Separation of isomeric cereal-derived arabinoxylan-oligosaccharides by collision induced dissociation-travelling wave ion mobility spectrometry-tandem mass spectrometry (CID-TWIMS-MS/MS)

Minna Juvonen*, Edwin Bakx, Henk Schols, Maija Tenkanen

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

Abstract

The potential of travelling wave ion mobility spectroscopy in combination with collision induced dissociation tandem mass spectrometry (CID-TWIMS-MS/MS) to separate cereal-derived isomeric arabinoxylan-oligosaccharides (A)XOS was investigated. Three trisaccharide, four tetrasaccharide, and four pentasaccharide (A)XOS isomers were analyzed by positive and negative ionization TWIMS-MS and CID-TWIMS-MS/MS. The tri- and pentasaccharide isomers were distinguishable by the ATDs of the precursor ions. The CID-TWIMS-MS/MS could separate most of the isomeric fragment ions produced from tetra- and pentasaccharide (A)XOS. Finally, the base peak mobility spectrum is introduced as a practical tool for (A)XOS fingerprinting.

Original languageEnglish
Article number130544
JournalFood Chemistry
Volume366
DOIs
Publication statusPublished - 1 Jan 2022

Keywords

  • Arabinoxylan
  • Ion mobility spectrometry
  • Oligosaccharides
  • Tandem mass spectrometry

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