglucosidans was observed in the presence of an Anoxybacillus flavithermus dairy isolate. In the present study, we investigated the underlying reason for this growth dependence of G. thermoglucosidans. Whole-genome sequences of 4 A. flavithermus strains and 4 G. thermoglucosidans strains were acquired, with special attention given to carbohydrate utilization clusters and proteolytic enzymes. Focusing on traits relevant for dairy environments, comparative genomic analysis revealed that all G. thermoglucosidans strains lacked the genes necessary for lactose transport and metabolism, showed poor growth in skim milk, and produced white colonies on X-gal plates, indicating the lack of β-galactosidase activity. The A. flavithermus isolates scored positive in these tests, consistent with the presence of a putative lactose utilization gene cluster. All tested isolates from both species showed proteolytic activity on milk plate count agar plates. Adding glucose or galactose to liquid skim milk supported growth of G. thermoglucosidans isolates, in line with the presence of the respective monosaccharide utilization gene clusters in the genomes. Analysis by HPLC of A. flavithermus TNO-09.006 culture filtrate indicated that the previously described growth dependence of G. thermoglucosidans in skim milk was based on the supply of glucose and galactose by A. flavithermus TNO-09.006.
KW - Symbiosis
KW - Thermophile
KW - Thermoresistant spore
U2 - 10.3168/jds.2017-13372
DO - 10.3168/jds.2017-13372
M3 - Article
VL - 101
SP - 1013
EP - 1019
JO - Journal of Dairy Science
JF - Journal of Dairy Science
SN - 0022-0302
IS - 2
ER -