TY - JOUR
T1 - Separation of seventeen 2,3,7,8-substituted polychlorinated dibenzo-p-dioxins and dibenzofurans and 12 dioxin-like polychlorinated biphenyls by comprehensive two-dimensional gas chromatography with electron-capture detection
AU - Korytar, P.
AU - Danielsson, C.
AU - Leonards, P.E.G.
AU - Haglund, P.
AU - de Boer, J.
AU - Brinkman, U.A.Th.
PY - 2004
Y1 - 2004
N2 - Comprehensive two-dimensional gas chromatography (GCxGC) with electron-capture detection (ECD) has been optimized for the separation of seventeen 2,3,7,8-substituted polychlorinated dibenzo-p-dioxins and dibenzofurans and 12 dioxin-like polychlorinated biphenyls, with emphasis on the selection of the first- and second-dimension, commercially available, columns. When eight second-dimension columns were subsequently combined with a 100 ethylpolysiloxane stationary phase (DB-1) in the first dimension to create orthogonal conditions, a complete separation of all congeners with different TEF values was obtained with two column combinations, DB-1 x VF-23 and DB-1 x LC-50. When other types of first-dimension columns were used (and orthogonality was partly sacrificed), a DB-XLB column combined with 007-65HT, VF-23 and LC-50 was found to provide a complete separation of all 29 priority congeners. Next, the potential of these three column combinations for real-life analysis was preliminarily studied. With a spiked and fractionated milk extract, DB-XLB x LC-50 was found to be the most powerful column combination, because of the good separation of the 29 priority congeners from each other as well as from the matrix constituents. Quantitative performance (close to three-order linearity; LODs, 30 150 fg injected; R.S.D.s, 1.5 6.5¢n=10)) was satisfactory
AB - Comprehensive two-dimensional gas chromatography (GCxGC) with electron-capture detection (ECD) has been optimized for the separation of seventeen 2,3,7,8-substituted polychlorinated dibenzo-p-dioxins and dibenzofurans and 12 dioxin-like polychlorinated biphenyls, with emphasis on the selection of the first- and second-dimension, commercially available, columns. When eight second-dimension columns were subsequently combined with a 100 ethylpolysiloxane stationary phase (DB-1) in the first dimension to create orthogonal conditions, a complete separation of all congeners with different TEF values was obtained with two column combinations, DB-1 x VF-23 and DB-1 x LC-50. When other types of first-dimension columns were used (and orthogonality was partly sacrificed), a DB-XLB column combined with 007-65HT, VF-23 and LC-50 was found to provide a complete separation of all 29 priority congeners. Next, the potential of these three column combinations for real-life analysis was preliminarily studied. With a spiked and fractionated milk extract, DB-XLB x LC-50 was found to be the most powerful column combination, because of the good separation of the 29 priority congeners from each other as well as from the matrix constituents. Quantitative performance (close to three-order linearity; LODs, 30 150 fg injected; R.S.D.s, 1.5 6.5¢n=10)) was satisfactory
KW - contamination
KW - catalonia
KW - congeners
KW - samples
KW - spain
KW - pcbs
U2 - 10.1016/j.chroma.2004.03.026
DO - 10.1016/j.chroma.2004.03.026
M3 - Article
SN - 0021-9673
VL - 1038
SP - 189
EP - 199
JO - Journal of Chromatography. A, Including electrophoresis and other separation methods
JF - Journal of Chromatography. A, Including electrophoresis and other separation methods
ER -