Seroprevalence of antibodies against pestiviruses in small ruminants in the Netherlands

K. Orsel, A.F.G. Antonis, J.C. Oosterloo, P. Vellema, F.J.U.M. van der Meer

    Research output: Contribution to journalArticleAcademicpeer-review

    13 Citations (Scopus)


    In this study, a serological survey was performed to determine the prevalence of pestivirus (bovine viral diarrhea virus (BVDV) and border disease virus (BDV)) infected small ruminants herds in the Netherlands. After random selection of sheep farms, a sample size was determined to detect a 5% herd prevalence. 13 out of 29 farms were tested seropositive using an ELISA which detects antibodies directed against the non structural protein 3 (NS3) of pestiviruses. This resulted in a seroprevalence for the Netherlands of 45% [0.36; 0.54]. The within farm prevalence ranged from 4 till 65%. Using a virus neutralization assay, specific anti-BDV antibodies could be detected on two farms, while on one other farm anti-BVDV antibodies were present. On four farms antibodies to both viruses could be detected, on three of these farms antibodies against both viruses were equally present. At five farms that tested positive in the NS3-ELISA we were unable to detect pestivirus neutralizing antibodies in all sera using the VN test. This resulted in an estimated prevalence using the VN for the Netherlands of 28% [0.20; 0.60]. An additional survey in sera from dairy goats revealed that 34 out of 126 farms were serological positive resulting in a seroprevalence of 27% [0.23; 0.31], with a herd prevalence of 32% ranging from 1-100%.
    Original languageEnglish
    Pages (from-to)380-384
    JournalTijdschrift voor Diergeneeskunde
    Issue number9
    Publication statusPublished - 2009


    • antibodies
    • peste-des-petits-ruminants virus
    • seroprevalence
    • border disease virus
    • linked-immunosorbent-assay
    • bovine viral diarrhea
    • sheep
    • cattle
    • transmission
    • goats
    • herpesvirus-1
    • infection
    • bvdv


    Dive into the research topics of 'Seroprevalence of antibodies against pestiviruses in small ruminants in the Netherlands'. Together they form a unique fingerprint.

    Cite this