Seminal plasma amino acid profile in different breeds of chicken

Role of seminal plasma on sperm cryoresistance

Julián Santiago-Moreno*, Berenice Bernal, Serafín Pérez-Cerezales, Cristina Castaño, Adolfo Toledano-Díaz, Milagros C. Esteso, Alfonso Gutiérrez-Adán, Antonio López-Sebastián, María G. Gil, Henri Woelders, Elisabeth Blesbois

*Corresponding author for this work

Research output: Contribution to journalArticleAcademicpeer-review

4 Citations (Scopus)

Abstract

Seminal plasma is a key biological fluid that modulates sperm function in the reproduction process. However, its role in sperm biotechnologies is scarce in poultry. The aims of the present study were to study the amino acids profile and total proteins of seminal plasma in 12 Spanish chicken breeds and to investigate the role of seminal plasma on cryoresistance of rooster sperm. To investigate the role of seminal plasma on cryoresistance, diluted pooled semen samples were cryopreserved in the presence and absence of seminal plasma. Glutamic acid was the most abundant free amino acid in seminal plasma, followed by alanine, serine, valine, and glycine. There was an influence of breed (P<0.05) on the percentage of viable sperm after freezing-thawing of samples with seminal plasma. Cluster analysis revealed that White Prat, Black Castellana, Blue Andaluza, Quail Castellana, and Red-Barred Vasca returned the best freezing-thawing response (good freezers). There was a positive correlation between seminal plasma concentrations of valine, isoleucine lysine, leucine and post thaw viability. The evaluation of fertilization capacity of frozen-thawed semen from the breeds White Prat (‘good freezer’) and Black-Red Andaluza (‘bad freezer’) showed that good freezer had higher fertility (20/68, 29.4%) compared to bad freezer breed (14/76, 18.4%), even if the difference was not significant (P = 0.08). The TUNEL assay revealed that freezing/thawing procedures in presence of seminal plasma provoked higher DNA fragmentation in most of the breeds, with a positive correlation between seminal alanine, valine, isoleucine, methionine, leucine, tyrosine, phenylalanine concentrations and DNA integrity. DNA fragmentation was lower in absence of seminal plasma and the breed effect on sperm viability was highly reduced. It is concluded that specific seminal plasma amino acids were associated with post-thaw percentage of viable sperm and DNA integrity. The removal of seminal plasma decreases the variability of the results and DNA fragmentation damages.

Original languageEnglish
Article numbere0209910
JournalPLoS ONE
Volume14
Issue number1
DOIs
Publication statusPublished - 1 Jan 2019

Fingerprint

chicken breeds
seminal plasma
Semen
Spermatozoa
Chickens
spermatozoa
Plasmas
Amino Acids
amino acids
freezers
Thawing
freeze-thaw cycles
breeds
DNA fragmentation
Valine
valine
DNA Fragmentation
Freezing
DNA
Isoleucine

Cite this

Santiago-Moreno, J., Bernal, B., Pérez-Cerezales, S., Castaño, C., Toledano-Díaz, A., Esteso, M. C., ... Blesbois, E. (2019). Seminal plasma amino acid profile in different breeds of chicken: Role of seminal plasma on sperm cryoresistance. PLoS ONE, 14(1), [e0209910]. https://doi.org/10.1371/journal.pone.0209910
Santiago-Moreno, Julián ; Bernal, Berenice ; Pérez-Cerezales, Serafín ; Castaño, Cristina ; Toledano-Díaz, Adolfo ; Esteso, Milagros C. ; Gutiérrez-Adán, Alfonso ; López-Sebastián, Antonio ; Gil, María G. ; Woelders, Henri ; Blesbois, Elisabeth. / Seminal plasma amino acid profile in different breeds of chicken : Role of seminal plasma on sperm cryoresistance. In: PLoS ONE. 2019 ; Vol. 14, No. 1.
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title = "Seminal plasma amino acid profile in different breeds of chicken: Role of seminal plasma on sperm cryoresistance",
abstract = "Seminal plasma is a key biological fluid that modulates sperm function in the reproduction process. However, its role in sperm biotechnologies is scarce in poultry. The aims of the present study were to study the amino acids profile and total proteins of seminal plasma in 12 Spanish chicken breeds and to investigate the role of seminal plasma on cryoresistance of rooster sperm. To investigate the role of seminal plasma on cryoresistance, diluted pooled semen samples were cryopreserved in the presence and absence of seminal plasma. Glutamic acid was the most abundant free amino acid in seminal plasma, followed by alanine, serine, valine, and glycine. There was an influence of breed (P<0.05) on the percentage of viable sperm after freezing-thawing of samples with seminal plasma. Cluster analysis revealed that White Prat, Black Castellana, Blue Andaluza, Quail Castellana, and Red-Barred Vasca returned the best freezing-thawing response (good freezers). There was a positive correlation between seminal plasma concentrations of valine, isoleucine lysine, leucine and post thaw viability. The evaluation of fertilization capacity of frozen-thawed semen from the breeds White Prat (‘good freezer’) and Black-Red Andaluza (‘bad freezer’) showed that good freezer had higher fertility (20/68, 29.4{\%}) compared to bad freezer breed (14/76, 18.4{\%}), even if the difference was not significant (P = 0.08). The TUNEL assay revealed that freezing/thawing procedures in presence of seminal plasma provoked higher DNA fragmentation in most of the breeds, with a positive correlation between seminal alanine, valine, isoleucine, methionine, leucine, tyrosine, phenylalanine concentrations and DNA integrity. DNA fragmentation was lower in absence of seminal plasma and the breed effect on sperm viability was highly reduced. It is concluded that specific seminal plasma amino acids were associated with post-thaw percentage of viable sperm and DNA integrity. The removal of seminal plasma decreases the variability of the results and DNA fragmentation damages.",
author = "Juli{\'a}n Santiago-Moreno and Berenice Bernal and Seraf{\'i}n P{\'e}rez-Cerezales and Cristina Casta{\~n}o and Adolfo Toledano-D{\'i}az and Esteso, {Milagros C.} and Alfonso Guti{\'e}rrez-Ad{\'a}n and Antonio L{\'o}pez-Sebasti{\'a}n and Gil, {Mar{\'i}a G.} and Henri Woelders and Elisabeth Blesbois",
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Santiago-Moreno, J, Bernal, B, Pérez-Cerezales, S, Castaño, C, Toledano-Díaz, A, Esteso, MC, Gutiérrez-Adán, A, López-Sebastián, A, Gil, MG, Woelders, H & Blesbois, E 2019, 'Seminal plasma amino acid profile in different breeds of chicken: Role of seminal plasma on sperm cryoresistance', PLoS ONE, vol. 14, no. 1, e0209910. https://doi.org/10.1371/journal.pone.0209910

Seminal plasma amino acid profile in different breeds of chicken : Role of seminal plasma on sperm cryoresistance. / Santiago-Moreno, Julián; Bernal, Berenice; Pérez-Cerezales, Serafín; Castaño, Cristina; Toledano-Díaz, Adolfo; Esteso, Milagros C.; Gutiérrez-Adán, Alfonso; López-Sebastián, Antonio; Gil, María G.; Woelders, Henri; Blesbois, Elisabeth.

In: PLoS ONE, Vol. 14, No. 1, e0209910, 01.01.2019.

Research output: Contribution to journalArticleAcademicpeer-review

TY - JOUR

T1 - Seminal plasma amino acid profile in different breeds of chicken

T2 - Role of seminal plasma on sperm cryoresistance

AU - Santiago-Moreno, Julián

AU - Bernal, Berenice

AU - Pérez-Cerezales, Serafín

AU - Castaño, Cristina

AU - Toledano-Díaz, Adolfo

AU - Esteso, Milagros C.

AU - Gutiérrez-Adán, Alfonso

AU - López-Sebastián, Antonio

AU - Gil, María G.

AU - Woelders, Henri

AU - Blesbois, Elisabeth

PY - 2019/1/1

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N2 - Seminal plasma is a key biological fluid that modulates sperm function in the reproduction process. However, its role in sperm biotechnologies is scarce in poultry. The aims of the present study were to study the amino acids profile and total proteins of seminal plasma in 12 Spanish chicken breeds and to investigate the role of seminal plasma on cryoresistance of rooster sperm. To investigate the role of seminal plasma on cryoresistance, diluted pooled semen samples were cryopreserved in the presence and absence of seminal plasma. Glutamic acid was the most abundant free amino acid in seminal plasma, followed by alanine, serine, valine, and glycine. There was an influence of breed (P<0.05) on the percentage of viable sperm after freezing-thawing of samples with seminal plasma. Cluster analysis revealed that White Prat, Black Castellana, Blue Andaluza, Quail Castellana, and Red-Barred Vasca returned the best freezing-thawing response (good freezers). There was a positive correlation between seminal plasma concentrations of valine, isoleucine lysine, leucine and post thaw viability. The evaluation of fertilization capacity of frozen-thawed semen from the breeds White Prat (‘good freezer’) and Black-Red Andaluza (‘bad freezer’) showed that good freezer had higher fertility (20/68, 29.4%) compared to bad freezer breed (14/76, 18.4%), even if the difference was not significant (P = 0.08). The TUNEL assay revealed that freezing/thawing procedures in presence of seminal plasma provoked higher DNA fragmentation in most of the breeds, with a positive correlation between seminal alanine, valine, isoleucine, methionine, leucine, tyrosine, phenylalanine concentrations and DNA integrity. DNA fragmentation was lower in absence of seminal plasma and the breed effect on sperm viability was highly reduced. It is concluded that specific seminal plasma amino acids were associated with post-thaw percentage of viable sperm and DNA integrity. The removal of seminal plasma decreases the variability of the results and DNA fragmentation damages.

AB - Seminal plasma is a key biological fluid that modulates sperm function in the reproduction process. However, its role in sperm biotechnologies is scarce in poultry. The aims of the present study were to study the amino acids profile and total proteins of seminal plasma in 12 Spanish chicken breeds and to investigate the role of seminal plasma on cryoresistance of rooster sperm. To investigate the role of seminal plasma on cryoresistance, diluted pooled semen samples were cryopreserved in the presence and absence of seminal plasma. Glutamic acid was the most abundant free amino acid in seminal plasma, followed by alanine, serine, valine, and glycine. There was an influence of breed (P<0.05) on the percentage of viable sperm after freezing-thawing of samples with seminal plasma. Cluster analysis revealed that White Prat, Black Castellana, Blue Andaluza, Quail Castellana, and Red-Barred Vasca returned the best freezing-thawing response (good freezers). There was a positive correlation between seminal plasma concentrations of valine, isoleucine lysine, leucine and post thaw viability. The evaluation of fertilization capacity of frozen-thawed semen from the breeds White Prat (‘good freezer’) and Black-Red Andaluza (‘bad freezer’) showed that good freezer had higher fertility (20/68, 29.4%) compared to bad freezer breed (14/76, 18.4%), even if the difference was not significant (P = 0.08). The TUNEL assay revealed that freezing/thawing procedures in presence of seminal plasma provoked higher DNA fragmentation in most of the breeds, with a positive correlation between seminal alanine, valine, isoleucine, methionine, leucine, tyrosine, phenylalanine concentrations and DNA integrity. DNA fragmentation was lower in absence of seminal plasma and the breed effect on sperm viability was highly reduced. It is concluded that specific seminal plasma amino acids were associated with post-thaw percentage of viable sperm and DNA integrity. The removal of seminal plasma decreases the variability of the results and DNA fragmentation damages.

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DO - 10.1371/journal.pone.0209910

M3 - Article

VL - 14

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

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Santiago-Moreno J, Bernal B, Pérez-Cerezales S, Castaño C, Toledano-Díaz A, Esteso MC et al. Seminal plasma amino acid profile in different breeds of chicken: Role of seminal plasma on sperm cryoresistance. PLoS ONE. 2019 Jan 1;14(1). e0209910. https://doi.org/10.1371/journal.pone.0209910