Serum-free mycoplasma growth medium

Erika Gaspari; Luis Garcia Morales; Vitor Alexandre Pires Martins Dos Santos

Serum-free mycoplasma growth medium

Erika Gaspari (Inventor)
, Luis Garcia Morales (Inventor)
, Vitor Alexandre Pires Martins Dos Santos (Inventor)

Research output: Patent

Abstract

The present invention provides a serum-free cell culture medium formulation that supports the in vitro cultivation of Mycoplasma species, especially of M. pneumoniae, and methods for cultivating Mycoplasma species in vitro, using these media. The media comprise a basal medium comprising at least one carbon source, vitamins, nucleotides, amino acids, and lipids including cholesterol, phospholipids and sphingolipids, and optionally palmitic acid and/or oleic acid. The invention further provides a use of phospholipids and sphingolipids to support growth of a Mycoplasma spp., preferably M. pneumoniae.

Original language	English
Patent number	WO2021086183
Priority date	28/10/19
Publication status	Published - 6 May 2021

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title = "Serum-free mycoplasma growth medium",

abstract = "The present invention provides a serum-free cell culture medium formulation that supports the in vitro cultivation of Mycoplasma species, especially of M. pneumoniae, and methods for cultivating Mycoplasma species in vitro, using these media. The media comprise a basal medium comprising at least one carbon source, vitamins, nucleotides, amino acids, and lipids including cholesterol, phospholipids and sphingolipids, and optionally palmitic acid and/or oleic acid. The invention further provides a use of phospholipids and sphingolipids to support growth of a Mycoplasma spp., preferably M. pneumoniae.",

author = "Erika Gaspari and \{Garcia Morales\}, Luis and \{Martins Dos Santos\}, \{Vitor Alexandre Pires\}",

year = "2021",

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TY - PAT

T1 - Serum-free mycoplasma growth medium

AU - Gaspari, Erika

AU - Garcia Morales, Luis

AU - Martins Dos Santos, Vitor Alexandre Pires

PY - 2021/5/6

Y1 - 2021/5/6

N2 - The present invention provides a serum-free cell culture medium formulation that supports the in vitro cultivation of Mycoplasma species, especially of M. pneumoniae, and methods for cultivating Mycoplasma species in vitro, using these media. The media comprise a basal medium comprising at least one carbon source, vitamins, nucleotides, amino acids, and lipids including cholesterol, phospholipids and sphingolipids, and optionally palmitic acid and/or oleic acid. The invention further provides a use of phospholipids and sphingolipids to support growth of a Mycoplasma spp., preferably M. pneumoniae.

AB - The present invention provides a serum-free cell culture medium formulation that supports the in vitro cultivation of Mycoplasma species, especially of M. pneumoniae, and methods for cultivating Mycoplasma species in vitro, using these media. The media comprise a basal medium comprising at least one carbon source, vitamins, nucleotides, amino acids, and lipids including cholesterol, phospholipids and sphingolipids, and optionally palmitic acid and/or oleic acid. The invention further provides a use of phospholipids and sphingolipids to support growth of a Mycoplasma spp., preferably M. pneumoniae.

M3 - Patent

M1 - WO2021086183

ER -

Serum-free mycoplasma growth medium

Abstract

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