Serum separation and structure of depletion- and bridging-flocculated emulsions: a comparison

T.B.J. Blijdenstein, A.J.M. van Winden, T. van Vliet, G.A. van Aken

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Stability against demixing, rheology and microstructure of emulsions that were flocculated by depletion or bridging were compared. Flocculation by depletion and bridging was induced by addition of the polysaccharide carboxy-methylcellulose (CMC) to emulsions that were stabilised by ß-lactoglobulin (ß-lg) at pH 6.7 and 3.0, respectively. Depletion-flocculated emulsions generally have a lower initial demixing rates than bridging-flocculated emulsions, but after long times they are compressed to a higher oil content by gravity. Differences in the initial demixing rate are shown to be caused by differences in porosity between the gels. In bridging-flocculated emulsions, large irreversible flocs are formed by flow during mixing, resulting in larger permeability than in depletion-flocculated emulsions. Rheological measurements showed that bridging-flocculated emulsions could withstand larger stresses than depletion-flocculated emulsions. Greater network strength and a lower probability of rearrangements explain why bridging-flocculation systems can retain more water at longer times. Keywords: Emulsions; Depletion; Bridging; Structure; Serum separation
Original languageEnglish
Pages (from-to)41-48
JournalColloids and Surfaces. A: Physicochemical and Engineering Aspects
Issue number1-3
Publication statusPublished - 2004


  • in-water emulsions
  • protein-stabilized emulsion
  • beta-lactoglobulin
  • polysaccharide
  • suspensions
  • carrageenan
  • interfaces
  • polymers
  • behavior
  • rheology


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