Separation of Whey Proteins using Cascaded Ultrafiltration

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18 Citations (Scopus)


Whey protein isolate, containing a-Lactalbumin and ß-Lactoglobulin, was separated by using a continuous three-stage ultrafiltration cascade system. Single-stage experiments were optimized to enable good and stable cascade operation. Three different cascade configurations, a non-constrained ideal system (Configuration A), and adapted version (Configuration B), and a countercurrent cascade (Configuration C) were experimentally tested and compared. The countercurrent cascade system showed the traditional trade-off between yield and purity. Both the adapted cascade system and the non-constrained ideal cascade gave better performance in terms of recovery and purity and show potential for application, albeit for different purposes.
Original languageEnglish
Pages (from-to)2280-2288
JournalSeparation Science and Technology
Issue number15
Publication statusPublished - 2014


  • tangential flow filtration
  • membrane cascades
  • nanofiltration cascades
  • monoclonal-antibody
  • bed chromatography
  • beta-lactoglobulin
  • fractionation
  • purification
  • oligosaccharides
  • configuration


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