Sequence analysis and genomic organization of Aphid lethal paralysis virus: a new member of the family Dicistroviridae

M. van Munster, A.M. Dullemans, M. Verbeek, J.F.J.M. van den Heuvel, A. Clerivet, F. van der Wilk

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Abstract

The complete nucleotide sequence of the genomic RNA of an aphid-infecting virus, Aphid lethal paralysis virus (ALPV), has been determined. The genome is 9812 nt in length and contains two long open reading frames (ORFs), which are separated by an intergenic region of 163 nt. The first ORF (5' ORF) is preceded by an untranslated leader sequence of 506 nt, while an untranslated region of 571 nt follows the second ORF (3' ORF). The deduced amino acid sequences of the 5' ORF and 3' ORF products respectively showed similarity to the non-structural and structural proteins of members of the newly recognized genus Cripavirus (family Dicistroviridae). On the basis of the observed sequence similarities and identical genome organization, it is proposed that ALPV belongs to this genus. Phylogenetic analysis showed that ALPV is most closely related to Rhopalosiphum padi virus, and groups in a cluster with Drosophila C virus and Cricket paralysis virus, while the other members of this genus are more distantly related. Infectivity experiments showed that ALPV can not only infect aphid species but is also able to infect the whitefly Trialeurodes vaporariorum, extending its host range to another family of the order Hemiptera
Original languageEnglish
Pages (from-to)3131-3138
JournalJournal of General Virology
Volume83
Issue number12
Publication statusPublished - 2002

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Dicistroviridae
Aphids
Paralysis
Sequence Analysis
Open Reading Frames
Viruses
Hemiptera
Genome
Untranslated Regions
Gryllidae
Intergenic DNA
Host Specificity
Drosophila
Amino Acid Sequence

Cite this

van Munster, M. ; Dullemans, A.M. ; Verbeek, M. ; van den Heuvel, J.F.J.M. ; Clerivet, A. ; van der Wilk, F. / Sequence analysis and genomic organization of Aphid lethal paralysis virus: a new member of the family Dicistroviridae. In: Journal of General Virology. 2002 ; Vol. 83, No. 12. pp. 3131-3138.
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title = "Sequence analysis and genomic organization of Aphid lethal paralysis virus: a new member of the family Dicistroviridae",
abstract = "The complete nucleotide sequence of the genomic RNA of an aphid-infecting virus, Aphid lethal paralysis virus (ALPV), has been determined. The genome is 9812 nt in length and contains two long open reading frames (ORFs), which are separated by an intergenic region of 163 nt. The first ORF (5' ORF) is preceded by an untranslated leader sequence of 506 nt, while an untranslated region of 571 nt follows the second ORF (3' ORF). The deduced amino acid sequences of the 5' ORF and 3' ORF products respectively showed similarity to the non-structural and structural proteins of members of the newly recognized genus Cripavirus (family Dicistroviridae). On the basis of the observed sequence similarities and identical genome organization, it is proposed that ALPV belongs to this genus. Phylogenetic analysis showed that ALPV is most closely related to Rhopalosiphum padi virus, and groups in a cluster with Drosophila C virus and Cricket paralysis virus, while the other members of this genus are more distantly related. Infectivity experiments showed that ALPV can not only infect aphid species but is also able to infect the whitefly Trialeurodes vaporariorum, extending its host range to another family of the order Hemiptera",
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Sequence analysis and genomic organization of Aphid lethal paralysis virus: a new member of the family Dicistroviridae. / van Munster, M.; Dullemans, A.M.; Verbeek, M.; van den Heuvel, J.F.J.M.; Clerivet, A.; van der Wilk, F.

In: Journal of General Virology, Vol. 83, No. 12, 2002, p. 3131-3138.

Research output: Contribution to journalArticleAcademicpeer-review

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