Serodiagnosis of Mycobacterium avium infections in pigs

H.J. Wisselink, C.B. Smits, D. Oorburg, D. Soolingen, P. Overduin, J.G. Maneschijn-Bonsing, N. Stockhofe, H. Buys-Bergen, B. Engel, B.A.P. Urlings, E.R. Jelle, J.E.R. Thole

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Abstract

The aim of this study is the development and evaluation of a serodiagnostic assay for Mycobacterium avium (MA). After screening MA lipid fractions in an ELISA format, a polar lipid fraction was selected as antigen because of its superior recognition by serum antibodies in experimentally infected pigs. The resulting MA-ELISA was evaluated as an alternative for detection of MA infection by traditional pathological examination of pig lymph nodes for granulomatous lesions by meat inspectors. By comparing with bacteriological examination, the MA-ELISA showed significantly better sensitivity (69%) as compared to pathological examination (31%) in experimentally infected pigs. The MA-ELISA also appeared significantly more specific in a set of serum samples from MA negative pigs: only 1 out of these 153 serum samples reacted positive, whereas 99 (65%) of these had displayed false positive results by detection of lymph nodes lesions that appeared not to be associated with MA (Komijn et al., 2007).The MA-ELISA was subsequently evaluated using serum samples from two farms with pigs known to be infected with MA. Bacteriological examination of the sub-maxillary and mesenteric lymph nodes showed that 56% (103/184) and 35% (41/117) of the pigs, respectively were positive for MA in these farms. In the first farm, 16% (29/184) of the pigs tested positive in MA-ELISA and 31% (57/184) by pathological examination. On the contrary, in the second farm, more pigs tested positive 17% (15/117) in MA-ELISA with 8% (9/117) positivity by pathological examination. Taking the results on both farms together, the sensitivity of the MA-ELISA was 14% and the specificity 83%, whereas the sensitivity of the pathological examination was 31% and the specificity 86%.For practical reasons use of a serological test as the MA-ELISA may be preferred over pathological or bacteriological examinations. Our studies in experimentally infected and negative “field” sera indicate that the MA-ELISA is significantly more specific and more sensitive than detection by classical pathological examination. However, the studies in two MA infected farms show a variable picture with pathological examination overall performing better. Study in a wider range of “positive” farms will be needed to provide a more comprehensive view of the quality of both tests for detection of MA in infected farms. At the same time further optimization of MA-ELISA with use of lipid antigens from a broader range of serotypes may improve its performance in the face of infections with different MA serotypes
Original languageEnglish
Pages (from-to)401-407
JournalVeterinary Microbiology
Volume142
Issue number3-4
DOIs
Publication statusPublished - 2010

Fingerprint

Mycobacterium avium
Mycobacterium Infections
serodiagnosis
Serologic Tests
Swine
swine
infection
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
farms
lymph nodes
Serum
Lymph Nodes
Lipids
lesions (animal)
serotypes
lipids
agricultural exhibitions
antigens
Antigens

Keywords

  • complex pulmonary-disease
  • slaughter pigs
  • lymph-nodes
  • glycopeptidolipid antigens
  • enzyme-immunoassay
  • rhodococcus-equi
  • subsp avium
  • prevalence
  • proposal
  • lesions

Cite this

Wisselink, H. J., Smits, C. B., Oorburg, D., Soolingen, D., Overduin, P., Maneschijn-Bonsing, J. G., ... Thole, J. E. R. (2010). Serodiagnosis of Mycobacterium avium infections in pigs. Veterinary Microbiology, 142(3-4), 401-407. https://doi.org/10.1016/j.vetmic.2009.11.003
Wisselink, H.J. ; Smits, C.B. ; Oorburg, D. ; Soolingen, D. ; Overduin, P. ; Maneschijn-Bonsing, J.G. ; Stockhofe, N. ; Buys-Bergen, H. ; Engel, B. ; Urlings, B.A.P. ; Jelle, E.R. ; Thole, J.E.R. / Serodiagnosis of Mycobacterium avium infections in pigs. In: Veterinary Microbiology. 2010 ; Vol. 142, No. 3-4. pp. 401-407.
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abstract = "The aim of this study is the development and evaluation of a serodiagnostic assay for Mycobacterium avium (MA). After screening MA lipid fractions in an ELISA format, a polar lipid fraction was selected as antigen because of its superior recognition by serum antibodies in experimentally infected pigs. The resulting MA-ELISA was evaluated as an alternative for detection of MA infection by traditional pathological examination of pig lymph nodes for granulomatous lesions by meat inspectors. By comparing with bacteriological examination, the MA-ELISA showed significantly better sensitivity (69{\%}) as compared to pathological examination (31{\%}) in experimentally infected pigs. The MA-ELISA also appeared significantly more specific in a set of serum samples from MA negative pigs: only 1 out of these 153 serum samples reacted positive, whereas 99 (65{\%}) of these had displayed false positive results by detection of lymph nodes lesions that appeared not to be associated with MA (Komijn et al., 2007).The MA-ELISA was subsequently evaluated using serum samples from two farms with pigs known to be infected with MA. Bacteriological examination of the sub-maxillary and mesenteric lymph nodes showed that 56{\%} (103/184) and 35{\%} (41/117) of the pigs, respectively were positive for MA in these farms. In the first farm, 16{\%} (29/184) of the pigs tested positive in MA-ELISA and 31{\%} (57/184) by pathological examination. On the contrary, in the second farm, more pigs tested positive 17{\%} (15/117) in MA-ELISA with 8{\%} (9/117) positivity by pathological examination. Taking the results on both farms together, the sensitivity of the MA-ELISA was 14{\%} and the specificity 83{\%}, whereas the sensitivity of the pathological examination was 31{\%} and the specificity 86{\%}.For practical reasons use of a serological test as the MA-ELISA may be preferred over pathological or bacteriological examinations. Our studies in experimentally infected and negative “field” sera indicate that the MA-ELISA is significantly more specific and more sensitive than detection by classical pathological examination. However, the studies in two MA infected farms show a variable picture with pathological examination overall performing better. Study in a wider range of “positive” farms will be needed to provide a more comprehensive view of the quality of both tests for detection of MA in infected farms. At the same time further optimization of MA-ELISA with use of lipid antigens from a broader range of serotypes may improve its performance in the face of infections with different MA serotypes",
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author = "H.J. Wisselink and C.B. Smits and D. Oorburg and D. Soolingen and P. Overduin and J.G. Maneschijn-Bonsing and N. Stockhofe and H. Buys-Bergen and B. Engel and B.A.P. Urlings and E.R. Jelle and J.E.R. Thole",
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Wisselink, HJ, Smits, CB, Oorburg, D, Soolingen, D, Overduin, P, Maneschijn-Bonsing, JG, Stockhofe, N, Buys-Bergen, H, Engel, B, Urlings, BAP, Jelle, ER & Thole, JER 2010, 'Serodiagnosis of Mycobacterium avium infections in pigs', Veterinary Microbiology, vol. 142, no. 3-4, pp. 401-407. https://doi.org/10.1016/j.vetmic.2009.11.003

Serodiagnosis of Mycobacterium avium infections in pigs. / Wisselink, H.J.; Smits, C.B.; Oorburg, D.; Soolingen, D.; Overduin, P.; Maneschijn-Bonsing, J.G.; Stockhofe, N.; Buys-Bergen, H.; Engel, B.; Urlings, B.A.P.; Jelle, E.R.; Thole, J.E.R.

In: Veterinary Microbiology, Vol. 142, No. 3-4, 2010, p. 401-407.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Serodiagnosis of Mycobacterium avium infections in pigs

AU - Wisselink, H.J.

AU - Smits, C.B.

AU - Oorburg, D.

AU - Soolingen, D.

AU - Overduin, P.

AU - Maneschijn-Bonsing, J.G.

AU - Stockhofe, N.

AU - Buys-Bergen, H.

AU - Engel, B.

AU - Urlings, B.A.P.

AU - Jelle, E.R.

AU - Thole, J.E.R.

PY - 2010

Y1 - 2010

N2 - The aim of this study is the development and evaluation of a serodiagnostic assay for Mycobacterium avium (MA). After screening MA lipid fractions in an ELISA format, a polar lipid fraction was selected as antigen because of its superior recognition by serum antibodies in experimentally infected pigs. The resulting MA-ELISA was evaluated as an alternative for detection of MA infection by traditional pathological examination of pig lymph nodes for granulomatous lesions by meat inspectors. By comparing with bacteriological examination, the MA-ELISA showed significantly better sensitivity (69%) as compared to pathological examination (31%) in experimentally infected pigs. The MA-ELISA also appeared significantly more specific in a set of serum samples from MA negative pigs: only 1 out of these 153 serum samples reacted positive, whereas 99 (65%) of these had displayed false positive results by detection of lymph nodes lesions that appeared not to be associated with MA (Komijn et al., 2007).The MA-ELISA was subsequently evaluated using serum samples from two farms with pigs known to be infected with MA. Bacteriological examination of the sub-maxillary and mesenteric lymph nodes showed that 56% (103/184) and 35% (41/117) of the pigs, respectively were positive for MA in these farms. In the first farm, 16% (29/184) of the pigs tested positive in MA-ELISA and 31% (57/184) by pathological examination. On the contrary, in the second farm, more pigs tested positive 17% (15/117) in MA-ELISA with 8% (9/117) positivity by pathological examination. Taking the results on both farms together, the sensitivity of the MA-ELISA was 14% and the specificity 83%, whereas the sensitivity of the pathological examination was 31% and the specificity 86%.For practical reasons use of a serological test as the MA-ELISA may be preferred over pathological or bacteriological examinations. Our studies in experimentally infected and negative “field” sera indicate that the MA-ELISA is significantly more specific and more sensitive than detection by classical pathological examination. However, the studies in two MA infected farms show a variable picture with pathological examination overall performing better. Study in a wider range of “positive” farms will be needed to provide a more comprehensive view of the quality of both tests for detection of MA in infected farms. At the same time further optimization of MA-ELISA with use of lipid antigens from a broader range of serotypes may improve its performance in the face of infections with different MA serotypes

AB - The aim of this study is the development and evaluation of a serodiagnostic assay for Mycobacterium avium (MA). After screening MA lipid fractions in an ELISA format, a polar lipid fraction was selected as antigen because of its superior recognition by serum antibodies in experimentally infected pigs. The resulting MA-ELISA was evaluated as an alternative for detection of MA infection by traditional pathological examination of pig lymph nodes for granulomatous lesions by meat inspectors. By comparing with bacteriological examination, the MA-ELISA showed significantly better sensitivity (69%) as compared to pathological examination (31%) in experimentally infected pigs. The MA-ELISA also appeared significantly more specific in a set of serum samples from MA negative pigs: only 1 out of these 153 serum samples reacted positive, whereas 99 (65%) of these had displayed false positive results by detection of lymph nodes lesions that appeared not to be associated with MA (Komijn et al., 2007).The MA-ELISA was subsequently evaluated using serum samples from two farms with pigs known to be infected with MA. Bacteriological examination of the sub-maxillary and mesenteric lymph nodes showed that 56% (103/184) and 35% (41/117) of the pigs, respectively were positive for MA in these farms. In the first farm, 16% (29/184) of the pigs tested positive in MA-ELISA and 31% (57/184) by pathological examination. On the contrary, in the second farm, more pigs tested positive 17% (15/117) in MA-ELISA with 8% (9/117) positivity by pathological examination. Taking the results on both farms together, the sensitivity of the MA-ELISA was 14% and the specificity 83%, whereas the sensitivity of the pathological examination was 31% and the specificity 86%.For practical reasons use of a serological test as the MA-ELISA may be preferred over pathological or bacteriological examinations. Our studies in experimentally infected and negative “field” sera indicate that the MA-ELISA is significantly more specific and more sensitive than detection by classical pathological examination. However, the studies in two MA infected farms show a variable picture with pathological examination overall performing better. Study in a wider range of “positive” farms will be needed to provide a more comprehensive view of the quality of both tests for detection of MA in infected farms. At the same time further optimization of MA-ELISA with use of lipid antigens from a broader range of serotypes may improve its performance in the face of infections with different MA serotypes

KW - complex pulmonary-disease

KW - slaughter pigs

KW - lymph-nodes

KW - glycopeptidolipid antigens

KW - enzyme-immunoassay

KW - rhodococcus-equi

KW - subsp avium

KW - prevalence

KW - proposal

KW - lesions

U2 - 10.1016/j.vetmic.2009.11.003

DO - 10.1016/j.vetmic.2009.11.003

M3 - Article

VL - 142

SP - 401

EP - 407

JO - Veterinary Microbiology

JF - Veterinary Microbiology

SN - 0378-1135

IS - 3-4

ER -